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In Vitro and In Vivo Characterization of a New Strain of Mosquito Flavivirus Derived from Culicoides
Mosquito-specific flaviviruses comprise a group of insect-specific viruses with a single positive RNA, which can affect the duplication of mosquito-borne viruses and the life growth of mosquitoes, and which have the potential to be developed as a vaccine platform for mosquito-borne viruses. In this...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9229015/ https://www.ncbi.nlm.nih.gov/pubmed/35746769 http://dx.doi.org/10.3390/v14061298 |
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author | Huang, Yi Zhang, Hongqing Li, Xiaodan Zhao, Lu Cai, Dirui Wang, Shunlong Ren, Nanjie Ma, Haixia Huang, Doudou Wang, Fei Yuan, Zhiming Zhang, Bo Xia, Han |
author_facet | Huang, Yi Zhang, Hongqing Li, Xiaodan Zhao, Lu Cai, Dirui Wang, Shunlong Ren, Nanjie Ma, Haixia Huang, Doudou Wang, Fei Yuan, Zhiming Zhang, Bo Xia, Han |
author_sort | Huang, Yi |
collection | PubMed |
description | Mosquito-specific flaviviruses comprise a group of insect-specific viruses with a single positive RNA, which can affect the duplication of mosquito-borne viruses and the life growth of mosquitoes, and which have the potential to be developed as a vaccine platform for mosquito-borne viruses. In this study, a strain of mosquito flavivirus (MFV) YN15-283-02 was detected in Culicoides collected from Yunnan, China. The isolation of the purified MFV YN15-283-02 from cell culture failed, and the virus was then rescued by an infectious clone. To study the biological features of MFV YN15-283-02 in vitro and in vivo, electron microscopy, phylogenetic tree, and viral growth kinetic analyses were performed in both cell lines and mosquitoes. The rescued MFV (rMFV) YN15-283-02 duplicated and reached a peak in C6/36 cells at 6 d.p.i. with approximately 2 × 10(6) RNA copies/μL (RNA to cell ratio of 0.1), but without displaying a cytopathic effect. In addition, the infection rate for the rMFV in Ae. aegypti show a low level in both larvae (≤15%) and adult mosquitoes (≤12%). |
format | Online Article Text |
id | pubmed-9229015 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-92290152022-06-25 In Vitro and In Vivo Characterization of a New Strain of Mosquito Flavivirus Derived from Culicoides Huang, Yi Zhang, Hongqing Li, Xiaodan Zhao, Lu Cai, Dirui Wang, Shunlong Ren, Nanjie Ma, Haixia Huang, Doudou Wang, Fei Yuan, Zhiming Zhang, Bo Xia, Han Viruses Article Mosquito-specific flaviviruses comprise a group of insect-specific viruses with a single positive RNA, which can affect the duplication of mosquito-borne viruses and the life growth of mosquitoes, and which have the potential to be developed as a vaccine platform for mosquito-borne viruses. In this study, a strain of mosquito flavivirus (MFV) YN15-283-02 was detected in Culicoides collected from Yunnan, China. The isolation of the purified MFV YN15-283-02 from cell culture failed, and the virus was then rescued by an infectious clone. To study the biological features of MFV YN15-283-02 in vitro and in vivo, electron microscopy, phylogenetic tree, and viral growth kinetic analyses were performed in both cell lines and mosquitoes. The rescued MFV (rMFV) YN15-283-02 duplicated and reached a peak in C6/36 cells at 6 d.p.i. with approximately 2 × 10(6) RNA copies/μL (RNA to cell ratio of 0.1), but without displaying a cytopathic effect. In addition, the infection rate for the rMFV in Ae. aegypti show a low level in both larvae (≤15%) and adult mosquitoes (≤12%). MDPI 2022-06-14 /pmc/articles/PMC9229015/ /pubmed/35746769 http://dx.doi.org/10.3390/v14061298 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Huang, Yi Zhang, Hongqing Li, Xiaodan Zhao, Lu Cai, Dirui Wang, Shunlong Ren, Nanjie Ma, Haixia Huang, Doudou Wang, Fei Yuan, Zhiming Zhang, Bo Xia, Han In Vitro and In Vivo Characterization of a New Strain of Mosquito Flavivirus Derived from Culicoides |
title | In Vitro and In Vivo Characterization of a New Strain of Mosquito Flavivirus Derived from Culicoides |
title_full | In Vitro and In Vivo Characterization of a New Strain of Mosquito Flavivirus Derived from Culicoides |
title_fullStr | In Vitro and In Vivo Characterization of a New Strain of Mosquito Flavivirus Derived from Culicoides |
title_full_unstemmed | In Vitro and In Vivo Characterization of a New Strain of Mosquito Flavivirus Derived from Culicoides |
title_short | In Vitro and In Vivo Characterization of a New Strain of Mosquito Flavivirus Derived from Culicoides |
title_sort | in vitro and in vivo characterization of a new strain of mosquito flavivirus derived from culicoides |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9229015/ https://www.ncbi.nlm.nih.gov/pubmed/35746769 http://dx.doi.org/10.3390/v14061298 |
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