Cargando…

Green Synthesis of Silver Nanoparticles Using Aqueous Citrus limon Zest Extract: Characterization and Evaluation of Their Antioxidant and Antimicrobial Properties

The current work concentrated on the green synthesis of silver nanoparticles (AgNPs) through the use of aqueous Citrus limon zest extract, optimizing the different experimental factors required for the formation and stability of AgNPs. The preparation of nanoparticles was confirmed by the observatio...

Descripción completa

Detalles Bibliográficos
Autores principales: Khane, Yasmina, Benouis, Khedidja, Albukhaty, Salim, Sulaiman, Ghassan M., Abomughaid, Mosleh M., Al Ali, Amer, Aouf, Djaber, Fenniche, Fares, Khane, Sofiane, Chaibi, Wahiba, Henni, Abdallah, Bouras, Hadj Daoud, Dizge, Nadir
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9227472/
https://www.ncbi.nlm.nih.gov/pubmed/35745352
http://dx.doi.org/10.3390/nano12122013
_version_ 1784734186244931584
author Khane, Yasmina
Benouis, Khedidja
Albukhaty, Salim
Sulaiman, Ghassan M.
Abomughaid, Mosleh M.
Al Ali, Amer
Aouf, Djaber
Fenniche, Fares
Khane, Sofiane
Chaibi, Wahiba
Henni, Abdallah
Bouras, Hadj Daoud
Dizge, Nadir
author_facet Khane, Yasmina
Benouis, Khedidja
Albukhaty, Salim
Sulaiman, Ghassan M.
Abomughaid, Mosleh M.
Al Ali, Amer
Aouf, Djaber
Fenniche, Fares
Khane, Sofiane
Chaibi, Wahiba
Henni, Abdallah
Bouras, Hadj Daoud
Dizge, Nadir
author_sort Khane, Yasmina
collection PubMed
description The current work concentrated on the green synthesis of silver nanoparticles (AgNPs) through the use of aqueous Citrus limon zest extract, optimizing the different experimental factors required for the formation and stability of AgNPs. The preparation of nanoparticles was confirmed by the observation of the color change of the mixture of silver nitrate, after the addition of the plant extract, from yellow to a reddish-brown colloidal suspension and was established by detecting the surface plasmon resonance band at 535.5 nm, utilizing UV-Visible analysis. The optimum conditions were found to be 1 mM of silver nitrate concentration, a 1:9 ratio extract of the mixture, and a 4 h incubation period. Fourier transform infrared spectroscopy spectrum indicated that the phytochemicals compounds present in Citrus limon zest extract had a fundamental effect on the production of AgNPs as a bio-reducing agent. The morphology, size, and elemental composition of AgNPs were investigated by zeta potential (ZP), dynamic light scattering (DLS), SEM, EDX, X-ray diffraction (XRD), and transmission electron microscopy (TEM) analysis, which showed crystalline spherical silver nanoparticles. In addition, the antimicrobial and antioxidant properties of this bioactive silver nanoparticle were also investigated. The AgNPs showed excellent antibacterial activity against one Gram-negative pathogens bacteria, Escherichia coli, and one Gram-positive bacteria, Staphylococcus aureus, as well as antifungal activity against Candida albicans. The obtained results indicate that the antioxidant activity of this nanoparticle is significant. This bioactive silver nanoparticle can be used in biomedical and pharmacological fields.
format Online
Article
Text
id pubmed-9227472
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-92274722022-06-25 Green Synthesis of Silver Nanoparticles Using Aqueous Citrus limon Zest Extract: Characterization and Evaluation of Their Antioxidant and Antimicrobial Properties Khane, Yasmina Benouis, Khedidja Albukhaty, Salim Sulaiman, Ghassan M. Abomughaid, Mosleh M. Al Ali, Amer Aouf, Djaber Fenniche, Fares Khane, Sofiane Chaibi, Wahiba Henni, Abdallah Bouras, Hadj Daoud Dizge, Nadir Nanomaterials (Basel) Article The current work concentrated on the green synthesis of silver nanoparticles (AgNPs) through the use of aqueous Citrus limon zest extract, optimizing the different experimental factors required for the formation and stability of AgNPs. The preparation of nanoparticles was confirmed by the observation of the color change of the mixture of silver nitrate, after the addition of the plant extract, from yellow to a reddish-brown colloidal suspension and was established by detecting the surface plasmon resonance band at 535.5 nm, utilizing UV-Visible analysis. The optimum conditions were found to be 1 mM of silver nitrate concentration, a 1:9 ratio extract of the mixture, and a 4 h incubation period. Fourier transform infrared spectroscopy spectrum indicated that the phytochemicals compounds present in Citrus limon zest extract had a fundamental effect on the production of AgNPs as a bio-reducing agent. The morphology, size, and elemental composition of AgNPs were investigated by zeta potential (ZP), dynamic light scattering (DLS), SEM, EDX, X-ray diffraction (XRD), and transmission electron microscopy (TEM) analysis, which showed crystalline spherical silver nanoparticles. In addition, the antimicrobial and antioxidant properties of this bioactive silver nanoparticle were also investigated. The AgNPs showed excellent antibacterial activity against one Gram-negative pathogens bacteria, Escherichia coli, and one Gram-positive bacteria, Staphylococcus aureus, as well as antifungal activity against Candida albicans. The obtained results indicate that the antioxidant activity of this nanoparticle is significant. This bioactive silver nanoparticle can be used in biomedical and pharmacological fields. MDPI 2022-06-10 /pmc/articles/PMC9227472/ /pubmed/35745352 http://dx.doi.org/10.3390/nano12122013 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Khane, Yasmina
Benouis, Khedidja
Albukhaty, Salim
Sulaiman, Ghassan M.
Abomughaid, Mosleh M.
Al Ali, Amer
Aouf, Djaber
Fenniche, Fares
Khane, Sofiane
Chaibi, Wahiba
Henni, Abdallah
Bouras, Hadj Daoud
Dizge, Nadir
Green Synthesis of Silver Nanoparticles Using Aqueous Citrus limon Zest Extract: Characterization and Evaluation of Their Antioxidant and Antimicrobial Properties
title Green Synthesis of Silver Nanoparticles Using Aqueous Citrus limon Zest Extract: Characterization and Evaluation of Their Antioxidant and Antimicrobial Properties
title_full Green Synthesis of Silver Nanoparticles Using Aqueous Citrus limon Zest Extract: Characterization and Evaluation of Their Antioxidant and Antimicrobial Properties
title_fullStr Green Synthesis of Silver Nanoparticles Using Aqueous Citrus limon Zest Extract: Characterization and Evaluation of Their Antioxidant and Antimicrobial Properties
title_full_unstemmed Green Synthesis of Silver Nanoparticles Using Aqueous Citrus limon Zest Extract: Characterization and Evaluation of Their Antioxidant and Antimicrobial Properties
title_short Green Synthesis of Silver Nanoparticles Using Aqueous Citrus limon Zest Extract: Characterization and Evaluation of Their Antioxidant and Antimicrobial Properties
title_sort green synthesis of silver nanoparticles using aqueous citrus limon zest extract: characterization and evaluation of their antioxidant and antimicrobial properties
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9227472/
https://www.ncbi.nlm.nih.gov/pubmed/35745352
http://dx.doi.org/10.3390/nano12122013
work_keys_str_mv AT khaneyasmina greensynthesisofsilvernanoparticlesusingaqueouscitruslimonzestextractcharacterizationandevaluationoftheirantioxidantandantimicrobialproperties
AT benouiskhedidja greensynthesisofsilvernanoparticlesusingaqueouscitruslimonzestextractcharacterizationandevaluationoftheirantioxidantandantimicrobialproperties
AT albukhatysalim greensynthesisofsilvernanoparticlesusingaqueouscitruslimonzestextractcharacterizationandevaluationoftheirantioxidantandantimicrobialproperties
AT sulaimanghassanm greensynthesisofsilvernanoparticlesusingaqueouscitruslimonzestextractcharacterizationandevaluationoftheirantioxidantandantimicrobialproperties
AT abomughaidmoslehm greensynthesisofsilvernanoparticlesusingaqueouscitruslimonzestextractcharacterizationandevaluationoftheirantioxidantandantimicrobialproperties
AT alaliamer greensynthesisofsilvernanoparticlesusingaqueouscitruslimonzestextractcharacterizationandevaluationoftheirantioxidantandantimicrobialproperties
AT aoufdjaber greensynthesisofsilvernanoparticlesusingaqueouscitruslimonzestextractcharacterizationandevaluationoftheirantioxidantandantimicrobialproperties
AT fennichefares greensynthesisofsilvernanoparticlesusingaqueouscitruslimonzestextractcharacterizationandevaluationoftheirantioxidantandantimicrobialproperties
AT khanesofiane greensynthesisofsilvernanoparticlesusingaqueouscitruslimonzestextractcharacterizationandevaluationoftheirantioxidantandantimicrobialproperties
AT chaibiwahiba greensynthesisofsilvernanoparticlesusingaqueouscitruslimonzestextractcharacterizationandevaluationoftheirantioxidantandantimicrobialproperties
AT henniabdallah greensynthesisofsilvernanoparticlesusingaqueouscitruslimonzestextractcharacterizationandevaluationoftheirantioxidantandantimicrobialproperties
AT bourashadjdaoud greensynthesisofsilvernanoparticlesusingaqueouscitruslimonzestextractcharacterizationandevaluationoftheirantioxidantandantimicrobialproperties
AT dizgenadir greensynthesisofsilvernanoparticlesusingaqueouscitruslimonzestextractcharacterizationandevaluationoftheirantioxidantandantimicrobialproperties