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A Robust Expression and Purification Method for Production of SpCas9-GFP-MBP Fusion Protein for In Vitro Applications

Genome editing using the CRISPR/Cas9 system is one of the trendiest methodologies in the scientific community. Many genome editing approaches require recombinant Streptococcus pyogenes Cas9 (SpCas9) at some point during their application, for instance, for in vitro validation of single guide RNAs (S...

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Detalles Bibliográficos
Autores principales:	Fleitas, Andrea Luciana, Señorale, Mario, Vidal, Sabina
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2022
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9228339/ https://www.ncbi.nlm.nih.gov/pubmed/35736545 http://dx.doi.org/10.3390/mps5030044

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