Cargando…

Assembly and Comparison of Ca. Neoehrlichia mikurensis Genomes

Ca. Neoehrlichia mikurensis is widely prevalent in I. ricinus across Europe and has been associated with human disease. However, diagnostic modalities are limited, and much is still unknown about its biology. Here, we present the first complete Ca. Neoehrlichia mikurensis genomes directly derived fr...

Descripción completa

Detalles Bibliográficos
Autores principales: Azagi, Tal, Dirks, Ron P., Yebra-Pimentel, Elena S., Schaap, Peter J., Koehorst, Jasper J., Esser, Helen J., Sprong, Hein
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9227406/
https://www.ncbi.nlm.nih.gov/pubmed/35744652
http://dx.doi.org/10.3390/microorganisms10061134
_version_ 1784734168447451136
author Azagi, Tal
Dirks, Ron P.
Yebra-Pimentel, Elena S.
Schaap, Peter J.
Koehorst, Jasper J.
Esser, Helen J.
Sprong, Hein
author_facet Azagi, Tal
Dirks, Ron P.
Yebra-Pimentel, Elena S.
Schaap, Peter J.
Koehorst, Jasper J.
Esser, Helen J.
Sprong, Hein
author_sort Azagi, Tal
collection PubMed
description Ca. Neoehrlichia mikurensis is widely prevalent in I. ricinus across Europe and has been associated with human disease. However, diagnostic modalities are limited, and much is still unknown about its biology. Here, we present the first complete Ca. Neoehrlichia mikurensis genomes directly derived from wildlife reservoir host tissues, using both long- and short-read sequencing technologies. This pragmatic approach provides an alternative to obtaining sufficient material from clinical cases, a difficult task for emerging infectious diseases, and to expensive and challenging bacterial isolation and culture methods. Both genomes exhibit a larger chromosome than the currently available Ca. Neoehrlichia mikurensis genomes and expand the ability to find new targets for the development of supportive laboratory diagnostics in the future. Moreover, this method could be utilized for other tick-borne pathogens that are difficult to culture.
format Online
Article
Text
id pubmed-9227406
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-92274062022-06-25 Assembly and Comparison of Ca. Neoehrlichia mikurensis Genomes Azagi, Tal Dirks, Ron P. Yebra-Pimentel, Elena S. Schaap, Peter J. Koehorst, Jasper J. Esser, Helen J. Sprong, Hein Microorganisms Article Ca. Neoehrlichia mikurensis is widely prevalent in I. ricinus across Europe and has been associated with human disease. However, diagnostic modalities are limited, and much is still unknown about its biology. Here, we present the first complete Ca. Neoehrlichia mikurensis genomes directly derived from wildlife reservoir host tissues, using both long- and short-read sequencing technologies. This pragmatic approach provides an alternative to obtaining sufficient material from clinical cases, a difficult task for emerging infectious diseases, and to expensive and challenging bacterial isolation and culture methods. Both genomes exhibit a larger chromosome than the currently available Ca. Neoehrlichia mikurensis genomes and expand the ability to find new targets for the development of supportive laboratory diagnostics in the future. Moreover, this method could be utilized for other tick-borne pathogens that are difficult to culture. MDPI 2022-05-31 /pmc/articles/PMC9227406/ /pubmed/35744652 http://dx.doi.org/10.3390/microorganisms10061134 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Azagi, Tal
Dirks, Ron P.
Yebra-Pimentel, Elena S.
Schaap, Peter J.
Koehorst, Jasper J.
Esser, Helen J.
Sprong, Hein
Assembly and Comparison of Ca. Neoehrlichia mikurensis Genomes
title Assembly and Comparison of Ca. Neoehrlichia mikurensis Genomes
title_full Assembly and Comparison of Ca. Neoehrlichia mikurensis Genomes
title_fullStr Assembly and Comparison of Ca. Neoehrlichia mikurensis Genomes
title_full_unstemmed Assembly and Comparison of Ca. Neoehrlichia mikurensis Genomes
title_short Assembly and Comparison of Ca. Neoehrlichia mikurensis Genomes
title_sort assembly and comparison of ca. neoehrlichia mikurensis genomes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9227406/
https://www.ncbi.nlm.nih.gov/pubmed/35744652
http://dx.doi.org/10.3390/microorganisms10061134
work_keys_str_mv AT azagital assemblyandcomparisonofcaneoehrlichiamikurensisgenomes
AT dirksronp assemblyandcomparisonofcaneoehrlichiamikurensisgenomes
AT yebrapimentelelenas assemblyandcomparisonofcaneoehrlichiamikurensisgenomes
AT schaappeterj assemblyandcomparisonofcaneoehrlichiamikurensisgenomes
AT koehorstjasperj assemblyandcomparisonofcaneoehrlichiamikurensisgenomes
AT esserhelenj assemblyandcomparisonofcaneoehrlichiamikurensisgenomes
AT spronghein assemblyandcomparisonofcaneoehrlichiamikurensisgenomes