Cargando…

Encapsulation of Human-Bone-Marrow-Derived Mesenchymal Stem Cells in Small Alginate Beads Using One-Step Emulsification by Internal Gelation: In Vitro, and In Vivo Evaluation in Degenerate Intervertebral Disc Model

Cell microencapsulation in gel beads contributes to many biomedical processes and pharmaceutical applications. Small beads (<300 µm) offer distinct advantages, mainly due to improved mass transfer and mechanical strength. Here, we describe, for the first time, the encapsulation of human-bone-marr...

Descripción completa

Detalles Bibliográficos
Autores principales: Sivan, Sarit S., Bonstein, Iris, Marmor, Yariv N., Pelled, Gadi, Gazit, Zulma, Amit, Michal
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9228465/
https://www.ncbi.nlm.nih.gov/pubmed/35745752
http://dx.doi.org/10.3390/pharmaceutics14061179
_version_ 1784734484712652800
author Sivan, Sarit S.
Bonstein, Iris
Marmor, Yariv N.
Pelled, Gadi
Gazit, Zulma
Amit, Michal
author_facet Sivan, Sarit S.
Bonstein, Iris
Marmor, Yariv N.
Pelled, Gadi
Gazit, Zulma
Amit, Michal
author_sort Sivan, Sarit S.
collection PubMed
description Cell microencapsulation in gel beads contributes to many biomedical processes and pharmaceutical applications. Small beads (<300 µm) offer distinct advantages, mainly due to improved mass transfer and mechanical strength. Here, we describe, for the first time, the encapsulation of human-bone-marrow-derived mesenchymal stem cells (hBM-MSCs) in small-sized microspheres, using one-step emulsification by internal gelation. Small (127–257 µm) high-mannuronic-alginate microspheres were prepared at high agitation rates (800–1000 rpm), enabling control over the bead size and shape. The average viability of encapsulated hBM-MSCs after 2 weeks was 81 ± 4.3% for the higher agitation rates. hBM-MSC-loaded microspheres seeded within a glycosaminoglycan (GAG) analogue, which was previously proposed as a mechanically equivalent implant for degenerate discs, kept their viability, sphericity, and integrity for at least 6 weeks. A preliminary in vivo study of hBM-MSC-loaded microspheres implanted (via a GAG-analogue hydrogel) in a rat injured intervertebral disc model demonstrated long-lasting viability and biocompatibility for at least 8 weeks post-implantation. The proposed method offers an effective and reproducible way to maintain long-lasting viability in vitro and in vivo. This approach not only utilizes the benefits of a simple, mild, and scalable method, but also allows for the easy control of the bead size and shape by the agitation rate, which, overall, makes it a very attractive platform for regenerative-medicine applications.
format Online
Article
Text
id pubmed-9228465
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-92284652022-06-25 Encapsulation of Human-Bone-Marrow-Derived Mesenchymal Stem Cells in Small Alginate Beads Using One-Step Emulsification by Internal Gelation: In Vitro, and In Vivo Evaluation in Degenerate Intervertebral Disc Model Sivan, Sarit S. Bonstein, Iris Marmor, Yariv N. Pelled, Gadi Gazit, Zulma Amit, Michal Pharmaceutics Article Cell microencapsulation in gel beads contributes to many biomedical processes and pharmaceutical applications. Small beads (<300 µm) offer distinct advantages, mainly due to improved mass transfer and mechanical strength. Here, we describe, for the first time, the encapsulation of human-bone-marrow-derived mesenchymal stem cells (hBM-MSCs) in small-sized microspheres, using one-step emulsification by internal gelation. Small (127–257 µm) high-mannuronic-alginate microspheres were prepared at high agitation rates (800–1000 rpm), enabling control over the bead size and shape. The average viability of encapsulated hBM-MSCs after 2 weeks was 81 ± 4.3% for the higher agitation rates. hBM-MSC-loaded microspheres seeded within a glycosaminoglycan (GAG) analogue, which was previously proposed as a mechanically equivalent implant for degenerate discs, kept their viability, sphericity, and integrity for at least 6 weeks. A preliminary in vivo study of hBM-MSC-loaded microspheres implanted (via a GAG-analogue hydrogel) in a rat injured intervertebral disc model demonstrated long-lasting viability and biocompatibility for at least 8 weeks post-implantation. The proposed method offers an effective and reproducible way to maintain long-lasting viability in vitro and in vivo. This approach not only utilizes the benefits of a simple, mild, and scalable method, but also allows for the easy control of the bead size and shape by the agitation rate, which, overall, makes it a very attractive platform for regenerative-medicine applications. MDPI 2022-05-31 /pmc/articles/PMC9228465/ /pubmed/35745752 http://dx.doi.org/10.3390/pharmaceutics14061179 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sivan, Sarit S.
Bonstein, Iris
Marmor, Yariv N.
Pelled, Gadi
Gazit, Zulma
Amit, Michal
Encapsulation of Human-Bone-Marrow-Derived Mesenchymal Stem Cells in Small Alginate Beads Using One-Step Emulsification by Internal Gelation: In Vitro, and In Vivo Evaluation in Degenerate Intervertebral Disc Model
title Encapsulation of Human-Bone-Marrow-Derived Mesenchymal Stem Cells in Small Alginate Beads Using One-Step Emulsification by Internal Gelation: In Vitro, and In Vivo Evaluation in Degenerate Intervertebral Disc Model
title_full Encapsulation of Human-Bone-Marrow-Derived Mesenchymal Stem Cells in Small Alginate Beads Using One-Step Emulsification by Internal Gelation: In Vitro, and In Vivo Evaluation in Degenerate Intervertebral Disc Model
title_fullStr Encapsulation of Human-Bone-Marrow-Derived Mesenchymal Stem Cells in Small Alginate Beads Using One-Step Emulsification by Internal Gelation: In Vitro, and In Vivo Evaluation in Degenerate Intervertebral Disc Model
title_full_unstemmed Encapsulation of Human-Bone-Marrow-Derived Mesenchymal Stem Cells in Small Alginate Beads Using One-Step Emulsification by Internal Gelation: In Vitro, and In Vivo Evaluation in Degenerate Intervertebral Disc Model
title_short Encapsulation of Human-Bone-Marrow-Derived Mesenchymal Stem Cells in Small Alginate Beads Using One-Step Emulsification by Internal Gelation: In Vitro, and In Vivo Evaluation in Degenerate Intervertebral Disc Model
title_sort encapsulation of human-bone-marrow-derived mesenchymal stem cells in small alginate beads using one-step emulsification by internal gelation: in vitro, and in vivo evaluation in degenerate intervertebral disc model
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9228465/
https://www.ncbi.nlm.nih.gov/pubmed/35745752
http://dx.doi.org/10.3390/pharmaceutics14061179
work_keys_str_mv AT sivansarits encapsulationofhumanbonemarrowderivedmesenchymalstemcellsinsmallalginatebeadsusingonestepemulsificationbyinternalgelationinvitroandinvivoevaluationindegenerateintervertebraldiscmodel
AT bonsteiniris encapsulationofhumanbonemarrowderivedmesenchymalstemcellsinsmallalginatebeadsusingonestepemulsificationbyinternalgelationinvitroandinvivoevaluationindegenerateintervertebraldiscmodel
AT marmoryarivn encapsulationofhumanbonemarrowderivedmesenchymalstemcellsinsmallalginatebeadsusingonestepemulsificationbyinternalgelationinvitroandinvivoevaluationindegenerateintervertebraldiscmodel
AT pelledgadi encapsulationofhumanbonemarrowderivedmesenchymalstemcellsinsmallalginatebeadsusingonestepemulsificationbyinternalgelationinvitroandinvivoevaluationindegenerateintervertebraldiscmodel
AT gazitzulma encapsulationofhumanbonemarrowderivedmesenchymalstemcellsinsmallalginatebeadsusingonestepemulsificationbyinternalgelationinvitroandinvivoevaluationindegenerateintervertebraldiscmodel
AT amitmichal encapsulationofhumanbonemarrowderivedmesenchymalstemcellsinsmallalginatebeadsusingonestepemulsificationbyinternalgelationinvitroandinvivoevaluationindegenerateintervertebraldiscmodel