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Analysis of the Expression of Neurotrophins and Their Receptors in Adult Zebrafish Kidney

Neurotrophins and their receptors are involved in the development and maintenance of neuronal populations. Different reports have shown that all neurotrophin/receptor pathways can also play a role in several non-neuronal tissues in vertebrates, including the kidney. These signaling pathways are invo...

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Autores principales: Cacialli, Pietro, Lucini, Carla
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9227799/
https://www.ncbi.nlm.nih.gov/pubmed/35737348
http://dx.doi.org/10.3390/vetsci9060296
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author Cacialli, Pietro
Lucini, Carla
author_facet Cacialli, Pietro
Lucini, Carla
author_sort Cacialli, Pietro
collection PubMed
description Neurotrophins and their receptors are involved in the development and maintenance of neuronal populations. Different reports have shown that all neurotrophin/receptor pathways can also play a role in several non-neuronal tissues in vertebrates, including the kidney. These signaling pathways are involved in different events to ensure the correct functioning of the kidney, such as growth, differentiation, and regulation of renal tubule transport. Previous studies in some fish species have identified the neurotrophins and receptors in the kidney. In this study, for the first time, we compare the expression profiles (mRNA and protein) of all neurotrophin/receptor pathways in the kidney of the adult zebrafish. We quantify the levels of mRNA by using qPCR and identify the expression pattern of each neurotrophin/receptor pathway by in situ hybridization. Next, we detect the proteins using Western blotting and immunohistochemistry. Our results show that among all neurotrophins analyzed, NT-3/TrkC is the most expressed in the glomerule and tubule and in the hematopoietic cells, similar to what has been reported in the mammalian kidney.
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spelling pubmed-92277992022-06-25 Analysis of the Expression of Neurotrophins and Their Receptors in Adult Zebrafish Kidney Cacialli, Pietro Lucini, Carla Vet Sci Article Neurotrophins and their receptors are involved in the development and maintenance of neuronal populations. Different reports have shown that all neurotrophin/receptor pathways can also play a role in several non-neuronal tissues in vertebrates, including the kidney. These signaling pathways are involved in different events to ensure the correct functioning of the kidney, such as growth, differentiation, and regulation of renal tubule transport. Previous studies in some fish species have identified the neurotrophins and receptors in the kidney. In this study, for the first time, we compare the expression profiles (mRNA and protein) of all neurotrophin/receptor pathways in the kidney of the adult zebrafish. We quantify the levels of mRNA by using qPCR and identify the expression pattern of each neurotrophin/receptor pathway by in situ hybridization. Next, we detect the proteins using Western blotting and immunohistochemistry. Our results show that among all neurotrophins analyzed, NT-3/TrkC is the most expressed in the glomerule and tubule and in the hematopoietic cells, similar to what has been reported in the mammalian kidney. MDPI 2022-06-15 /pmc/articles/PMC9227799/ /pubmed/35737348 http://dx.doi.org/10.3390/vetsci9060296 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Cacialli, Pietro
Lucini, Carla
Analysis of the Expression of Neurotrophins and Their Receptors in Adult Zebrafish Kidney
title Analysis of the Expression of Neurotrophins and Their Receptors in Adult Zebrafish Kidney
title_full Analysis of the Expression of Neurotrophins and Their Receptors in Adult Zebrafish Kidney
title_fullStr Analysis of the Expression of Neurotrophins and Their Receptors in Adult Zebrafish Kidney
title_full_unstemmed Analysis of the Expression of Neurotrophins and Their Receptors in Adult Zebrafish Kidney
title_short Analysis of the Expression of Neurotrophins and Their Receptors in Adult Zebrafish Kidney
title_sort analysis of the expression of neurotrophins and their receptors in adult zebrafish kidney
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9227799/
https://www.ncbi.nlm.nih.gov/pubmed/35737348
http://dx.doi.org/10.3390/vetsci9060296
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