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Enhancing Biocide Efficacy: Targeting Extracellular DNA for Marine Biofilm Disruption

Biofilm formation is a global health, safety and economic concern. The extracellular composition of deleterious multispecies biofilms remains uncanvassed, leading to an absence of targeted biofilm mitigation strategies. Besides economic incentives, drive also exists from industry and research to dev...

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Autores principales: Tuck, Benjamin, Watkin, Elizabeth, Somers, Anthony, Forsyth, Maria, Machuca, Laura L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9228965/
https://www.ncbi.nlm.nih.gov/pubmed/35744744
http://dx.doi.org/10.3390/microorganisms10061227
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author Tuck, Benjamin
Watkin, Elizabeth
Somers, Anthony
Forsyth, Maria
Machuca, Laura L.
author_facet Tuck, Benjamin
Watkin, Elizabeth
Somers, Anthony
Forsyth, Maria
Machuca, Laura L.
author_sort Tuck, Benjamin
collection PubMed
description Biofilm formation is a global health, safety and economic concern. The extracellular composition of deleterious multispecies biofilms remains uncanvassed, leading to an absence of targeted biofilm mitigation strategies. Besides economic incentives, drive also exists from industry and research to develop and apply environmentally sustainable chemical treatments (biocides); especially in engineered systems associated with the marine environment. Recently, extracellular DNA (eDNA) was implicated as a critical structural polymer in marine biofilms. Additionally, an environmentally sustainable, multi-functional biocide was also introduced to manage corrosion and biofilm formation. To anticipate biofilm tolerance acquisition to chemical treatments and reduce biocide application quantities, the present research investigated eDNA as a target for biofilm dispersal and potential enhancement of biocide function. Results indicate that mature biofilm viability can be reduced by two-fold using reduced concentrations of the biocide alone (1 mM instead of the recommended 10 mM). Importantly, through the incorporation of an eDNA degradation stage, biocide function could be enhanced by a further ~90% (one further log reduction in viability). Biofilm architecture analysis post-treatment revealed that endonuclease targeting of the matrix allowed greater biocide penetration, leading to the observed viability reduction. Biofilm matrix eDNA is a promising target for biofilm dispersal and antimicrobial enhancement in clinical and engineered systems.
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spelling pubmed-92289652022-06-25 Enhancing Biocide Efficacy: Targeting Extracellular DNA for Marine Biofilm Disruption Tuck, Benjamin Watkin, Elizabeth Somers, Anthony Forsyth, Maria Machuca, Laura L. Microorganisms Article Biofilm formation is a global health, safety and economic concern. The extracellular composition of deleterious multispecies biofilms remains uncanvassed, leading to an absence of targeted biofilm mitigation strategies. Besides economic incentives, drive also exists from industry and research to develop and apply environmentally sustainable chemical treatments (biocides); especially in engineered systems associated with the marine environment. Recently, extracellular DNA (eDNA) was implicated as a critical structural polymer in marine biofilms. Additionally, an environmentally sustainable, multi-functional biocide was also introduced to manage corrosion and biofilm formation. To anticipate biofilm tolerance acquisition to chemical treatments and reduce biocide application quantities, the present research investigated eDNA as a target for biofilm dispersal and potential enhancement of biocide function. Results indicate that mature biofilm viability can be reduced by two-fold using reduced concentrations of the biocide alone (1 mM instead of the recommended 10 mM). Importantly, through the incorporation of an eDNA degradation stage, biocide function could be enhanced by a further ~90% (one further log reduction in viability). Biofilm architecture analysis post-treatment revealed that endonuclease targeting of the matrix allowed greater biocide penetration, leading to the observed viability reduction. Biofilm matrix eDNA is a promising target for biofilm dispersal and antimicrobial enhancement in clinical and engineered systems. MDPI 2022-06-15 /pmc/articles/PMC9228965/ /pubmed/35744744 http://dx.doi.org/10.3390/microorganisms10061227 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tuck, Benjamin
Watkin, Elizabeth
Somers, Anthony
Forsyth, Maria
Machuca, Laura L.
Enhancing Biocide Efficacy: Targeting Extracellular DNA for Marine Biofilm Disruption
title Enhancing Biocide Efficacy: Targeting Extracellular DNA for Marine Biofilm Disruption
title_full Enhancing Biocide Efficacy: Targeting Extracellular DNA for Marine Biofilm Disruption
title_fullStr Enhancing Biocide Efficacy: Targeting Extracellular DNA for Marine Biofilm Disruption
title_full_unstemmed Enhancing Biocide Efficacy: Targeting Extracellular DNA for Marine Biofilm Disruption
title_short Enhancing Biocide Efficacy: Targeting Extracellular DNA for Marine Biofilm Disruption
title_sort enhancing biocide efficacy: targeting extracellular dna for marine biofilm disruption
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9228965/
https://www.ncbi.nlm.nih.gov/pubmed/35744744
http://dx.doi.org/10.3390/microorganisms10061227
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