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Development of an Antibacterial Dentin Adhesive
Nisin is a peptide that possesses potent antibacterial properties. This study evaluated the antibacterial activity of a nisin-doped adhesive against Streptococcus mutans, as well as its degree of conversion and microtensile bond strength (μTBS) to dentin. Nisin was added to the adhesive Adper Single...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9229334/ https://www.ncbi.nlm.nih.gov/pubmed/35746077 http://dx.doi.org/10.3390/polym14122502 |
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author | Lopes, Stephanie R. Matuda, Amanda G. N. Campos, Raquel P. Mafetano, Ana Paula V. P. Barnabe, Ana Helena M. Chagas, Gabriela S. Barcellos, Daphne C. Niu, Li-Na Tay, Franklin R. Pucci, Cesar R. |
author_facet | Lopes, Stephanie R. Matuda, Amanda G. N. Campos, Raquel P. Mafetano, Ana Paula V. P. Barnabe, Ana Helena M. Chagas, Gabriela S. Barcellos, Daphne C. Niu, Li-Na Tay, Franklin R. Pucci, Cesar R. |
author_sort | Lopes, Stephanie R. |
collection | PubMed |
description | Nisin is a peptide that possesses potent antibacterial properties. This study evaluated the antibacterial activity of a nisin-doped adhesive against Streptococcus mutans, as well as its degree of conversion and microtensile bond strength (μTBS) to dentin. Nisin was added to the adhesive Adper Single Bond 2 (3M ESPE), resulting in four groups: Control Group (Single Bond 2); Group 1% (1 wt% nisin-incorporated), Group 3% (3 wt% nisin-incorporated) and Group 5% (5 wt% nisin-incorporated). Antibacterial activity against S. mutans was evaluated using colony-forming unit counts (CFU). The degree of conversion was tested using FTIR. Forty human teeth were restored for μTBS evaluation. Data were statistically analyzed with ANOVA and Tukey tests at α = 0.05. The nisin-doped adhesives, for all concentrations, exhibited a significant inhibition of the growth of S. mutans (p < 0.05); Incorporation of 5% and 3% nisin decreased the degree of conversion of the adhesive (p < 0.05). The μTBS (in MPa): Control Group—38.3 ± 2.3(A), Group 1%—35.6 ± 2.1(A), Group 3%—27.1 ± 1.6(B) and Group 5%—22.3 ± 1.0(C). Nisin-doped adhesives exerted a bactericidal effect on S. mutans. The μTBS and degree of conversion of adhesive were not affected after incorporation of 1% nisin. |
format | Online Article Text |
id | pubmed-9229334 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-92293342022-06-25 Development of an Antibacterial Dentin Adhesive Lopes, Stephanie R. Matuda, Amanda G. N. Campos, Raquel P. Mafetano, Ana Paula V. P. Barnabe, Ana Helena M. Chagas, Gabriela S. Barcellos, Daphne C. Niu, Li-Na Tay, Franklin R. Pucci, Cesar R. Polymers (Basel) Article Nisin is a peptide that possesses potent antibacterial properties. This study evaluated the antibacterial activity of a nisin-doped adhesive against Streptococcus mutans, as well as its degree of conversion and microtensile bond strength (μTBS) to dentin. Nisin was added to the adhesive Adper Single Bond 2 (3M ESPE), resulting in four groups: Control Group (Single Bond 2); Group 1% (1 wt% nisin-incorporated), Group 3% (3 wt% nisin-incorporated) and Group 5% (5 wt% nisin-incorporated). Antibacterial activity against S. mutans was evaluated using colony-forming unit counts (CFU). The degree of conversion was tested using FTIR. Forty human teeth were restored for μTBS evaluation. Data were statistically analyzed with ANOVA and Tukey tests at α = 0.05. The nisin-doped adhesives, for all concentrations, exhibited a significant inhibition of the growth of S. mutans (p < 0.05); Incorporation of 5% and 3% nisin decreased the degree of conversion of the adhesive (p < 0.05). The μTBS (in MPa): Control Group—38.3 ± 2.3(A), Group 1%—35.6 ± 2.1(A), Group 3%—27.1 ± 1.6(B) and Group 5%—22.3 ± 1.0(C). Nisin-doped adhesives exerted a bactericidal effect on S. mutans. The μTBS and degree of conversion of adhesive were not affected after incorporation of 1% nisin. MDPI 2022-06-19 /pmc/articles/PMC9229334/ /pubmed/35746077 http://dx.doi.org/10.3390/polym14122502 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Lopes, Stephanie R. Matuda, Amanda G. N. Campos, Raquel P. Mafetano, Ana Paula V. P. Barnabe, Ana Helena M. Chagas, Gabriela S. Barcellos, Daphne C. Niu, Li-Na Tay, Franklin R. Pucci, Cesar R. Development of an Antibacterial Dentin Adhesive |
title | Development of an Antibacterial Dentin Adhesive |
title_full | Development of an Antibacterial Dentin Adhesive |
title_fullStr | Development of an Antibacterial Dentin Adhesive |
title_full_unstemmed | Development of an Antibacterial Dentin Adhesive |
title_short | Development of an Antibacterial Dentin Adhesive |
title_sort | development of an antibacterial dentin adhesive |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9229334/ https://www.ncbi.nlm.nih.gov/pubmed/35746077 http://dx.doi.org/10.3390/polym14122502 |
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