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Analysis of the Expression of Neurotrophins and Their Receptors in Adult Zebrafish Kidney
Neurotrophins and their receptors are involved in the development and maintenance of neuronal populations. Different reports have shown that all neurotrophin/receptor pathways can also play a role in several non-neuronal tissues in vertebrates, including the kidney. These signaling pathways are invo...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9227799/ https://www.ncbi.nlm.nih.gov/pubmed/35737348 http://dx.doi.org/10.3390/vetsci9060296 |
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author | Cacialli, Pietro Lucini, Carla |
author_facet | Cacialli, Pietro Lucini, Carla |
author_sort | Cacialli, Pietro |
collection | PubMed |
description | Neurotrophins and their receptors are involved in the development and maintenance of neuronal populations. Different reports have shown that all neurotrophin/receptor pathways can also play a role in several non-neuronal tissues in vertebrates, including the kidney. These signaling pathways are involved in different events to ensure the correct functioning of the kidney, such as growth, differentiation, and regulation of renal tubule transport. Previous studies in some fish species have identified the neurotrophins and receptors in the kidney. In this study, for the first time, we compare the expression profiles (mRNA and protein) of all neurotrophin/receptor pathways in the kidney of the adult zebrafish. We quantify the levels of mRNA by using qPCR and identify the expression pattern of each neurotrophin/receptor pathway by in situ hybridization. Next, we detect the proteins using Western blotting and immunohistochemistry. Our results show that among all neurotrophins analyzed, NT-3/TrkC is the most expressed in the glomerule and tubule and in the hematopoietic cells, similar to what has been reported in the mammalian kidney. |
format | Online Article Text |
id | pubmed-9227799 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-92277992022-06-25 Analysis of the Expression of Neurotrophins and Their Receptors in Adult Zebrafish Kidney Cacialli, Pietro Lucini, Carla Vet Sci Article Neurotrophins and their receptors are involved in the development and maintenance of neuronal populations. Different reports have shown that all neurotrophin/receptor pathways can also play a role in several non-neuronal tissues in vertebrates, including the kidney. These signaling pathways are involved in different events to ensure the correct functioning of the kidney, such as growth, differentiation, and regulation of renal tubule transport. Previous studies in some fish species have identified the neurotrophins and receptors in the kidney. In this study, for the first time, we compare the expression profiles (mRNA and protein) of all neurotrophin/receptor pathways in the kidney of the adult zebrafish. We quantify the levels of mRNA by using qPCR and identify the expression pattern of each neurotrophin/receptor pathway by in situ hybridization. Next, we detect the proteins using Western blotting and immunohistochemistry. Our results show that among all neurotrophins analyzed, NT-3/TrkC is the most expressed in the glomerule and tubule and in the hematopoietic cells, similar to what has been reported in the mammalian kidney. MDPI 2022-06-15 /pmc/articles/PMC9227799/ /pubmed/35737348 http://dx.doi.org/10.3390/vetsci9060296 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Cacialli, Pietro Lucini, Carla Analysis of the Expression of Neurotrophins and Their Receptors in Adult Zebrafish Kidney |
title | Analysis of the Expression of Neurotrophins and Their Receptors in Adult Zebrafish Kidney |
title_full | Analysis of the Expression of Neurotrophins and Their Receptors in Adult Zebrafish Kidney |
title_fullStr | Analysis of the Expression of Neurotrophins and Their Receptors in Adult Zebrafish Kidney |
title_full_unstemmed | Analysis of the Expression of Neurotrophins and Their Receptors in Adult Zebrafish Kidney |
title_short | Analysis of the Expression of Neurotrophins and Their Receptors in Adult Zebrafish Kidney |
title_sort | analysis of the expression of neurotrophins and their receptors in adult zebrafish kidney |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9227799/ https://www.ncbi.nlm.nih.gov/pubmed/35737348 http://dx.doi.org/10.3390/vetsci9060296 |
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