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Mycobacterium chimaera Identification Using MALDI-TOF MS Technology: A Practical Approach for the Clinical Microbiology Laboratories

Mycobacterium chimaera (MC) is an environmental, slowly growing, non-tuberculous mycobacterium (NTM) belonging to Mycobacterium avium complex (MAC), which recently has been linked to severe cardiovascular infections following open heart and vascular surgery. The majority of the diagnostic laboratory...

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Autores principales: Bagnarino, Jessica, Barbarini, Daniela, Russello, Giuseppe, Siciliano, Mariangela, Monzillo, Vincenzina, Baldanti, Fausto, Carretto, Edoardo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9228860/
https://www.ncbi.nlm.nih.gov/pubmed/35744702
http://dx.doi.org/10.3390/microorganisms10061184
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author Bagnarino, Jessica
Barbarini, Daniela
Russello, Giuseppe
Siciliano, Mariangela
Monzillo, Vincenzina
Baldanti, Fausto
Carretto, Edoardo
author_facet Bagnarino, Jessica
Barbarini, Daniela
Russello, Giuseppe
Siciliano, Mariangela
Monzillo, Vincenzina
Baldanti, Fausto
Carretto, Edoardo
author_sort Bagnarino, Jessica
collection PubMed
description Mycobacterium chimaera (MC) is an environmental, slowly growing, non-tuberculous mycobacterium (NTM) belonging to Mycobacterium avium complex (MAC), which recently has been linked to severe cardiovascular infections following open heart and vascular surgery. The majority of the diagnostic laboratory tests used in routine are not able to distinguish MC from M. intracellulare (MI), because of the great genetic similarity existing between these two species. The Genotype Mycobacterium NTM-DR™ represents a valid method to differentiate between these species, but it is expensive, requiring also specialized personnel. Recently, MALDI-TOF MS has been proposed to identify relevant NTM. However, a software implementation is required to distinguish between MC and MI, presenting the two microorganisms’ overlapping spectra. The present study evaluates the feasibility of applying a MALDI-TOF logarithmic-based analysis in the routine of a clinical microbiology laboratory, and proposes an easy-to-use template spreadsheet to make the results quickly interpretable. The protocol was previously validated through the identification of 87 strains of MC/MI collected from clinical and environmental samples, and it was identified using the GenoType Mycobacterium NTM-DR™ and/or WGS. The proposed protocol provides accurate identification for the isolates tested; moreover, it is less expensive and more rapid than sequencing methods and can be implemented with minimum effort.
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spelling pubmed-92288602022-06-25 Mycobacterium chimaera Identification Using MALDI-TOF MS Technology: A Practical Approach for the Clinical Microbiology Laboratories Bagnarino, Jessica Barbarini, Daniela Russello, Giuseppe Siciliano, Mariangela Monzillo, Vincenzina Baldanti, Fausto Carretto, Edoardo Microorganisms Article Mycobacterium chimaera (MC) is an environmental, slowly growing, non-tuberculous mycobacterium (NTM) belonging to Mycobacterium avium complex (MAC), which recently has been linked to severe cardiovascular infections following open heart and vascular surgery. The majority of the diagnostic laboratory tests used in routine are not able to distinguish MC from M. intracellulare (MI), because of the great genetic similarity existing between these two species. The Genotype Mycobacterium NTM-DR™ represents a valid method to differentiate between these species, but it is expensive, requiring also specialized personnel. Recently, MALDI-TOF MS has been proposed to identify relevant NTM. However, a software implementation is required to distinguish between MC and MI, presenting the two microorganisms’ overlapping spectra. The present study evaluates the feasibility of applying a MALDI-TOF logarithmic-based analysis in the routine of a clinical microbiology laboratory, and proposes an easy-to-use template spreadsheet to make the results quickly interpretable. The protocol was previously validated through the identification of 87 strains of MC/MI collected from clinical and environmental samples, and it was identified using the GenoType Mycobacterium NTM-DR™ and/or WGS. The proposed protocol provides accurate identification for the isolates tested; moreover, it is less expensive and more rapid than sequencing methods and can be implemented with minimum effort. MDPI 2022-06-09 /pmc/articles/PMC9228860/ /pubmed/35744702 http://dx.doi.org/10.3390/microorganisms10061184 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bagnarino, Jessica
Barbarini, Daniela
Russello, Giuseppe
Siciliano, Mariangela
Monzillo, Vincenzina
Baldanti, Fausto
Carretto, Edoardo
Mycobacterium chimaera Identification Using MALDI-TOF MS Technology: A Practical Approach for the Clinical Microbiology Laboratories
title Mycobacterium chimaera Identification Using MALDI-TOF MS Technology: A Practical Approach for the Clinical Microbiology Laboratories
title_full Mycobacterium chimaera Identification Using MALDI-TOF MS Technology: A Practical Approach for the Clinical Microbiology Laboratories
title_fullStr Mycobacterium chimaera Identification Using MALDI-TOF MS Technology: A Practical Approach for the Clinical Microbiology Laboratories
title_full_unstemmed Mycobacterium chimaera Identification Using MALDI-TOF MS Technology: A Practical Approach for the Clinical Microbiology Laboratories
title_short Mycobacterium chimaera Identification Using MALDI-TOF MS Technology: A Practical Approach for the Clinical Microbiology Laboratories
title_sort mycobacterium chimaera identification using maldi-tof ms technology: a practical approach for the clinical microbiology laboratories
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9228860/
https://www.ncbi.nlm.nih.gov/pubmed/35744702
http://dx.doi.org/10.3390/microorganisms10061184
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