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Characterization of Microbial Diversity in Decayed Wood from a Spanish Forest: An Environmental Source of Industrially Relevant Microorganisms

Rotting wood is inhabited by a large diversity of bacteria, fungi, and insects with complex environmental relationships. The aim of this work was to study the composition of the microbiota (bacteria and fungi) in decaying wood from a northwest Spanish forest as a source of industrially relevant micr...

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Autores principales: Velasco-Rodríguez, Óscar, Fil, Mariana, Heggeset, Tonje M. B., Degnes, Kristin F., Becerro-Recio, David, Kolsaková, Katarina, Haugen, Tone, Jønsson, Malene, Toral-Martínez, Macarena, García-Estrada, Carlos, Sola-Landa, Alberto, Josefsen, Kjell D., Sletta, Håvard, Barreiro, Carlos
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9227542/
https://www.ncbi.nlm.nih.gov/pubmed/35744767
http://dx.doi.org/10.3390/microorganisms10061249
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author Velasco-Rodríguez, Óscar
Fil, Mariana
Heggeset, Tonje M. B.
Degnes, Kristin F.
Becerro-Recio, David
Kolsaková, Katarina
Haugen, Tone
Jønsson, Malene
Toral-Martínez, Macarena
García-Estrada, Carlos
Sola-Landa, Alberto
Josefsen, Kjell D.
Sletta, Håvard
Barreiro, Carlos
author_facet Velasco-Rodríguez, Óscar
Fil, Mariana
Heggeset, Tonje M. B.
Degnes, Kristin F.
Becerro-Recio, David
Kolsaková, Katarina
Haugen, Tone
Jønsson, Malene
Toral-Martínez, Macarena
García-Estrada, Carlos
Sola-Landa, Alberto
Josefsen, Kjell D.
Sletta, Håvard
Barreiro, Carlos
author_sort Velasco-Rodríguez, Óscar
collection PubMed
description Rotting wood is inhabited by a large diversity of bacteria, fungi, and insects with complex environmental relationships. The aim of this work was to study the composition of the microbiota (bacteria and fungi) in decaying wood from a northwest Spanish forest as a source of industrially relevant microorganisms. The analyzed forest is situated in a well-defined biogeographic area combining Mediterranean and temperate macrobioclimates. Bacterial diversity, determined by metagenome analyses, was higher than fungal heterogeneity. However, a total of 194 different cultivable bacterial isolates (mainly Bacillaceae, Streptomycetaceae, Paenibacillaceae, and Microbacteriaceae) were obtained, in contrast to 343 fungal strains (mainly Aspergillaceae, Hypocreaceae, and Coniochaetaceae). Isolates traditionally known as secondary metabolite producers, such as Actinobacteria and members of the Penicillium genus, were screened for their antimicrobial activity by the detection of antibiotic biosynthetic clusters and competitive bioassays against fungi involved in wood decay. In addition, the ability of Penicillium isolates to degrade cellulose and release ferulic acid from wood was also examined. These results present decaying wood as an ecologically rich niche and a promising source of biotechnologically interesting microorganisms.
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spelling pubmed-92275422022-06-25 Characterization of Microbial Diversity in Decayed Wood from a Spanish Forest: An Environmental Source of Industrially Relevant Microorganisms Velasco-Rodríguez, Óscar Fil, Mariana Heggeset, Tonje M. B. Degnes, Kristin F. Becerro-Recio, David Kolsaková, Katarina Haugen, Tone Jønsson, Malene Toral-Martínez, Macarena García-Estrada, Carlos Sola-Landa, Alberto Josefsen, Kjell D. Sletta, Håvard Barreiro, Carlos Microorganisms Article Rotting wood is inhabited by a large diversity of bacteria, fungi, and insects with complex environmental relationships. The aim of this work was to study the composition of the microbiota (bacteria and fungi) in decaying wood from a northwest Spanish forest as a source of industrially relevant microorganisms. The analyzed forest is situated in a well-defined biogeographic area combining Mediterranean and temperate macrobioclimates. Bacterial diversity, determined by metagenome analyses, was higher than fungal heterogeneity. However, a total of 194 different cultivable bacterial isolates (mainly Bacillaceae, Streptomycetaceae, Paenibacillaceae, and Microbacteriaceae) were obtained, in contrast to 343 fungal strains (mainly Aspergillaceae, Hypocreaceae, and Coniochaetaceae). Isolates traditionally known as secondary metabolite producers, such as Actinobacteria and members of the Penicillium genus, were screened for their antimicrobial activity by the detection of antibiotic biosynthetic clusters and competitive bioassays against fungi involved in wood decay. In addition, the ability of Penicillium isolates to degrade cellulose and release ferulic acid from wood was also examined. These results present decaying wood as an ecologically rich niche and a promising source of biotechnologically interesting microorganisms. MDPI 2022-06-18 /pmc/articles/PMC9227542/ /pubmed/35744767 http://dx.doi.org/10.3390/microorganisms10061249 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Velasco-Rodríguez, Óscar
Fil, Mariana
Heggeset, Tonje M. B.
Degnes, Kristin F.
Becerro-Recio, David
Kolsaková, Katarina
Haugen, Tone
Jønsson, Malene
Toral-Martínez, Macarena
García-Estrada, Carlos
Sola-Landa, Alberto
Josefsen, Kjell D.
Sletta, Håvard
Barreiro, Carlos
Characterization of Microbial Diversity in Decayed Wood from a Spanish Forest: An Environmental Source of Industrially Relevant Microorganisms
title Characterization of Microbial Diversity in Decayed Wood from a Spanish Forest: An Environmental Source of Industrially Relevant Microorganisms
title_full Characterization of Microbial Diversity in Decayed Wood from a Spanish Forest: An Environmental Source of Industrially Relevant Microorganisms
title_fullStr Characterization of Microbial Diversity in Decayed Wood from a Spanish Forest: An Environmental Source of Industrially Relevant Microorganisms
title_full_unstemmed Characterization of Microbial Diversity in Decayed Wood from a Spanish Forest: An Environmental Source of Industrially Relevant Microorganisms
title_short Characterization of Microbial Diversity in Decayed Wood from a Spanish Forest: An Environmental Source of Industrially Relevant Microorganisms
title_sort characterization of microbial diversity in decayed wood from a spanish forest: an environmental source of industrially relevant microorganisms
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9227542/
https://www.ncbi.nlm.nih.gov/pubmed/35744767
http://dx.doi.org/10.3390/microorganisms10061249
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