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An Interlaboratory Comparison Study of Regulated and Emerging Mycotoxins Using Liquid Chromatography Mass Spectrometry: Challenges and Future Directions of Routine Multi-Mycotoxin Analysis including Emerging Mycotoxins
The present interlaboratory comparison study involved nine laboratories located throughout the world that tested for 24 regulated and non-regulated mycotoxins by applying their in-house LC-MS/MS multi-toxin method to 10 individual lots of 4 matrix commodities, including complex chicken and swine fee...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9229327/ https://www.ncbi.nlm.nih.gov/pubmed/35737066 http://dx.doi.org/10.3390/toxins14060405 |
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author | Steiner, David Humpel, Armin Stamminger, Eleonore Schoeberl, Anna Pachschwoell, Gerlinde Sloboda, Anita Swoboda, Christy Rigg, Jolene Zhang, Dawei Wang, Yahong Davis, Joshua Sulyok, Michael Krska, Rudolf Quinn, Brian Greer, Brett Elliott, Christopher T. Dzuman, Zbynek Hajslova, Jana Gschaider, Andreas Fechner, Carina Forstner, Lisa Varga, Elisabeth Jedziniak, Piotr Pietruszka, Katarzyna Rudawska, Adrianna Malachová, Alexandra |
author_facet | Steiner, David Humpel, Armin Stamminger, Eleonore Schoeberl, Anna Pachschwoell, Gerlinde Sloboda, Anita Swoboda, Christy Rigg, Jolene Zhang, Dawei Wang, Yahong Davis, Joshua Sulyok, Michael Krska, Rudolf Quinn, Brian Greer, Brett Elliott, Christopher T. Dzuman, Zbynek Hajslova, Jana Gschaider, Andreas Fechner, Carina Forstner, Lisa Varga, Elisabeth Jedziniak, Piotr Pietruszka, Katarzyna Rudawska, Adrianna Malachová, Alexandra |
author_sort | Steiner, David |
collection | PubMed |
description | The present interlaboratory comparison study involved nine laboratories located throughout the world that tested for 24 regulated and non-regulated mycotoxins by applying their in-house LC-MS/MS multi-toxin method to 10 individual lots of 4 matrix commodities, including complex chicken and swine feed, soy and corn gluten. In total, more than 6000 data points were collected and analyzed statistically by calculating a consensus value in combination with a target standard deviation following a modified Horwitz equation. The performance of each participant was evaluated by a z-score assessment with a satisfying range of ±2, leading to an overall success rate of 70% for all tested compounds. Equal performance for both regulated and emerging mycotoxins indicates that participating routine laboratories have successfully expanded their analytical portfolio in view of potentially new regulations. In addition, the study design proved to be fit for the purpose of providing future certified reference materials, which surpass current analyte matrix combinations and exceed the typical scope of the regulatory framework. |
format | Online Article Text |
id | pubmed-9229327 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-92293272022-06-25 An Interlaboratory Comparison Study of Regulated and Emerging Mycotoxins Using Liquid Chromatography Mass Spectrometry: Challenges and Future Directions of Routine Multi-Mycotoxin Analysis including Emerging Mycotoxins Steiner, David Humpel, Armin Stamminger, Eleonore Schoeberl, Anna Pachschwoell, Gerlinde Sloboda, Anita Swoboda, Christy Rigg, Jolene Zhang, Dawei Wang, Yahong Davis, Joshua Sulyok, Michael Krska, Rudolf Quinn, Brian Greer, Brett Elliott, Christopher T. Dzuman, Zbynek Hajslova, Jana Gschaider, Andreas Fechner, Carina Forstner, Lisa Varga, Elisabeth Jedziniak, Piotr Pietruszka, Katarzyna Rudawska, Adrianna Malachová, Alexandra Toxins (Basel) Article The present interlaboratory comparison study involved nine laboratories located throughout the world that tested for 24 regulated and non-regulated mycotoxins by applying their in-house LC-MS/MS multi-toxin method to 10 individual lots of 4 matrix commodities, including complex chicken and swine feed, soy and corn gluten. In total, more than 6000 data points were collected and analyzed statistically by calculating a consensus value in combination with a target standard deviation following a modified Horwitz equation. The performance of each participant was evaluated by a z-score assessment with a satisfying range of ±2, leading to an overall success rate of 70% for all tested compounds. Equal performance for both regulated and emerging mycotoxins indicates that participating routine laboratories have successfully expanded their analytical portfolio in view of potentially new regulations. In addition, the study design proved to be fit for the purpose of providing future certified reference materials, which surpass current analyte matrix combinations and exceed the typical scope of the regulatory framework. MDPI 2022-06-13 /pmc/articles/PMC9229327/ /pubmed/35737066 http://dx.doi.org/10.3390/toxins14060405 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Steiner, David Humpel, Armin Stamminger, Eleonore Schoeberl, Anna Pachschwoell, Gerlinde Sloboda, Anita Swoboda, Christy Rigg, Jolene Zhang, Dawei Wang, Yahong Davis, Joshua Sulyok, Michael Krska, Rudolf Quinn, Brian Greer, Brett Elliott, Christopher T. Dzuman, Zbynek Hajslova, Jana Gschaider, Andreas Fechner, Carina Forstner, Lisa Varga, Elisabeth Jedziniak, Piotr Pietruszka, Katarzyna Rudawska, Adrianna Malachová, Alexandra An Interlaboratory Comparison Study of Regulated and Emerging Mycotoxins Using Liquid Chromatography Mass Spectrometry: Challenges and Future Directions of Routine Multi-Mycotoxin Analysis including Emerging Mycotoxins |
title | An Interlaboratory Comparison Study of Regulated and Emerging Mycotoxins Using Liquid Chromatography Mass Spectrometry: Challenges and Future Directions of Routine Multi-Mycotoxin Analysis including Emerging Mycotoxins |
title_full | An Interlaboratory Comparison Study of Regulated and Emerging Mycotoxins Using Liquid Chromatography Mass Spectrometry: Challenges and Future Directions of Routine Multi-Mycotoxin Analysis including Emerging Mycotoxins |
title_fullStr | An Interlaboratory Comparison Study of Regulated and Emerging Mycotoxins Using Liquid Chromatography Mass Spectrometry: Challenges and Future Directions of Routine Multi-Mycotoxin Analysis including Emerging Mycotoxins |
title_full_unstemmed | An Interlaboratory Comparison Study of Regulated and Emerging Mycotoxins Using Liquid Chromatography Mass Spectrometry: Challenges and Future Directions of Routine Multi-Mycotoxin Analysis including Emerging Mycotoxins |
title_short | An Interlaboratory Comparison Study of Regulated and Emerging Mycotoxins Using Liquid Chromatography Mass Spectrometry: Challenges and Future Directions of Routine Multi-Mycotoxin Analysis including Emerging Mycotoxins |
title_sort | interlaboratory comparison study of regulated and emerging mycotoxins using liquid chromatography mass spectrometry: challenges and future directions of routine multi-mycotoxin analysis including emerging mycotoxins |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9229327/ https://www.ncbi.nlm.nih.gov/pubmed/35737066 http://dx.doi.org/10.3390/toxins14060405 |
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