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The Effect of Encapsulated Apigenin Nanoparticles on HePG-2 Cells through Regulation of P53
Apigenin (Ap) is one of the most important natural flavonoids that has potent anticancer activity. This study was designed, for the first time, to load Ap into chitosan to improve its hydrophobicity and then it was coated with albumin-folic acid to increase its stability and bioavailability and to t...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9228521/ https://www.ncbi.nlm.nih.gov/pubmed/35745733 http://dx.doi.org/10.3390/pharmaceutics14061160 |
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author | Mabrouk Zayed, Mayada Mohamed Sahyon, Heba A. Hanafy, Nemany A. N. El-Kemary, Maged A. |
author_facet | Mabrouk Zayed, Mayada Mohamed Sahyon, Heba A. Hanafy, Nemany A. N. El-Kemary, Maged A. |
author_sort | Mabrouk Zayed, Mayada Mohamed |
collection | PubMed |
description | Apigenin (Ap) is one of the most important natural flavonoids that has potent anticancer activity. This study was designed, for the first time, to load Ap into chitosan to improve its hydrophobicity and then it was coated with albumin-folic acid to increase its stability and bioavailability and to target cancer cells. The newly developed encapsulated Ap (Ap-CH-BSA-FANPs) was characterized and tested in vitro. The zeta potential of −17.0 mV was within the recommended range (−30 mV to +30 mV), indicating that encapsulated apigenin would not quickly settle and would be suspended. The in vitro results proved the great anticancer activity of the encapsulated apigenin on HePG-2 cells compared to pure Ap. The treated HePG-2 cells with Ap-CH-BSA-FANPs demonstrated the induction of apoptosis by increasing p53 gene expression, arresting the cell cycle, increasing caspase-9 levels, and decreasing both the MMP9 gene and Bcl-2 protein expression levels. Moreover, the higher antioxidant activity of the encapsulated apigenin treatment was evident through increasing SOD levels and decreasing the CAT concentration. In conclusion, the Ap-CH-BSA-FANPs were easy to produce with low coast, continued drug release, good loading capacity, high solubility in physiological pH, and were more stable than the formerly Ap-loaded liposomes or PLGA. Moreover, Ap-CH-BSA-FANPs may be a promising chemotherapeutic agent in the treatment of HCC. |
format | Online Article Text |
id | pubmed-9228521 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-92285212022-06-25 The Effect of Encapsulated Apigenin Nanoparticles on HePG-2 Cells through Regulation of P53 Mabrouk Zayed, Mayada Mohamed Sahyon, Heba A. Hanafy, Nemany A. N. El-Kemary, Maged A. Pharmaceutics Article Apigenin (Ap) is one of the most important natural flavonoids that has potent anticancer activity. This study was designed, for the first time, to load Ap into chitosan to improve its hydrophobicity and then it was coated with albumin-folic acid to increase its stability and bioavailability and to target cancer cells. The newly developed encapsulated Ap (Ap-CH-BSA-FANPs) was characterized and tested in vitro. The zeta potential of −17.0 mV was within the recommended range (−30 mV to +30 mV), indicating that encapsulated apigenin would not quickly settle and would be suspended. The in vitro results proved the great anticancer activity of the encapsulated apigenin on HePG-2 cells compared to pure Ap. The treated HePG-2 cells with Ap-CH-BSA-FANPs demonstrated the induction of apoptosis by increasing p53 gene expression, arresting the cell cycle, increasing caspase-9 levels, and decreasing both the MMP9 gene and Bcl-2 protein expression levels. Moreover, the higher antioxidant activity of the encapsulated apigenin treatment was evident through increasing SOD levels and decreasing the CAT concentration. In conclusion, the Ap-CH-BSA-FANPs were easy to produce with low coast, continued drug release, good loading capacity, high solubility in physiological pH, and were more stable than the formerly Ap-loaded liposomes or PLGA. Moreover, Ap-CH-BSA-FANPs may be a promising chemotherapeutic agent in the treatment of HCC. MDPI 2022-05-29 /pmc/articles/PMC9228521/ /pubmed/35745733 http://dx.doi.org/10.3390/pharmaceutics14061160 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Mabrouk Zayed, Mayada Mohamed Sahyon, Heba A. Hanafy, Nemany A. N. El-Kemary, Maged A. The Effect of Encapsulated Apigenin Nanoparticles on HePG-2 Cells through Regulation of P53 |
title | The Effect of Encapsulated Apigenin Nanoparticles on HePG-2 Cells through Regulation of P53 |
title_full | The Effect of Encapsulated Apigenin Nanoparticles on HePG-2 Cells through Regulation of P53 |
title_fullStr | The Effect of Encapsulated Apigenin Nanoparticles on HePG-2 Cells through Regulation of P53 |
title_full_unstemmed | The Effect of Encapsulated Apigenin Nanoparticles on HePG-2 Cells through Regulation of P53 |
title_short | The Effect of Encapsulated Apigenin Nanoparticles on HePG-2 Cells through Regulation of P53 |
title_sort | effect of encapsulated apigenin nanoparticles on hepg-2 cells through regulation of p53 |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9228521/ https://www.ncbi.nlm.nih.gov/pubmed/35745733 http://dx.doi.org/10.3390/pharmaceutics14061160 |
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