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Transcriptome Profiling Identifies Candidate Genes Contributing to Male and Female Gamete Development in Synthetic Brassica Allohexaploids

Polyploidy plays a crucial role in plant evolution and speciation. The development of male and female gametes is essential to the reproductive capacity of polyploids, but their gene expression pattern has not been fully explored in newly established polyploids. The present study aimed to reveal a de...

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Autores principales: Ji, Chengyan, Tian, Zhaoran, Liu, Yue, Shi, Gongyao, Tian, Baoming, Chen, Weiwei, Xie, Zhengqing, Han, Xingzhou, Liang, Niannian, Wei, Fang, Wei, Xiaochun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9228180/
https://www.ncbi.nlm.nih.gov/pubmed/35736707
http://dx.doi.org/10.3390/plants11121556
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author Ji, Chengyan
Tian, Zhaoran
Liu, Yue
Shi, Gongyao
Tian, Baoming
Chen, Weiwei
Xie, Zhengqing
Han, Xingzhou
Liang, Niannian
Wei, Fang
Wei, Xiaochun
author_facet Ji, Chengyan
Tian, Zhaoran
Liu, Yue
Shi, Gongyao
Tian, Baoming
Chen, Weiwei
Xie, Zhengqing
Han, Xingzhou
Liang, Niannian
Wei, Fang
Wei, Xiaochun
author_sort Ji, Chengyan
collection PubMed
description Polyploidy plays a crucial role in plant evolution and speciation. The development of male and female gametes is essential to the reproductive capacity of polyploids, but their gene expression pattern has not been fully explored in newly established polyploids. The present study aimed to reveal a detailed atlas of gene expression for gamete development in newly synthetic Brassica allohexaploids that are not naturally existing species. Comparative transcriptome profiling between developing anthers (staged from meiosis to mature pollen) and ovules (staged from meiosis to mature embryo sac) was performed using RNA-Seq analysis. A total of 8676, 9775 and 4553 upregulated differentially expressed genes (DEGs) were identified for the development of both gametes, for male-only, and for female-only gamete development, respectively, in the synthetic Brassica allohexaploids. By combining gene ontology (GO) biological process analysis and data from the published literature, we identified 37 candidate genes for DNA double-strand break formation, synapsis and the crossover of homologous recombination during male and female meiosis and 51 candidate genes for tapetum development, sporopollenin biosynthesis and pollen wall development in male gamete development. Furthermore, 23 candidate genes for mitotic progression, nuclear positioning and cell specification and development were enriched in female gamete development. This study lays a good foundation for revealing the molecular regulation of genes related to male and female gamete development in Brassica allohexaploids and provides more resourceful genetic information on the reproductive biology of Brassica polyploid breeding.
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spelling pubmed-92281802022-06-25 Transcriptome Profiling Identifies Candidate Genes Contributing to Male and Female Gamete Development in Synthetic Brassica Allohexaploids Ji, Chengyan Tian, Zhaoran Liu, Yue Shi, Gongyao Tian, Baoming Chen, Weiwei Xie, Zhengqing Han, Xingzhou Liang, Niannian Wei, Fang Wei, Xiaochun Plants (Basel) Article Polyploidy plays a crucial role in plant evolution and speciation. The development of male and female gametes is essential to the reproductive capacity of polyploids, but their gene expression pattern has not been fully explored in newly established polyploids. The present study aimed to reveal a detailed atlas of gene expression for gamete development in newly synthetic Brassica allohexaploids that are not naturally existing species. Comparative transcriptome profiling between developing anthers (staged from meiosis to mature pollen) and ovules (staged from meiosis to mature embryo sac) was performed using RNA-Seq analysis. A total of 8676, 9775 and 4553 upregulated differentially expressed genes (DEGs) were identified for the development of both gametes, for male-only, and for female-only gamete development, respectively, in the synthetic Brassica allohexaploids. By combining gene ontology (GO) biological process analysis and data from the published literature, we identified 37 candidate genes for DNA double-strand break formation, synapsis and the crossover of homologous recombination during male and female meiosis and 51 candidate genes for tapetum development, sporopollenin biosynthesis and pollen wall development in male gamete development. Furthermore, 23 candidate genes for mitotic progression, nuclear positioning and cell specification and development were enriched in female gamete development. This study lays a good foundation for revealing the molecular regulation of genes related to male and female gamete development in Brassica allohexaploids and provides more resourceful genetic information on the reproductive biology of Brassica polyploid breeding. MDPI 2022-06-13 /pmc/articles/PMC9228180/ /pubmed/35736707 http://dx.doi.org/10.3390/plants11121556 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ji, Chengyan
Tian, Zhaoran
Liu, Yue
Shi, Gongyao
Tian, Baoming
Chen, Weiwei
Xie, Zhengqing
Han, Xingzhou
Liang, Niannian
Wei, Fang
Wei, Xiaochun
Transcriptome Profiling Identifies Candidate Genes Contributing to Male and Female Gamete Development in Synthetic Brassica Allohexaploids
title Transcriptome Profiling Identifies Candidate Genes Contributing to Male and Female Gamete Development in Synthetic Brassica Allohexaploids
title_full Transcriptome Profiling Identifies Candidate Genes Contributing to Male and Female Gamete Development in Synthetic Brassica Allohexaploids
title_fullStr Transcriptome Profiling Identifies Candidate Genes Contributing to Male and Female Gamete Development in Synthetic Brassica Allohexaploids
title_full_unstemmed Transcriptome Profiling Identifies Candidate Genes Contributing to Male and Female Gamete Development in Synthetic Brassica Allohexaploids
title_short Transcriptome Profiling Identifies Candidate Genes Contributing to Male and Female Gamete Development in Synthetic Brassica Allohexaploids
title_sort transcriptome profiling identifies candidate genes contributing to male and female gamete development in synthetic brassica allohexaploids
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9228180/
https://www.ncbi.nlm.nih.gov/pubmed/35736707
http://dx.doi.org/10.3390/plants11121556
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