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Capsaicin Inhibits Inflammation and Gastric Damage during H pylori Infection by Targeting NF-kB–miRNA Axis

Helicobacter pylori (H. pylori) infection is considered as one of the strongest risk factors for gastric disorders. Infection triggers several host pathways to elicit inflammation, which further proceeds towards gastric complications. The NF-kB pathway plays a central role in the upregulation of the...

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Autores principales: Saha, Kalyani, Sarkar, Deotima, Khan, Uzma, Karmakar, Bipul Chandra, Paul, Sangita, Mukhopadhyay, Asish K., Dutta, Shanta, Bhattacharya, Sushmita
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9227394/
https://www.ncbi.nlm.nih.gov/pubmed/35745495
http://dx.doi.org/10.3390/pathogens11060641
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author Saha, Kalyani
Sarkar, Deotima
Khan, Uzma
Karmakar, Bipul Chandra
Paul, Sangita
Mukhopadhyay, Asish K.
Dutta, Shanta
Bhattacharya, Sushmita
author_facet Saha, Kalyani
Sarkar, Deotima
Khan, Uzma
Karmakar, Bipul Chandra
Paul, Sangita
Mukhopadhyay, Asish K.
Dutta, Shanta
Bhattacharya, Sushmita
author_sort Saha, Kalyani
collection PubMed
description Helicobacter pylori (H. pylori) infection is considered as one of the strongest risk factors for gastric disorders. Infection triggers several host pathways to elicit inflammation, which further proceeds towards gastric complications. The NF-kB pathway plays a central role in the upregulation of the pro-inflammatory cytokines during infection. It also regulates the transcriptional network of several inflammatory cytokine genes. Hence, targeting NF-kB could be an important strategy to reduce pathogenesis. Moreover, treatment of H. pylori needs attention as current therapeutics lack efficacy due to antibiotic resistance, highlighting the need for alternative therapeutic approaches. In this study, we investigated the effects of capsaicin, a known NF-kB inhibitor in reducing inflammation and gastric complications during H. pylori infection. We observed that capsaicin reduced NF-kB activation and upregulation of cytokine genes in an in vivo mice model. Moreover, it affected NF-kB–miRNA interplay to repress inflammation and gastric damages. Capsaicin reduced the expression level of mir21 and mir223 along with the pro-inflammatory cytokines. The repression of miRNA further affected downstream targets such as e-cadherin and Akt. Our data represent the first evidence that treatment with capsaicin inhibits inflammation and induces antimicrobial activity during H. pylori infection. This alternative approach might open a new avenue in treating H. pylori infection, thus reducing gastric problems.
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spelling pubmed-92273942022-06-25 Capsaicin Inhibits Inflammation and Gastric Damage during H pylori Infection by Targeting NF-kB–miRNA Axis Saha, Kalyani Sarkar, Deotima Khan, Uzma Karmakar, Bipul Chandra Paul, Sangita Mukhopadhyay, Asish K. Dutta, Shanta Bhattacharya, Sushmita Pathogens Article Helicobacter pylori (H. pylori) infection is considered as one of the strongest risk factors for gastric disorders. Infection triggers several host pathways to elicit inflammation, which further proceeds towards gastric complications. The NF-kB pathway plays a central role in the upregulation of the pro-inflammatory cytokines during infection. It also regulates the transcriptional network of several inflammatory cytokine genes. Hence, targeting NF-kB could be an important strategy to reduce pathogenesis. Moreover, treatment of H. pylori needs attention as current therapeutics lack efficacy due to antibiotic resistance, highlighting the need for alternative therapeutic approaches. In this study, we investigated the effects of capsaicin, a known NF-kB inhibitor in reducing inflammation and gastric complications during H. pylori infection. We observed that capsaicin reduced NF-kB activation and upregulation of cytokine genes in an in vivo mice model. Moreover, it affected NF-kB–miRNA interplay to repress inflammation and gastric damages. Capsaicin reduced the expression level of mir21 and mir223 along with the pro-inflammatory cytokines. The repression of miRNA further affected downstream targets such as e-cadherin and Akt. Our data represent the first evidence that treatment with capsaicin inhibits inflammation and induces antimicrobial activity during H. pylori infection. This alternative approach might open a new avenue in treating H. pylori infection, thus reducing gastric problems. MDPI 2022-06-01 /pmc/articles/PMC9227394/ /pubmed/35745495 http://dx.doi.org/10.3390/pathogens11060641 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Saha, Kalyani
Sarkar, Deotima
Khan, Uzma
Karmakar, Bipul Chandra
Paul, Sangita
Mukhopadhyay, Asish K.
Dutta, Shanta
Bhattacharya, Sushmita
Capsaicin Inhibits Inflammation and Gastric Damage during H pylori Infection by Targeting NF-kB–miRNA Axis
title Capsaicin Inhibits Inflammation and Gastric Damage during H pylori Infection by Targeting NF-kB–miRNA Axis
title_full Capsaicin Inhibits Inflammation and Gastric Damage during H pylori Infection by Targeting NF-kB–miRNA Axis
title_fullStr Capsaicin Inhibits Inflammation and Gastric Damage during H pylori Infection by Targeting NF-kB–miRNA Axis
title_full_unstemmed Capsaicin Inhibits Inflammation and Gastric Damage during H pylori Infection by Targeting NF-kB–miRNA Axis
title_short Capsaicin Inhibits Inflammation and Gastric Damage during H pylori Infection by Targeting NF-kB–miRNA Axis
title_sort capsaicin inhibits inflammation and gastric damage during h pylori infection by targeting nf-kb–mirna axis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9227394/
https://www.ncbi.nlm.nih.gov/pubmed/35745495
http://dx.doi.org/10.3390/pathogens11060641
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