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Utilization of Recombinant Baculovirus Expression System to Produce the RBD Domain of SARS-CoV-2 Spike Protein

Continuous outbreaks of viral diseases in humans facilitates a need for the rapid development of viral test kits and vaccines. These require expression systems to produce a pure and high yield of target viral proteins. We utilized a baculovirus–silkworm expression system to produce the receptor bind...

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Autores principales: Fan, Youpeng, Wei, Junhong, Wang, Wei, Li, Chunfeng, Pan, Guoqing, Keiffer, Timothy, Bao, Jialing, Zhou, Zeyang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9228350/
https://www.ncbi.nlm.nih.gov/pubmed/35745526
http://dx.doi.org/10.3390/pathogens11060672
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author Fan, Youpeng
Wei, Junhong
Wang, Wei
Li, Chunfeng
Pan, Guoqing
Keiffer, Timothy
Bao, Jialing
Zhou, Zeyang
author_facet Fan, Youpeng
Wei, Junhong
Wang, Wei
Li, Chunfeng
Pan, Guoqing
Keiffer, Timothy
Bao, Jialing
Zhou, Zeyang
author_sort Fan, Youpeng
collection PubMed
description Continuous outbreaks of viral diseases in humans facilitates a need for the rapid development of viral test kits and vaccines. These require expression systems to produce a pure and high yield of target viral proteins. We utilized a baculovirus–silkworm expression system to produce the receptor binding domain (RBD) of the SARS-CoV-2 spike protein. First, we had to develop a strategy for constructing a recombinant baculovirus for RBD expression. For this, the coding region of the Bombyx mori cypovirus (BmCPV) polyhedron was assembled with the Bombyx mori nuclear polyhedrosis virus (BmNPV) promoter. We demonstrated that the recombinant baculovirus has the ability to form polyhedrons within host silkworm cells. In addition, the encapsulated BVs are able to infect silkworms by ingestion and induce foreign protein expression. In this way, we utilized this novel system to obtain a high yield of the target foreign protein, the RBD of the SARS-CoV-2 S protein. However, the viral infection rate of our recombinant BV needs to be improved. Our study shed light on developing a highly efficient expression system for the production of antigens and subsequent immunoassays and vaccines.
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spelling pubmed-92283502022-06-25 Utilization of Recombinant Baculovirus Expression System to Produce the RBD Domain of SARS-CoV-2 Spike Protein Fan, Youpeng Wei, Junhong Wang, Wei Li, Chunfeng Pan, Guoqing Keiffer, Timothy Bao, Jialing Zhou, Zeyang Pathogens Project Report Continuous outbreaks of viral diseases in humans facilitates a need for the rapid development of viral test kits and vaccines. These require expression systems to produce a pure and high yield of target viral proteins. We utilized a baculovirus–silkworm expression system to produce the receptor binding domain (RBD) of the SARS-CoV-2 spike protein. First, we had to develop a strategy for constructing a recombinant baculovirus for RBD expression. For this, the coding region of the Bombyx mori cypovirus (BmCPV) polyhedron was assembled with the Bombyx mori nuclear polyhedrosis virus (BmNPV) promoter. We demonstrated that the recombinant baculovirus has the ability to form polyhedrons within host silkworm cells. In addition, the encapsulated BVs are able to infect silkworms by ingestion and induce foreign protein expression. In this way, we utilized this novel system to obtain a high yield of the target foreign protein, the RBD of the SARS-CoV-2 S protein. However, the viral infection rate of our recombinant BV needs to be improved. Our study shed light on developing a highly efficient expression system for the production of antigens and subsequent immunoassays and vaccines. MDPI 2022-06-10 /pmc/articles/PMC9228350/ /pubmed/35745526 http://dx.doi.org/10.3390/pathogens11060672 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Project Report
Fan, Youpeng
Wei, Junhong
Wang, Wei
Li, Chunfeng
Pan, Guoqing
Keiffer, Timothy
Bao, Jialing
Zhou, Zeyang
Utilization of Recombinant Baculovirus Expression System to Produce the RBD Domain of SARS-CoV-2 Spike Protein
title Utilization of Recombinant Baculovirus Expression System to Produce the RBD Domain of SARS-CoV-2 Spike Protein
title_full Utilization of Recombinant Baculovirus Expression System to Produce the RBD Domain of SARS-CoV-2 Spike Protein
title_fullStr Utilization of Recombinant Baculovirus Expression System to Produce the RBD Domain of SARS-CoV-2 Spike Protein
title_full_unstemmed Utilization of Recombinant Baculovirus Expression System to Produce the RBD Domain of SARS-CoV-2 Spike Protein
title_short Utilization of Recombinant Baculovirus Expression System to Produce the RBD Domain of SARS-CoV-2 Spike Protein
title_sort utilization of recombinant baculovirus expression system to produce the rbd domain of sars-cov-2 spike protein
topic Project Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9228350/
https://www.ncbi.nlm.nih.gov/pubmed/35745526
http://dx.doi.org/10.3390/pathogens11060672
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