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MALDI Mass Spectrometry Imaging Highlights Specific Metabolome and Lipidome Profiles in Salivary Gland Tumor Tissues

Salivary gland tumors are relatively uncommon neoplasms that represent less than 5% of head and neck tumors, and about 90% are in the parotid gland. The wide variety of histologies and tumor characteristics makes diagnosis and treatment challenging. In the present study, Matrix-assisted laser desorp...

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Autores principales: Sommella, Eduardo, Salviati, Emanuela, Caponigro, Vicky, Grimaldi, Manuela, Musella, Simona, Bertamino, Alessia, Cacace, Luigi, Palladino, Remo, Mauro, Giuseppe Di, Marini, Federico, D’Ursi, Anna Maria, Campiglia, Pietro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9228942/
https://www.ncbi.nlm.nih.gov/pubmed/35736462
http://dx.doi.org/10.3390/metabo12060530
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author Sommella, Eduardo
Salviati, Emanuela
Caponigro, Vicky
Grimaldi, Manuela
Musella, Simona
Bertamino, Alessia
Cacace, Luigi
Palladino, Remo
Mauro, Giuseppe Di
Marini, Federico
D’Ursi, Anna Maria
Campiglia, Pietro
author_facet Sommella, Eduardo
Salviati, Emanuela
Caponigro, Vicky
Grimaldi, Manuela
Musella, Simona
Bertamino, Alessia
Cacace, Luigi
Palladino, Remo
Mauro, Giuseppe Di
Marini, Federico
D’Ursi, Anna Maria
Campiglia, Pietro
author_sort Sommella, Eduardo
collection PubMed
description Salivary gland tumors are relatively uncommon neoplasms that represent less than 5% of head and neck tumors, and about 90% are in the parotid gland. The wide variety of histologies and tumor characteristics makes diagnosis and treatment challenging. In the present study, Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) was used to discriminate the pathological regions of patient-derived biopsies of parotid neoplasms by metabolomic and lipidomic profiles. Fresh frozen parotid tissues were analyzed by MALDI time-of-flight (TOF) MSI, both in positive and negative ionization modes, and additional MALDI-Fourier-transform ion cyclotron resonance (FT-ICR) MSI was carried out for metabolite annotation. MALDI-TOF-MSI spatial segmentation maps with different molecular signatures were compared with the histologic annotation. To maximize the information related to specific alterations between the pathological and healthy tissues, unsupervised (principal component analysis, PCA) and supervised (partial least squares-discriminant analysis, PLS-DA) multivariate analyses were performed presenting a 95.00% accuracy in cross-validation. Glycerophospholipids significantly increased in tumor tissues, while sphingomyelins and triacylglycerols, key players in the signaling pathway and energy production, were sensibly reduced. In addition, a significant increase of amino acids and nucleotide intermediates, consistent with the bioenergetics request of tumor cells, was observed. These results underline the potential of MALDI-MSI as a complementary diagnostic tool to improve the specificity of diagnosis and monitoring of pharmacological therapies.
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spelling pubmed-92289422022-06-25 MALDI Mass Spectrometry Imaging Highlights Specific Metabolome and Lipidome Profiles in Salivary Gland Tumor Tissues Sommella, Eduardo Salviati, Emanuela Caponigro, Vicky Grimaldi, Manuela Musella, Simona Bertamino, Alessia Cacace, Luigi Palladino, Remo Mauro, Giuseppe Di Marini, Federico D’Ursi, Anna Maria Campiglia, Pietro Metabolites Article Salivary gland tumors are relatively uncommon neoplasms that represent less than 5% of head and neck tumors, and about 90% are in the parotid gland. The wide variety of histologies and tumor characteristics makes diagnosis and treatment challenging. In the present study, Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) was used to discriminate the pathological regions of patient-derived biopsies of parotid neoplasms by metabolomic and lipidomic profiles. Fresh frozen parotid tissues were analyzed by MALDI time-of-flight (TOF) MSI, both in positive and negative ionization modes, and additional MALDI-Fourier-transform ion cyclotron resonance (FT-ICR) MSI was carried out for metabolite annotation. MALDI-TOF-MSI spatial segmentation maps with different molecular signatures were compared with the histologic annotation. To maximize the information related to specific alterations between the pathological and healthy tissues, unsupervised (principal component analysis, PCA) and supervised (partial least squares-discriminant analysis, PLS-DA) multivariate analyses were performed presenting a 95.00% accuracy in cross-validation. Glycerophospholipids significantly increased in tumor tissues, while sphingomyelins and triacylglycerols, key players in the signaling pathway and energy production, were sensibly reduced. In addition, a significant increase of amino acids and nucleotide intermediates, consistent with the bioenergetics request of tumor cells, was observed. These results underline the potential of MALDI-MSI as a complementary diagnostic tool to improve the specificity of diagnosis and monitoring of pharmacological therapies. MDPI 2022-06-08 /pmc/articles/PMC9228942/ /pubmed/35736462 http://dx.doi.org/10.3390/metabo12060530 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sommella, Eduardo
Salviati, Emanuela
Caponigro, Vicky
Grimaldi, Manuela
Musella, Simona
Bertamino, Alessia
Cacace, Luigi
Palladino, Remo
Mauro, Giuseppe Di
Marini, Federico
D’Ursi, Anna Maria
Campiglia, Pietro
MALDI Mass Spectrometry Imaging Highlights Specific Metabolome and Lipidome Profiles in Salivary Gland Tumor Tissues
title MALDI Mass Spectrometry Imaging Highlights Specific Metabolome and Lipidome Profiles in Salivary Gland Tumor Tissues
title_full MALDI Mass Spectrometry Imaging Highlights Specific Metabolome and Lipidome Profiles in Salivary Gland Tumor Tissues
title_fullStr MALDI Mass Spectrometry Imaging Highlights Specific Metabolome and Lipidome Profiles in Salivary Gland Tumor Tissues
title_full_unstemmed MALDI Mass Spectrometry Imaging Highlights Specific Metabolome and Lipidome Profiles in Salivary Gland Tumor Tissues
title_short MALDI Mass Spectrometry Imaging Highlights Specific Metabolome and Lipidome Profiles in Salivary Gland Tumor Tissues
title_sort maldi mass spectrometry imaging highlights specific metabolome and lipidome profiles in salivary gland tumor tissues
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9228942/
https://www.ncbi.nlm.nih.gov/pubmed/35736462
http://dx.doi.org/10.3390/metabo12060530
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