Cargando…

Tumor-Homing of Mesenchymal Stem Cells Infected with Oncolytic Virus in a Canine Patient

Intravenous administration of oncolytic adenovirus (OAds) can be challenging, although various vehicles for the delivery of the virus to the tumor have been described. The efficacy of mesenchymal stem cells (MSCs) as a virus vehicle has been reported in mouse models and canine and human patients, bu...

Descripción completa

Detalles Bibliográficos
Autores principales: Delgado-Bonet, Pablo, Tomeo-Martín, Beatriz Davinia, Ortiz-Díez, Gustavo, Perisé-Barrios, Ana Judith
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9228126/
https://www.ncbi.nlm.nih.gov/pubmed/35737337
http://dx.doi.org/10.3390/vetsci9060285
_version_ 1784734358425305088
author Delgado-Bonet, Pablo
Tomeo-Martín, Beatriz Davinia
Ortiz-Díez, Gustavo
Perisé-Barrios, Ana Judith
author_facet Delgado-Bonet, Pablo
Tomeo-Martín, Beatriz Davinia
Ortiz-Díez, Gustavo
Perisé-Barrios, Ana Judith
author_sort Delgado-Bonet, Pablo
collection PubMed
description Intravenous administration of oncolytic adenovirus (OAds) can be challenging, although various vehicles for the delivery of the virus to the tumor have been described. The efficacy of mesenchymal stem cells (MSCs) as a virus vehicle has been reported in mouse models and canine and human patients, but the actual action mechanism has never been described in patients. It is of importance to determine whether MSCs infected with OAds can reach the tumor and release the virus in a clinical setting. For this purpose, GFP-labeled MSCs were infected with an OAd and inoculated into a companion dog diagnosed with spontaneous lung carcinoma. Forty-eight hours later, the tumor was excised and analyzed microscopically by flow cytometry for GFP fluorescence detection, and a cellular culture was established. Peripheral blood samples were taken to quantify the oncolytic adenovirus by qRT-PCR. Green fluorescence cells detected in the cellular culture by microscopy and flow cytometry revealed 0.69% GFP-positive cells in the tumor. OAd in peripheral blood was confirmed by qRT-PCR during follow-up. For the first time, the tumoral-homing capacity of OAds infected-MSC has been confirmed in a clinical setting, helping to explain the clinical response mechanism, whose efficacy was previously reported in canine and human patients.
format Online
Article
Text
id pubmed-9228126
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-92281262022-06-25 Tumor-Homing of Mesenchymal Stem Cells Infected with Oncolytic Virus in a Canine Patient Delgado-Bonet, Pablo Tomeo-Martín, Beatriz Davinia Ortiz-Díez, Gustavo Perisé-Barrios, Ana Judith Vet Sci Case Report Intravenous administration of oncolytic adenovirus (OAds) can be challenging, although various vehicles for the delivery of the virus to the tumor have been described. The efficacy of mesenchymal stem cells (MSCs) as a virus vehicle has been reported in mouse models and canine and human patients, but the actual action mechanism has never been described in patients. It is of importance to determine whether MSCs infected with OAds can reach the tumor and release the virus in a clinical setting. For this purpose, GFP-labeled MSCs were infected with an OAd and inoculated into a companion dog diagnosed with spontaneous lung carcinoma. Forty-eight hours later, the tumor was excised and analyzed microscopically by flow cytometry for GFP fluorescence detection, and a cellular culture was established. Peripheral blood samples were taken to quantify the oncolytic adenovirus by qRT-PCR. Green fluorescence cells detected in the cellular culture by microscopy and flow cytometry revealed 0.69% GFP-positive cells in the tumor. OAd in peripheral blood was confirmed by qRT-PCR during follow-up. For the first time, the tumoral-homing capacity of OAds infected-MSC has been confirmed in a clinical setting, helping to explain the clinical response mechanism, whose efficacy was previously reported in canine and human patients. MDPI 2022-06-09 /pmc/articles/PMC9228126/ /pubmed/35737337 http://dx.doi.org/10.3390/vetsci9060285 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Case Report
Delgado-Bonet, Pablo
Tomeo-Martín, Beatriz Davinia
Ortiz-Díez, Gustavo
Perisé-Barrios, Ana Judith
Tumor-Homing of Mesenchymal Stem Cells Infected with Oncolytic Virus in a Canine Patient
title Tumor-Homing of Mesenchymal Stem Cells Infected with Oncolytic Virus in a Canine Patient
title_full Tumor-Homing of Mesenchymal Stem Cells Infected with Oncolytic Virus in a Canine Patient
title_fullStr Tumor-Homing of Mesenchymal Stem Cells Infected with Oncolytic Virus in a Canine Patient
title_full_unstemmed Tumor-Homing of Mesenchymal Stem Cells Infected with Oncolytic Virus in a Canine Patient
title_short Tumor-Homing of Mesenchymal Stem Cells Infected with Oncolytic Virus in a Canine Patient
title_sort tumor-homing of mesenchymal stem cells infected with oncolytic virus in a canine patient
topic Case Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9228126/
https://www.ncbi.nlm.nih.gov/pubmed/35737337
http://dx.doi.org/10.3390/vetsci9060285
work_keys_str_mv AT delgadobonetpablo tumorhomingofmesenchymalstemcellsinfectedwithoncolyticvirusinacaninepatient
AT tomeomartinbeatrizdavinia tumorhomingofmesenchymalstemcellsinfectedwithoncolyticvirusinacaninepatient
AT ortizdiezgustavo tumorhomingofmesenchymalstemcellsinfectedwithoncolyticvirusinacaninepatient
AT perisebarriosanajudith tumorhomingofmesenchymalstemcellsinfectedwithoncolyticvirusinacaninepatient