Cargando…

Surface Hydrophilic Modification for Chip of Centrifugal Microfluidic Immunoassay System

The surface of a centrifugal microfluidic immunoassay system chip such as polymethyl methacrylate (PMMA) is often hydrophobic, which leads to problems such as poor liquid transfer efficiency and easy-to-block siphon channels, leading to bad fluid control. Therefore, surface hydrophilic modification...

Descripción completa

Detalles Bibliográficos
Autores principales: Shi, Yuxing, Ye, Peng, Wang, Chuang, Yang, Kuojun, Guo, Jinhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9229454/
https://www.ncbi.nlm.nih.gov/pubmed/35744444
http://dx.doi.org/10.3390/mi13060831
_version_ 1784734751414812672
author Shi, Yuxing
Ye, Peng
Wang, Chuang
Yang, Kuojun
Guo, Jinhong
author_facet Shi, Yuxing
Ye, Peng
Wang, Chuang
Yang, Kuojun
Guo, Jinhong
author_sort Shi, Yuxing
collection PubMed
description The surface of a centrifugal microfluidic immunoassay system chip such as polymethyl methacrylate (PMMA) is often hydrophobic, which leads to problems such as poor liquid transfer efficiency and easy-to-block siphon channels, leading to bad fluid control. Therefore, surface hydrophilic modification for such chips is necessary to improve the rapidity and sensitivity of the system. Chemical modification is commonly used, but there is little research on the hydrophilic effect of different concentrations of hydrophilic reagents. According to function requirements for different microchannels of the chip (some only need to ensure the liquid can flow into the next chamber, and some also need to ensure the function of “closing the door” during immunoassay incubation), we explored the best combination of hydrophilic reagent and concentration through experiments. Firstly, three hydrophilic reagents were used for modification. Secondly, the hydrophilic effects of different reagents and concentrations were explored by contact angle test, the influence of different modification methods on liquid transfer efficiency was characterized by residual liquid calculation in the chamber. Finally, the effect of different hydrophilic reagents on absorbance was also tested. By experimental results and comprehensively considering the stability of the modification effect and the function requirements, Tween-20 (2.0% v/v) was chosen as the modifying reagents of the first siphon valve and the second siphon valve, and TritonX-100 (2.0% v/v) was chosen for the third siphon valve, which effectively reduces the contact angle and improves the liquid transfer efficiency, leading to further improvement of the rapidity and sensitivity of the centrifugal microfluidic immunoassay system by efficient siphoning and high plasma separation efficiency (99%).
format Online
Article
Text
id pubmed-9229454
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-92294542022-06-25 Surface Hydrophilic Modification for Chip of Centrifugal Microfluidic Immunoassay System Shi, Yuxing Ye, Peng Wang, Chuang Yang, Kuojun Guo, Jinhong Micromachines (Basel) Article The surface of a centrifugal microfluidic immunoassay system chip such as polymethyl methacrylate (PMMA) is often hydrophobic, which leads to problems such as poor liquid transfer efficiency and easy-to-block siphon channels, leading to bad fluid control. Therefore, surface hydrophilic modification for such chips is necessary to improve the rapidity and sensitivity of the system. Chemical modification is commonly used, but there is little research on the hydrophilic effect of different concentrations of hydrophilic reagents. According to function requirements for different microchannels of the chip (some only need to ensure the liquid can flow into the next chamber, and some also need to ensure the function of “closing the door” during immunoassay incubation), we explored the best combination of hydrophilic reagent and concentration through experiments. Firstly, three hydrophilic reagents were used for modification. Secondly, the hydrophilic effects of different reagents and concentrations were explored by contact angle test, the influence of different modification methods on liquid transfer efficiency was characterized by residual liquid calculation in the chamber. Finally, the effect of different hydrophilic reagents on absorbance was also tested. By experimental results and comprehensively considering the stability of the modification effect and the function requirements, Tween-20 (2.0% v/v) was chosen as the modifying reagents of the first siphon valve and the second siphon valve, and TritonX-100 (2.0% v/v) was chosen for the third siphon valve, which effectively reduces the contact angle and improves the liquid transfer efficiency, leading to further improvement of the rapidity and sensitivity of the centrifugal microfluidic immunoassay system by efficient siphoning and high plasma separation efficiency (99%). MDPI 2022-05-26 /pmc/articles/PMC9229454/ /pubmed/35744444 http://dx.doi.org/10.3390/mi13060831 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Shi, Yuxing
Ye, Peng
Wang, Chuang
Yang, Kuojun
Guo, Jinhong
Surface Hydrophilic Modification for Chip of Centrifugal Microfluidic Immunoassay System
title Surface Hydrophilic Modification for Chip of Centrifugal Microfluidic Immunoassay System
title_full Surface Hydrophilic Modification for Chip of Centrifugal Microfluidic Immunoassay System
title_fullStr Surface Hydrophilic Modification for Chip of Centrifugal Microfluidic Immunoassay System
title_full_unstemmed Surface Hydrophilic Modification for Chip of Centrifugal Microfluidic Immunoassay System
title_short Surface Hydrophilic Modification for Chip of Centrifugal Microfluidic Immunoassay System
title_sort surface hydrophilic modification for chip of centrifugal microfluidic immunoassay system
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9229454/
https://www.ncbi.nlm.nih.gov/pubmed/35744444
http://dx.doi.org/10.3390/mi13060831
work_keys_str_mv AT shiyuxing surfacehydrophilicmodificationforchipofcentrifugalmicrofluidicimmunoassaysystem
AT yepeng surfacehydrophilicmodificationforchipofcentrifugalmicrofluidicimmunoassaysystem
AT wangchuang surfacehydrophilicmodificationforchipofcentrifugalmicrofluidicimmunoassaysystem
AT yangkuojun surfacehydrophilicmodificationforchipofcentrifugalmicrofluidicimmunoassaysystem
AT guojinhong surfacehydrophilicmodificationforchipofcentrifugalmicrofluidicimmunoassaysystem