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The Optimization of Dietary Protein Level and Carbon Sources on Biofloc Nutritive Values, Bacterial Abundance, and Growth Performances of Whiteleg Shrimp (Litopenaeus vannamei) Juveniles

A biofloc technology-based 75-day indoor growth trial in an 80 L glass aquaria was conducted to evaluate the effects of two different carbon sources (sugarcane bagasse, SB, and wheat flour, WF) on the biofloc composition, bacterial abundance, and growth of whiteleg shrimp (Litopenaeus vannamei) juve...

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Autores principales: Mansour, Abdallah Tageldein, Ashry, Ola A., Ashour, Mohamed, Alsaqufi, Ahmed Saud, Ramadan, Khaled M. A., Sharawy, Zaki Z.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9228149/
https://www.ncbi.nlm.nih.gov/pubmed/35743919
http://dx.doi.org/10.3390/life12060888
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author Mansour, Abdallah Tageldein
Ashry, Ola A.
Ashour, Mohamed
Alsaqufi, Ahmed Saud
Ramadan, Khaled M. A.
Sharawy, Zaki Z.
author_facet Mansour, Abdallah Tageldein
Ashry, Ola A.
Ashour, Mohamed
Alsaqufi, Ahmed Saud
Ramadan, Khaled M. A.
Sharawy, Zaki Z.
author_sort Mansour, Abdallah Tageldein
collection PubMed
description A biofloc technology-based 75-day indoor growth trial in an 80 L glass aquaria was conducted to evaluate the effects of two different carbon sources (sugarcane bagasse, SB, and wheat flour, WF) on the biofloc composition, bacterial abundance, and growth of whiteleg shrimp (Litopenaeus vannamei) juveniles (0.23 ± 0.04 g). Three different levels of dietary protein content (250, 300, and 350 g protein kg(−1) diet) and two carbon sources (SB and WF) were applied (SB(250), WF(250), SB(300), WF(300), SB(350), and WF(350), respectively), comparing to a controlled diet without biofloc and fed on a 450 g protein kg(−1) diet (C(450)). With the addition of SB and WF, water quality was in the ideal recommended ranges for L. vannamei culture. At the end of the experiment, the biofloc volume increased with increasing dietary protein levels. The nutritional value of biofloc in different treatments was influenced by dietary protein and added SB and WF. Increasing dietary protein significantly increased the protein and lipid contents of the produced biofloc. The use of WF as a carbon source significantly increased lipids and nitrogen-free extract in the biofloc. The total heterotrophic bacterial (THB) count was significantly higher (p < 0.05) in WF(300) and WF(350) than in the other treatments. The mean effect of the protein levels and carbon source was significantly reported, whereas the highest significant THB count was recorded with 300 dietary protein and using WF as a carbon source. The growth performances of L. vannamei fed with biofloc treatments were significantly (p < 0.05) higher than the C(450) group. The highest final weight and weight gain were recorded in SB(350) treatment. The feed conversion ratio was not affected by reducing dietary protein levels; meanwhile, the protein efficiency ratio increased significantly in biofloc treatments than in the control. Overall, the results demonstrate that, compared to the control treatment of 450 dietary protein, the biofloc treatments using WF as a carbon source could compensate for the reduction in the dietary protein levels in the diet of L. vannamei and maintain higher zootechnical performance.
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spelling pubmed-92281492022-06-25 The Optimization of Dietary Protein Level and Carbon Sources on Biofloc Nutritive Values, Bacterial Abundance, and Growth Performances of Whiteleg Shrimp (Litopenaeus vannamei) Juveniles Mansour, Abdallah Tageldein Ashry, Ola A. Ashour, Mohamed Alsaqufi, Ahmed Saud Ramadan, Khaled M. A. Sharawy, Zaki Z. Life (Basel) Article A biofloc technology-based 75-day indoor growth trial in an 80 L glass aquaria was conducted to evaluate the effects of two different carbon sources (sugarcane bagasse, SB, and wheat flour, WF) on the biofloc composition, bacterial abundance, and growth of whiteleg shrimp (Litopenaeus vannamei) juveniles (0.23 ± 0.04 g). Three different levels of dietary protein content (250, 300, and 350 g protein kg(−1) diet) and two carbon sources (SB and WF) were applied (SB(250), WF(250), SB(300), WF(300), SB(350), and WF(350), respectively), comparing to a controlled diet without biofloc and fed on a 450 g protein kg(−1) diet (C(450)). With the addition of SB and WF, water quality was in the ideal recommended ranges for L. vannamei culture. At the end of the experiment, the biofloc volume increased with increasing dietary protein levels. The nutritional value of biofloc in different treatments was influenced by dietary protein and added SB and WF. Increasing dietary protein significantly increased the protein and lipid contents of the produced biofloc. The use of WF as a carbon source significantly increased lipids and nitrogen-free extract in the biofloc. The total heterotrophic bacterial (THB) count was significantly higher (p < 0.05) in WF(300) and WF(350) than in the other treatments. The mean effect of the protein levels and carbon source was significantly reported, whereas the highest significant THB count was recorded with 300 dietary protein and using WF as a carbon source. The growth performances of L. vannamei fed with biofloc treatments were significantly (p < 0.05) higher than the C(450) group. The highest final weight and weight gain were recorded in SB(350) treatment. The feed conversion ratio was not affected by reducing dietary protein levels; meanwhile, the protein efficiency ratio increased significantly in biofloc treatments than in the control. Overall, the results demonstrate that, compared to the control treatment of 450 dietary protein, the biofloc treatments using WF as a carbon source could compensate for the reduction in the dietary protein levels in the diet of L. vannamei and maintain higher zootechnical performance. MDPI 2022-06-14 /pmc/articles/PMC9228149/ /pubmed/35743919 http://dx.doi.org/10.3390/life12060888 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mansour, Abdallah Tageldein
Ashry, Ola A.
Ashour, Mohamed
Alsaqufi, Ahmed Saud
Ramadan, Khaled M. A.
Sharawy, Zaki Z.
The Optimization of Dietary Protein Level and Carbon Sources on Biofloc Nutritive Values, Bacterial Abundance, and Growth Performances of Whiteleg Shrimp (Litopenaeus vannamei) Juveniles
title The Optimization of Dietary Protein Level and Carbon Sources on Biofloc Nutritive Values, Bacterial Abundance, and Growth Performances of Whiteleg Shrimp (Litopenaeus vannamei) Juveniles
title_full The Optimization of Dietary Protein Level and Carbon Sources on Biofloc Nutritive Values, Bacterial Abundance, and Growth Performances of Whiteleg Shrimp (Litopenaeus vannamei) Juveniles
title_fullStr The Optimization of Dietary Protein Level and Carbon Sources on Biofloc Nutritive Values, Bacterial Abundance, and Growth Performances of Whiteleg Shrimp (Litopenaeus vannamei) Juveniles
title_full_unstemmed The Optimization of Dietary Protein Level and Carbon Sources on Biofloc Nutritive Values, Bacterial Abundance, and Growth Performances of Whiteleg Shrimp (Litopenaeus vannamei) Juveniles
title_short The Optimization of Dietary Protein Level and Carbon Sources on Biofloc Nutritive Values, Bacterial Abundance, and Growth Performances of Whiteleg Shrimp (Litopenaeus vannamei) Juveniles
title_sort optimization of dietary protein level and carbon sources on biofloc nutritive values, bacterial abundance, and growth performances of whiteleg shrimp (litopenaeus vannamei) juveniles
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9228149/
https://www.ncbi.nlm.nih.gov/pubmed/35743919
http://dx.doi.org/10.3390/life12060888
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