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Gut archaea associated with bacteria colonization and succession during piglet weaning transitions

BACKGROUND: Host-associated gut microbial communities are key players in shaping the fitness and health of animals. However, most current studies have focused on the gut bacteria, neglecting important gut fungal and archaeal components of these communities. Here, we investigated the gut fungi and ar...

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Autores principales: Xiong, Xinwei, Rao, Yousheng, Tu, Xutang, Wang, Zhangfeng, Gong, Jishang, Yang, Yanbei, Wu, Haobin, Liu, Xianxian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9229118/
https://www.ncbi.nlm.nih.gov/pubmed/35751084
http://dx.doi.org/10.1186/s12917-022-03330-4
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author Xiong, Xinwei
Rao, Yousheng
Tu, Xutang
Wang, Zhangfeng
Gong, Jishang
Yang, Yanbei
Wu, Haobin
Liu, Xianxian
author_facet Xiong, Xinwei
Rao, Yousheng
Tu, Xutang
Wang, Zhangfeng
Gong, Jishang
Yang, Yanbei
Wu, Haobin
Liu, Xianxian
author_sort Xiong, Xinwei
collection PubMed
description BACKGROUND: Host-associated gut microbial communities are key players in shaping the fitness and health of animals. However, most current studies have focused on the gut bacteria, neglecting important gut fungal and archaeal components of these communities. Here, we investigated the gut fungi and archaea community composition in Large White piglets using shotgun metagenomic sequencing, and systematically evaluated how community composition association with gut microbiome, functional capacity, and serum metabolites varied across three weaning periods. RESULTS: We found that Mucoromycota, Ascomycota and Basidiomycota were the most common fungi phyla and Euryarchaeota was the most common archaea phyla across individuals. We identified that Methanosarcina siciliae was the most significantly different archaea species among three weaning periods, while Parasitella parasitica, the only differential fungi species, was significantly and positively correlated with Methanosarcina siciliae enriched in day 28 group. The random forest analysis also identified Methanosarcina siciliae and Parasitella parasitica as weaning-biased archaea and fungi at the species level. Additionally, Methanosarcina siciliae was significantly correlated with P. copri and the shifts of functional capacities of the gut microbiome and several CAZymes in day 28 group. Furthermore, characteristic successional alterations in gut archaea, fungi, bacteria, and serum metabolites with each weaning step revealed a weaning transition coexpression network, e.g., Methanosarcina siciliae and P. copri were positively and significantly correlated with 15-HEPE, 8-O-Methyloblongine, and Troxilin B3. CONCLUSION: Our findings provide a deep insight into the interactions among gut archaea, fungi, bacteria, and serum metabolites and will present a theoretical framework for understanding gut bacterial colonization and succession association with archaea during piglet weaning transitions. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12917-022-03330-4.
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spelling pubmed-92291182022-06-25 Gut archaea associated with bacteria colonization and succession during piglet weaning transitions Xiong, Xinwei Rao, Yousheng Tu, Xutang Wang, Zhangfeng Gong, Jishang Yang, Yanbei Wu, Haobin Liu, Xianxian BMC Vet Res Research BACKGROUND: Host-associated gut microbial communities are key players in shaping the fitness and health of animals. However, most current studies have focused on the gut bacteria, neglecting important gut fungal and archaeal components of these communities. Here, we investigated the gut fungi and archaea community composition in Large White piglets using shotgun metagenomic sequencing, and systematically evaluated how community composition association with gut microbiome, functional capacity, and serum metabolites varied across three weaning periods. RESULTS: We found that Mucoromycota, Ascomycota and Basidiomycota were the most common fungi phyla and Euryarchaeota was the most common archaea phyla across individuals. We identified that Methanosarcina siciliae was the most significantly different archaea species among three weaning periods, while Parasitella parasitica, the only differential fungi species, was significantly and positively correlated with Methanosarcina siciliae enriched in day 28 group. The random forest analysis also identified Methanosarcina siciliae and Parasitella parasitica as weaning-biased archaea and fungi at the species level. Additionally, Methanosarcina siciliae was significantly correlated with P. copri and the shifts of functional capacities of the gut microbiome and several CAZymes in day 28 group. Furthermore, characteristic successional alterations in gut archaea, fungi, bacteria, and serum metabolites with each weaning step revealed a weaning transition coexpression network, e.g., Methanosarcina siciliae and P. copri were positively and significantly correlated with 15-HEPE, 8-O-Methyloblongine, and Troxilin B3. CONCLUSION: Our findings provide a deep insight into the interactions among gut archaea, fungi, bacteria, and serum metabolites and will present a theoretical framework for understanding gut bacterial colonization and succession association with archaea during piglet weaning transitions. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12917-022-03330-4. BioMed Central 2022-06-24 /pmc/articles/PMC9229118/ /pubmed/35751084 http://dx.doi.org/10.1186/s12917-022-03330-4 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Xiong, Xinwei
Rao, Yousheng
Tu, Xutang
Wang, Zhangfeng
Gong, Jishang
Yang, Yanbei
Wu, Haobin
Liu, Xianxian
Gut archaea associated with bacteria colonization and succession during piglet weaning transitions
title Gut archaea associated with bacteria colonization and succession during piglet weaning transitions
title_full Gut archaea associated with bacteria colonization and succession during piglet weaning transitions
title_fullStr Gut archaea associated with bacteria colonization and succession during piglet weaning transitions
title_full_unstemmed Gut archaea associated with bacteria colonization and succession during piglet weaning transitions
title_short Gut archaea associated with bacteria colonization and succession during piglet weaning transitions
title_sort gut archaea associated with bacteria colonization and succession during piglet weaning transitions
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9229118/
https://www.ncbi.nlm.nih.gov/pubmed/35751084
http://dx.doi.org/10.1186/s12917-022-03330-4
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