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Enhancing Red Yeast Biomass Yield and Lipid Biosynthesis by Using Waste Nitrogen Source by Glucose Fed-Batch at Low Temperature
This work reports the effect of simple feeding strategies and temperature to obtain high-cell-density cultures of Rhodotorula glutinis var. rubescens LOCKR13 maximizing the de novo lipid productivity using deproteinated potato wastewater (DPW) as a basic medium. Feeding DPW with glucose enables a hi...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9229382/ https://www.ncbi.nlm.nih.gov/pubmed/35744771 http://dx.doi.org/10.3390/microorganisms10061253 |
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author | Gientka, Iwona Wirkowska-Wojdyła, Magdalena Ostrowska-Ligęza, Ewa Janowicz, Monika Reczek, Lidia Synowiec, Alicja Błażejak, Stanisław |
author_facet | Gientka, Iwona Wirkowska-Wojdyła, Magdalena Ostrowska-Ligęza, Ewa Janowicz, Monika Reczek, Lidia Synowiec, Alicja Błażejak, Stanisław |
author_sort | Gientka, Iwona |
collection | PubMed |
description | This work reports the effect of simple feeding strategies and temperature to obtain high-cell-density cultures of Rhodotorula glutinis var. rubescens LOCKR13 maximizing the de novo lipid productivity using deproteinated potato wastewater (DPW) as a basic medium. Feeding DPW with glucose enables a high yield of Rhodotorula glutinis var. rubescens LOCKR13 biomass (52 g (d.w.) L(−1)) to be obtained. The highest values of lipid accumulation (34.15%, w/w), production (14.68 g L(−1)) and yield coefficients (Y(L/S): 0.242 g g(−1)), and volumetric productivity (P(L): 0.1 g L(−1) h(−1)) were reached by the strain in the two-stage fed-batch process at 20 °C. The lipid of yeast biomass was rich in oleic acid ((Δ9)C18:1) and palmitic acid (C16:0), and the lower temperature of incubation significantly increased the MUFA (especially oleic acid) content. For the first time, a unique set of thermal analyses of the microbial oil was performed. The isotherms of the oxidation kinetics (PDSC) showed that lipids extracted from the biomass of red yeast had high oxidative stability. This feature of the yeast oil can be useful for long-shelf-life food products and can be promising for the production of biodiesel. |
format | Online Article Text |
id | pubmed-9229382 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-92293822022-06-25 Enhancing Red Yeast Biomass Yield and Lipid Biosynthesis by Using Waste Nitrogen Source by Glucose Fed-Batch at Low Temperature Gientka, Iwona Wirkowska-Wojdyła, Magdalena Ostrowska-Ligęza, Ewa Janowicz, Monika Reczek, Lidia Synowiec, Alicja Błażejak, Stanisław Microorganisms Article This work reports the effect of simple feeding strategies and temperature to obtain high-cell-density cultures of Rhodotorula glutinis var. rubescens LOCKR13 maximizing the de novo lipid productivity using deproteinated potato wastewater (DPW) as a basic medium. Feeding DPW with glucose enables a high yield of Rhodotorula glutinis var. rubescens LOCKR13 biomass (52 g (d.w.) L(−1)) to be obtained. The highest values of lipid accumulation (34.15%, w/w), production (14.68 g L(−1)) and yield coefficients (Y(L/S): 0.242 g g(−1)), and volumetric productivity (P(L): 0.1 g L(−1) h(−1)) were reached by the strain in the two-stage fed-batch process at 20 °C. The lipid of yeast biomass was rich in oleic acid ((Δ9)C18:1) and palmitic acid (C16:0), and the lower temperature of incubation significantly increased the MUFA (especially oleic acid) content. For the first time, a unique set of thermal analyses of the microbial oil was performed. The isotherms of the oxidation kinetics (PDSC) showed that lipids extracted from the biomass of red yeast had high oxidative stability. This feature of the yeast oil can be useful for long-shelf-life food products and can be promising for the production of biodiesel. MDPI 2022-06-20 /pmc/articles/PMC9229382/ /pubmed/35744771 http://dx.doi.org/10.3390/microorganisms10061253 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Gientka, Iwona Wirkowska-Wojdyła, Magdalena Ostrowska-Ligęza, Ewa Janowicz, Monika Reczek, Lidia Synowiec, Alicja Błażejak, Stanisław Enhancing Red Yeast Biomass Yield and Lipid Biosynthesis by Using Waste Nitrogen Source by Glucose Fed-Batch at Low Temperature |
title | Enhancing Red Yeast Biomass Yield and Lipid Biosynthesis by Using Waste Nitrogen Source by Glucose Fed-Batch at Low Temperature |
title_full | Enhancing Red Yeast Biomass Yield and Lipid Biosynthesis by Using Waste Nitrogen Source by Glucose Fed-Batch at Low Temperature |
title_fullStr | Enhancing Red Yeast Biomass Yield and Lipid Biosynthesis by Using Waste Nitrogen Source by Glucose Fed-Batch at Low Temperature |
title_full_unstemmed | Enhancing Red Yeast Biomass Yield and Lipid Biosynthesis by Using Waste Nitrogen Source by Glucose Fed-Batch at Low Temperature |
title_short | Enhancing Red Yeast Biomass Yield and Lipid Biosynthesis by Using Waste Nitrogen Source by Glucose Fed-Batch at Low Temperature |
title_sort | enhancing red yeast biomass yield and lipid biosynthesis by using waste nitrogen source by glucose fed-batch at low temperature |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9229382/ https://www.ncbi.nlm.nih.gov/pubmed/35744771 http://dx.doi.org/10.3390/microorganisms10061253 |
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