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Gypenoside XVII, an Active Ingredient from Gynostemma Pentaphyllum, Inhibits C3aR-Associated Synaptic Pruning in Stressed Mice

Gynostemma pentaphyllum is a herbal medicine widely used in Asian countries, and its saponin extracts have been shown to possess potent anti-inflammatory effects. Gypenoside XVII, an active ingredient isolated from Gynostemma pentaphyllum, has been found to alleviate the inflammation induced by LPS...

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Autores principales: Zhang, Man-Man, Huo, Guo-Ming, Cheng, Jie, Zhang, Qiu-Ping, Li, Na-Zhi, Guo, Min-Xia, Liu, Qing, Xu, Guang-Hui, Zhu, Ji-Xiao, Li, Cheng-Fu, Zhou, Feng, Yi, Li-Tao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9228113/
https://www.ncbi.nlm.nih.gov/pubmed/35745148
http://dx.doi.org/10.3390/nu14122418
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author Zhang, Man-Man
Huo, Guo-Ming
Cheng, Jie
Zhang, Qiu-Ping
Li, Na-Zhi
Guo, Min-Xia
Liu, Qing
Xu, Guang-Hui
Zhu, Ji-Xiao
Li, Cheng-Fu
Zhou, Feng
Yi, Li-Tao
author_facet Zhang, Man-Man
Huo, Guo-Ming
Cheng, Jie
Zhang, Qiu-Ping
Li, Na-Zhi
Guo, Min-Xia
Liu, Qing
Xu, Guang-Hui
Zhu, Ji-Xiao
Li, Cheng-Fu
Zhou, Feng
Yi, Li-Tao
author_sort Zhang, Man-Man
collection PubMed
description Gynostemma pentaphyllum is a herbal medicine widely used in Asian countries, and its saponin extracts have been shown to possess potent anti-inflammatory effects. Gypenoside XVII, an active ingredient isolated from Gynostemma pentaphyllum, has been found to alleviate the inflammation induced by LPS in the BV2 microglia, according to our preliminary study. This study aims to evaluate whether Gypenoside XVII could attenuate depression-like symptoms in vivo and tries to demonstrate the involvement of the complement regulation in its antidepressant-like effect. The results showed that Gypenoside XVII significantly attenuated depression-like behaviors in the forced swimming test, tail suspension test and sucrose preference test. It also alleviated the acute stress-induced hyperactivity of serum corticosterone levels. Additionally, Gypenoside XVII significantly inhibited the activation of microglia and the expression of C3 in mice exposed to chronic unpredictable mild stress (CUMS). Meanwhile, the activation of C3aR/STAT3 signaling and the expression of proinflammatory cytokines was reversed by Gypenoside XVII. Moreover, CUMS induced excessive synaptic pruning by activating microglia, while Gypenoside XVII restored it in the prefrontal cortex. Our data demonstrated that Gypenoside XVII, the active ingredient of Gynostemma pentaphyllum, produced the antidepressant-like effects in mice, which was mediated by the inhibition of complement C3/C3aR/STAT3/cytokine signaling in the prefrontal cortex.
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spelling pubmed-92281132022-06-25 Gypenoside XVII, an Active Ingredient from Gynostemma Pentaphyllum, Inhibits C3aR-Associated Synaptic Pruning in Stressed Mice Zhang, Man-Man Huo, Guo-Ming Cheng, Jie Zhang, Qiu-Ping Li, Na-Zhi Guo, Min-Xia Liu, Qing Xu, Guang-Hui Zhu, Ji-Xiao Li, Cheng-Fu Zhou, Feng Yi, Li-Tao Nutrients Article Gynostemma pentaphyllum is a herbal medicine widely used in Asian countries, and its saponin extracts have been shown to possess potent anti-inflammatory effects. Gypenoside XVII, an active ingredient isolated from Gynostemma pentaphyllum, has been found to alleviate the inflammation induced by LPS in the BV2 microglia, according to our preliminary study. This study aims to evaluate whether Gypenoside XVII could attenuate depression-like symptoms in vivo and tries to demonstrate the involvement of the complement regulation in its antidepressant-like effect. The results showed that Gypenoside XVII significantly attenuated depression-like behaviors in the forced swimming test, tail suspension test and sucrose preference test. It also alleviated the acute stress-induced hyperactivity of serum corticosterone levels. Additionally, Gypenoside XVII significantly inhibited the activation of microglia and the expression of C3 in mice exposed to chronic unpredictable mild stress (CUMS). Meanwhile, the activation of C3aR/STAT3 signaling and the expression of proinflammatory cytokines was reversed by Gypenoside XVII. Moreover, CUMS induced excessive synaptic pruning by activating microglia, while Gypenoside XVII restored it in the prefrontal cortex. Our data demonstrated that Gypenoside XVII, the active ingredient of Gynostemma pentaphyllum, produced the antidepressant-like effects in mice, which was mediated by the inhibition of complement C3/C3aR/STAT3/cytokine signaling in the prefrontal cortex. MDPI 2022-06-10 /pmc/articles/PMC9228113/ /pubmed/35745148 http://dx.doi.org/10.3390/nu14122418 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhang, Man-Man
Huo, Guo-Ming
Cheng, Jie
Zhang, Qiu-Ping
Li, Na-Zhi
Guo, Min-Xia
Liu, Qing
Xu, Guang-Hui
Zhu, Ji-Xiao
Li, Cheng-Fu
Zhou, Feng
Yi, Li-Tao
Gypenoside XVII, an Active Ingredient from Gynostemma Pentaphyllum, Inhibits C3aR-Associated Synaptic Pruning in Stressed Mice
title Gypenoside XVII, an Active Ingredient from Gynostemma Pentaphyllum, Inhibits C3aR-Associated Synaptic Pruning in Stressed Mice
title_full Gypenoside XVII, an Active Ingredient from Gynostemma Pentaphyllum, Inhibits C3aR-Associated Synaptic Pruning in Stressed Mice
title_fullStr Gypenoside XVII, an Active Ingredient from Gynostemma Pentaphyllum, Inhibits C3aR-Associated Synaptic Pruning in Stressed Mice
title_full_unstemmed Gypenoside XVII, an Active Ingredient from Gynostemma Pentaphyllum, Inhibits C3aR-Associated Synaptic Pruning in Stressed Mice
title_short Gypenoside XVII, an Active Ingredient from Gynostemma Pentaphyllum, Inhibits C3aR-Associated Synaptic Pruning in Stressed Mice
title_sort gypenoside xvii, an active ingredient from gynostemma pentaphyllum, inhibits c3ar-associated synaptic pruning in stressed mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9228113/
https://www.ncbi.nlm.nih.gov/pubmed/35745148
http://dx.doi.org/10.3390/nu14122418
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