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The M13 Phage Assembly Machine Has a Membrane-Spanning Oligomeric Ring Structure

Bacteriophage M13 assembles its progeny particles in the inner membrane of the host. The major component of the assembly machine is G1p and together with G11p it generates an oligomeric structure with a pore-like inner cavity and an ATP hydrolysing domain. This allows the formation of the phage fila...

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Autores principales: Haase, Maximilian, Tessmer, Lutz, Köhnlechner, Lilian, Kuhn, Andreas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9228878/
https://www.ncbi.nlm.nih.gov/pubmed/35746635
http://dx.doi.org/10.3390/v14061163
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author Haase, Maximilian
Tessmer, Lutz
Köhnlechner, Lilian
Kuhn, Andreas
author_facet Haase, Maximilian
Tessmer, Lutz
Köhnlechner, Lilian
Kuhn, Andreas
author_sort Haase, Maximilian
collection PubMed
description Bacteriophage M13 assembles its progeny particles in the inner membrane of the host. The major component of the assembly machine is G1p and together with G11p it generates an oligomeric structure with a pore-like inner cavity and an ATP hydrolysing domain. This allows the formation of the phage filament, which assembles multiple copies of the membrane-inserted major coat protein G8p around the extruding single-stranded circular DNA. The phage filament then passes through the G4p secretin that is localized in the outer membrane. Presumably, the inner membrane G1p/G11p and the outer G4p form a common complex. To unravel the structural details of the M13 assembly machine, we purified G1p from infected E. coli cells. The protein was overproduced together with G11p and solubilized from the membrane as a multimeric complex with a size of about 320 kDa. The complex revealed a pore-like structure with an outer diameter of about 12 nm, matching the dimensions of the outer membrane G4p secretin. The function of the M13 assembly machine for phage generation and secretion is discussed.
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spelling pubmed-92288782022-06-25 The M13 Phage Assembly Machine Has a Membrane-Spanning Oligomeric Ring Structure Haase, Maximilian Tessmer, Lutz Köhnlechner, Lilian Kuhn, Andreas Viruses Article Bacteriophage M13 assembles its progeny particles in the inner membrane of the host. The major component of the assembly machine is G1p and together with G11p it generates an oligomeric structure with a pore-like inner cavity and an ATP hydrolysing domain. This allows the formation of the phage filament, which assembles multiple copies of the membrane-inserted major coat protein G8p around the extruding single-stranded circular DNA. The phage filament then passes through the G4p secretin that is localized in the outer membrane. Presumably, the inner membrane G1p/G11p and the outer G4p form a common complex. To unravel the structural details of the M13 assembly machine, we purified G1p from infected E. coli cells. The protein was overproduced together with G11p and solubilized from the membrane as a multimeric complex with a size of about 320 kDa. The complex revealed a pore-like structure with an outer diameter of about 12 nm, matching the dimensions of the outer membrane G4p secretin. The function of the M13 assembly machine for phage generation and secretion is discussed. MDPI 2022-05-27 /pmc/articles/PMC9228878/ /pubmed/35746635 http://dx.doi.org/10.3390/v14061163 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Haase, Maximilian
Tessmer, Lutz
Köhnlechner, Lilian
Kuhn, Andreas
The M13 Phage Assembly Machine Has a Membrane-Spanning Oligomeric Ring Structure
title The M13 Phage Assembly Machine Has a Membrane-Spanning Oligomeric Ring Structure
title_full The M13 Phage Assembly Machine Has a Membrane-Spanning Oligomeric Ring Structure
title_fullStr The M13 Phage Assembly Machine Has a Membrane-Spanning Oligomeric Ring Structure
title_full_unstemmed The M13 Phage Assembly Machine Has a Membrane-Spanning Oligomeric Ring Structure
title_short The M13 Phage Assembly Machine Has a Membrane-Spanning Oligomeric Ring Structure
title_sort m13 phage assembly machine has a membrane-spanning oligomeric ring structure
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9228878/
https://www.ncbi.nlm.nih.gov/pubmed/35746635
http://dx.doi.org/10.3390/v14061163
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