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Escherichia/Shigella, SCFAs, and Metabolic Pathways—The Triad That Orchestrates Intestinal Dysbiosis in Patients with Decompensated Alcoholic Cirrhosis from Western Mexico

Gut microbiota undergoes profound alterations in alcohol cirrhosis. Microbiota-derived products, e.g., short chain fatty acids (SCFA), regulate the homeostasis of the gut-liver axis. The objective was to evaluate the composition and functions of the intestinal microbiota in patients with alcohol-dec...

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Autores principales: Baltazar-Díaz, Tonatiuh Abimael, González-Hernández, Luz Alicia, Aldana-Ledesma, Juan Manuel, Peña-Rodríguez, Marcela, Vega-Magaña, Alejandra Natali, Zepeda-Morales, Adelaida Sara Minia, López-Roa, Rocío Ivette, del Toro-Arreola, Susana, Martínez-López, Erika, Salazar-Montes, Adriana María, Bueno-Topete, Miriam Ruth
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9229093/
https://www.ncbi.nlm.nih.gov/pubmed/35744749
http://dx.doi.org/10.3390/microorganisms10061231
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author Baltazar-Díaz, Tonatiuh Abimael
González-Hernández, Luz Alicia
Aldana-Ledesma, Juan Manuel
Peña-Rodríguez, Marcela
Vega-Magaña, Alejandra Natali
Zepeda-Morales, Adelaida Sara Minia
López-Roa, Rocío Ivette
del Toro-Arreola, Susana
Martínez-López, Erika
Salazar-Montes, Adriana María
Bueno-Topete, Miriam Ruth
author_facet Baltazar-Díaz, Tonatiuh Abimael
González-Hernández, Luz Alicia
Aldana-Ledesma, Juan Manuel
Peña-Rodríguez, Marcela
Vega-Magaña, Alejandra Natali
Zepeda-Morales, Adelaida Sara Minia
López-Roa, Rocío Ivette
del Toro-Arreola, Susana
Martínez-López, Erika
Salazar-Montes, Adriana María
Bueno-Topete, Miriam Ruth
author_sort Baltazar-Díaz, Tonatiuh Abimael
collection PubMed
description Gut microbiota undergoes profound alterations in alcohol cirrhosis. Microbiota-derived products, e.g., short chain fatty acids (SCFA), regulate the homeostasis of the gut-liver axis. The objective was to evaluate the composition and functions of the intestinal microbiota in patients with alcohol-decompensated cirrhosis. Fecal samples of 18 patients and 18 healthy controls (HC) were obtained. Microbial composition was characterized by 16S rRNA amplicon sequencing, SCFA quantification was performed by gas chromatography (GC), and metagenomic predictive profiles were analyzed by PICRUSt2. Gut microbiota in the cirrhosis group revealed a significant increase in the pathogenic/pathobionts genera Escherichia/Shigella and Prevotella, a decrease in beneficial bacteria, such as Blautia, Faecalibacterium, and a decreased α-diversity (p < 0.001) compared to HC. Fecal SCFA concentrations were significantly reduced in the cirrhosis group (p < 0.001). PICRUSt2 analysis indicated a decrease in acetyl-CoA fermentation to butyrate, as well as an increase in pathways related to antibiotics resistance, and aromatic amino acid biosynthesis. These metabolic pathways have been poorly described in the progression of alcohol-related decompensated cirrhosis. The gut microbiota of these patients possesses a pathogenic/inflammatory environment; therefore, future strategies to balance intestinal dysbiosis should be implemented. These findings are described for the first time in the population of western Mexico.
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spelling pubmed-92290932022-06-25 Escherichia/Shigella, SCFAs, and Metabolic Pathways—The Triad That Orchestrates Intestinal Dysbiosis in Patients with Decompensated Alcoholic Cirrhosis from Western Mexico Baltazar-Díaz, Tonatiuh Abimael González-Hernández, Luz Alicia Aldana-Ledesma, Juan Manuel Peña-Rodríguez, Marcela Vega-Magaña, Alejandra Natali Zepeda-Morales, Adelaida Sara Minia López-Roa, Rocío Ivette del Toro-Arreola, Susana Martínez-López, Erika Salazar-Montes, Adriana María Bueno-Topete, Miriam Ruth Microorganisms Article Gut microbiota undergoes profound alterations in alcohol cirrhosis. Microbiota-derived products, e.g., short chain fatty acids (SCFA), regulate the homeostasis of the gut-liver axis. The objective was to evaluate the composition and functions of the intestinal microbiota in patients with alcohol-decompensated cirrhosis. Fecal samples of 18 patients and 18 healthy controls (HC) were obtained. Microbial composition was characterized by 16S rRNA amplicon sequencing, SCFA quantification was performed by gas chromatography (GC), and metagenomic predictive profiles were analyzed by PICRUSt2. Gut microbiota in the cirrhosis group revealed a significant increase in the pathogenic/pathobionts genera Escherichia/Shigella and Prevotella, a decrease in beneficial bacteria, such as Blautia, Faecalibacterium, and a decreased α-diversity (p < 0.001) compared to HC. Fecal SCFA concentrations were significantly reduced in the cirrhosis group (p < 0.001). PICRUSt2 analysis indicated a decrease in acetyl-CoA fermentation to butyrate, as well as an increase in pathways related to antibiotics resistance, and aromatic amino acid biosynthesis. These metabolic pathways have been poorly described in the progression of alcohol-related decompensated cirrhosis. The gut microbiota of these patients possesses a pathogenic/inflammatory environment; therefore, future strategies to balance intestinal dysbiosis should be implemented. These findings are described for the first time in the population of western Mexico. MDPI 2022-06-16 /pmc/articles/PMC9229093/ /pubmed/35744749 http://dx.doi.org/10.3390/microorganisms10061231 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Baltazar-Díaz, Tonatiuh Abimael
González-Hernández, Luz Alicia
Aldana-Ledesma, Juan Manuel
Peña-Rodríguez, Marcela
Vega-Magaña, Alejandra Natali
Zepeda-Morales, Adelaida Sara Minia
López-Roa, Rocío Ivette
del Toro-Arreola, Susana
Martínez-López, Erika
Salazar-Montes, Adriana María
Bueno-Topete, Miriam Ruth
Escherichia/Shigella, SCFAs, and Metabolic Pathways—The Triad That Orchestrates Intestinal Dysbiosis in Patients with Decompensated Alcoholic Cirrhosis from Western Mexico
title Escherichia/Shigella, SCFAs, and Metabolic Pathways—The Triad That Orchestrates Intestinal Dysbiosis in Patients with Decompensated Alcoholic Cirrhosis from Western Mexico
title_full Escherichia/Shigella, SCFAs, and Metabolic Pathways—The Triad That Orchestrates Intestinal Dysbiosis in Patients with Decompensated Alcoholic Cirrhosis from Western Mexico
title_fullStr Escherichia/Shigella, SCFAs, and Metabolic Pathways—The Triad That Orchestrates Intestinal Dysbiosis in Patients with Decompensated Alcoholic Cirrhosis from Western Mexico
title_full_unstemmed Escherichia/Shigella, SCFAs, and Metabolic Pathways—The Triad That Orchestrates Intestinal Dysbiosis in Patients with Decompensated Alcoholic Cirrhosis from Western Mexico
title_short Escherichia/Shigella, SCFAs, and Metabolic Pathways—The Triad That Orchestrates Intestinal Dysbiosis in Patients with Decompensated Alcoholic Cirrhosis from Western Mexico
title_sort escherichia/shigella, scfas, and metabolic pathways—the triad that orchestrates intestinal dysbiosis in patients with decompensated alcoholic cirrhosis from western mexico
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9229093/
https://www.ncbi.nlm.nih.gov/pubmed/35744749
http://dx.doi.org/10.3390/microorganisms10061231
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