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Tumor-Homing of Mesenchymal Stem Cells Infected with Oncolytic Virus in a Canine Patient
Intravenous administration of oncolytic adenovirus (OAds) can be challenging, although various vehicles for the delivery of the virus to the tumor have been described. The efficacy of mesenchymal stem cells (MSCs) as a virus vehicle has been reported in mouse models and canine and human patients, bu...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9228126/ https://www.ncbi.nlm.nih.gov/pubmed/35737337 http://dx.doi.org/10.3390/vetsci9060285 |
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author | Delgado-Bonet, Pablo Tomeo-Martín, Beatriz Davinia Ortiz-Díez, Gustavo Perisé-Barrios, Ana Judith |
author_facet | Delgado-Bonet, Pablo Tomeo-Martín, Beatriz Davinia Ortiz-Díez, Gustavo Perisé-Barrios, Ana Judith |
author_sort | Delgado-Bonet, Pablo |
collection | PubMed |
description | Intravenous administration of oncolytic adenovirus (OAds) can be challenging, although various vehicles for the delivery of the virus to the tumor have been described. The efficacy of mesenchymal stem cells (MSCs) as a virus vehicle has been reported in mouse models and canine and human patients, but the actual action mechanism has never been described in patients. It is of importance to determine whether MSCs infected with OAds can reach the tumor and release the virus in a clinical setting. For this purpose, GFP-labeled MSCs were infected with an OAd and inoculated into a companion dog diagnosed with spontaneous lung carcinoma. Forty-eight hours later, the tumor was excised and analyzed microscopically by flow cytometry for GFP fluorescence detection, and a cellular culture was established. Peripheral blood samples were taken to quantify the oncolytic adenovirus by qRT-PCR. Green fluorescence cells detected in the cellular culture by microscopy and flow cytometry revealed 0.69% GFP-positive cells in the tumor. OAd in peripheral blood was confirmed by qRT-PCR during follow-up. For the first time, the tumoral-homing capacity of OAds infected-MSC has been confirmed in a clinical setting, helping to explain the clinical response mechanism, whose efficacy was previously reported in canine and human patients. |
format | Online Article Text |
id | pubmed-9228126 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-92281262022-06-25 Tumor-Homing of Mesenchymal Stem Cells Infected with Oncolytic Virus in a Canine Patient Delgado-Bonet, Pablo Tomeo-Martín, Beatriz Davinia Ortiz-Díez, Gustavo Perisé-Barrios, Ana Judith Vet Sci Case Report Intravenous administration of oncolytic adenovirus (OAds) can be challenging, although various vehicles for the delivery of the virus to the tumor have been described. The efficacy of mesenchymal stem cells (MSCs) as a virus vehicle has been reported in mouse models and canine and human patients, but the actual action mechanism has never been described in patients. It is of importance to determine whether MSCs infected with OAds can reach the tumor and release the virus in a clinical setting. For this purpose, GFP-labeled MSCs were infected with an OAd and inoculated into a companion dog diagnosed with spontaneous lung carcinoma. Forty-eight hours later, the tumor was excised and analyzed microscopically by flow cytometry for GFP fluorescence detection, and a cellular culture was established. Peripheral blood samples were taken to quantify the oncolytic adenovirus by qRT-PCR. Green fluorescence cells detected in the cellular culture by microscopy and flow cytometry revealed 0.69% GFP-positive cells in the tumor. OAd in peripheral blood was confirmed by qRT-PCR during follow-up. For the first time, the tumoral-homing capacity of OAds infected-MSC has been confirmed in a clinical setting, helping to explain the clinical response mechanism, whose efficacy was previously reported in canine and human patients. MDPI 2022-06-09 /pmc/articles/PMC9228126/ /pubmed/35737337 http://dx.doi.org/10.3390/vetsci9060285 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Case Report Delgado-Bonet, Pablo Tomeo-Martín, Beatriz Davinia Ortiz-Díez, Gustavo Perisé-Barrios, Ana Judith Tumor-Homing of Mesenchymal Stem Cells Infected with Oncolytic Virus in a Canine Patient |
title | Tumor-Homing of Mesenchymal Stem Cells Infected with Oncolytic Virus in a Canine Patient |
title_full | Tumor-Homing of Mesenchymal Stem Cells Infected with Oncolytic Virus in a Canine Patient |
title_fullStr | Tumor-Homing of Mesenchymal Stem Cells Infected with Oncolytic Virus in a Canine Patient |
title_full_unstemmed | Tumor-Homing of Mesenchymal Stem Cells Infected with Oncolytic Virus in a Canine Patient |
title_short | Tumor-Homing of Mesenchymal Stem Cells Infected with Oncolytic Virus in a Canine Patient |
title_sort | tumor-homing of mesenchymal stem cells infected with oncolytic virus in a canine patient |
topic | Case Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9228126/ https://www.ncbi.nlm.nih.gov/pubmed/35737337 http://dx.doi.org/10.3390/vetsci9060285 |
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