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Impact of Skin Tissue Collection Method on Downstream MALDI-Imaging

MALDI imaging is a novel technique with which to study the pathophysiologies of diseases. Advancements in the field of metabolomics and lipidomics have been instrumental in mapping the signaling pathways involved in various diseases, such as cancer and neurodegenerative diseases (Parkinson’s). MALDI...

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Autores principales: Yadav, Manoj, Chaudhary, Prem Prashant, D’Souza, Brandon N., Spathies, Jacquelyn, Myles, Ian A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9227925/
https://www.ncbi.nlm.nih.gov/pubmed/35736430
http://dx.doi.org/10.3390/metabo12060497
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author Yadav, Manoj
Chaudhary, Prem Prashant
D’Souza, Brandon N.
Spathies, Jacquelyn
Myles, Ian A.
author_facet Yadav, Manoj
Chaudhary, Prem Prashant
D’Souza, Brandon N.
Spathies, Jacquelyn
Myles, Ian A.
author_sort Yadav, Manoj
collection PubMed
description MALDI imaging is a novel technique with which to study the pathophysiologies of diseases. Advancements in the field of metabolomics and lipidomics have been instrumental in mapping the signaling pathways involved in various diseases, such as cancer and neurodegenerative diseases (Parkinson’s). MALDI imaging is flexible and can handle many sample types. Researchers primarily use either formalin-fixed paraffin-embedded (FFPE) or fresh frozen tissue samples to answer their scientific questions. FFPE samples allow for easy long-term storage, but the requirement for extensive sample processing may limit the ability to provide a clear picture of metabolite distribution in biological tissue. Frozen samples require less handling, but present logistical challenges for collection and storage. A few studies, mostly focused on cancer cell lines, have directly compared the results of MALDI imaging using these two tissue fixation approaches. Herein, we directly compared FFPE and fresh frozen sample preparation for murine skin samples, and performed detailed pathway analysis to understand how differences in processing impact MALDI results from otherwise identical tissues. Our results indicate that FFPE and fresh frozen methods differ significantly in the putative identified metabolite content and distribution. The fixation methods shared only 2037 metabolites in positive mode and only 4079 metabolites in negative ion mode. However, both fixation approaches allowed for downstream fluorescent staining, which may save time and resources for samples that are clinically precious. This work represents a direct comparison of the impacts of the two main tissue processing methods on subsequent MALDI-MSI. While our results are similar to previous work in cancer tissue, they provide novel insights for those using MALDI-MSI in skin.
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spelling pubmed-92279252022-06-25 Impact of Skin Tissue Collection Method on Downstream MALDI-Imaging Yadav, Manoj Chaudhary, Prem Prashant D’Souza, Brandon N. Spathies, Jacquelyn Myles, Ian A. Metabolites Article MALDI imaging is a novel technique with which to study the pathophysiologies of diseases. Advancements in the field of metabolomics and lipidomics have been instrumental in mapping the signaling pathways involved in various diseases, such as cancer and neurodegenerative diseases (Parkinson’s). MALDI imaging is flexible and can handle many sample types. Researchers primarily use either formalin-fixed paraffin-embedded (FFPE) or fresh frozen tissue samples to answer their scientific questions. FFPE samples allow for easy long-term storage, but the requirement for extensive sample processing may limit the ability to provide a clear picture of metabolite distribution in biological tissue. Frozen samples require less handling, but present logistical challenges for collection and storage. A few studies, mostly focused on cancer cell lines, have directly compared the results of MALDI imaging using these two tissue fixation approaches. Herein, we directly compared FFPE and fresh frozen sample preparation for murine skin samples, and performed detailed pathway analysis to understand how differences in processing impact MALDI results from otherwise identical tissues. Our results indicate that FFPE and fresh frozen methods differ significantly in the putative identified metabolite content and distribution. The fixation methods shared only 2037 metabolites in positive mode and only 4079 metabolites in negative ion mode. However, both fixation approaches allowed for downstream fluorescent staining, which may save time and resources for samples that are clinically precious. This work represents a direct comparison of the impacts of the two main tissue processing methods on subsequent MALDI-MSI. While our results are similar to previous work in cancer tissue, they provide novel insights for those using MALDI-MSI in skin. MDPI 2022-05-30 /pmc/articles/PMC9227925/ /pubmed/35736430 http://dx.doi.org/10.3390/metabo12060497 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yadav, Manoj
Chaudhary, Prem Prashant
D’Souza, Brandon N.
Spathies, Jacquelyn
Myles, Ian A.
Impact of Skin Tissue Collection Method on Downstream MALDI-Imaging
title Impact of Skin Tissue Collection Method on Downstream MALDI-Imaging
title_full Impact of Skin Tissue Collection Method on Downstream MALDI-Imaging
title_fullStr Impact of Skin Tissue Collection Method on Downstream MALDI-Imaging
title_full_unstemmed Impact of Skin Tissue Collection Method on Downstream MALDI-Imaging
title_short Impact of Skin Tissue Collection Method on Downstream MALDI-Imaging
title_sort impact of skin tissue collection method on downstream maldi-imaging
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9227925/
https://www.ncbi.nlm.nih.gov/pubmed/35736430
http://dx.doi.org/10.3390/metabo12060497
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