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Simultaneous Removal of Mycotoxins by a New Feed Additive Containing a Tri-Octahedral Smectite Mixed with Lignocellulose

Simultaneous removal of mycotoxins has been poorly addressed, and a limited number of studies have reported the efficacy of feed additives in sequestering a large spectrum of mycotoxins. In this study, a new mycotoxin-adsorbing agent was obtained by properly mixing a tri-octahedral smectite with a l...

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Autores principales: Greco, Donato, D’Ascanio, Vito, Abbasciano, Mariagrazia, Santovito, Elisa, Garbetta, Antonella, Logrieco, Antonio F., Avantaggiato, Giuseppina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9229468/
https://www.ncbi.nlm.nih.gov/pubmed/35737054
http://dx.doi.org/10.3390/toxins14060393
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author Greco, Donato
D’Ascanio, Vito
Abbasciano, Mariagrazia
Santovito, Elisa
Garbetta, Antonella
Logrieco, Antonio F.
Avantaggiato, Giuseppina
author_facet Greco, Donato
D’Ascanio, Vito
Abbasciano, Mariagrazia
Santovito, Elisa
Garbetta, Antonella
Logrieco, Antonio F.
Avantaggiato, Giuseppina
author_sort Greco, Donato
collection PubMed
description Simultaneous removal of mycotoxins has been poorly addressed, and a limited number of studies have reported the efficacy of feed additives in sequestering a large spectrum of mycotoxins. In this study, a new mycotoxin-adsorbing agent was obtained by properly mixing a tri-octahedral smectite with a lignocellulose-based material. At a dosage of 1 mg mL(−1), these materials simultaneously adsorbed frequently occurring mycotoxins and did not exert a cytotoxic effect on intestinal cells. Chyme samples obtained by a simulated GI digestion did not affect the viability of Caco-2TC7 cells as measured by the MTT test. In addition, the chyme of the lignocellulose showed a high content of polyphenols (210 mg mL(−1) catechin equivalent) and good antioxidant activity. The properties of the individual constituents were maintained in the final composite, and were unaffected by their combination. When tested with a pool of seven mycotoxins at 1 µg mL(−1) each and pH 5, the composite (5 mg mL(−1)) simultaneously sequestered AFB(1) (95%), FB(1) (99%), ZEA (93%), OTA (80%), T-2 (63%), and DON (22%). HT-2 adsorption did not occur. Mycotoxin adsorption increased exponentially as dosage increased, and occurred at physiological pH values. AFB(1), ZEA and T-2 adsorption was not affected by pH in the range 3–9, whereas OTA and FB(1) were adsorbed at pH values of 3–5. The adsorbed amount of AFB(1), ZEA and T-2 was not released when pH rose from 3 to 7. FB(1) and OTA desorption was less than 38%. Langmuir adsorption isotherms revealed high capacity and affinity for adsorption of the target mycotoxins. Results of this study are promising and show the potential of the new composite to remove mycotoxins in practical scenarios where several mycotoxins can co-occur.
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spelling pubmed-92294682022-06-25 Simultaneous Removal of Mycotoxins by a New Feed Additive Containing a Tri-Octahedral Smectite Mixed with Lignocellulose Greco, Donato D’Ascanio, Vito Abbasciano, Mariagrazia Santovito, Elisa Garbetta, Antonella Logrieco, Antonio F. Avantaggiato, Giuseppina Toxins (Basel) Article Simultaneous removal of mycotoxins has been poorly addressed, and a limited number of studies have reported the efficacy of feed additives in sequestering a large spectrum of mycotoxins. In this study, a new mycotoxin-adsorbing agent was obtained by properly mixing a tri-octahedral smectite with a lignocellulose-based material. At a dosage of 1 mg mL(−1), these materials simultaneously adsorbed frequently occurring mycotoxins and did not exert a cytotoxic effect on intestinal cells. Chyme samples obtained by a simulated GI digestion did not affect the viability of Caco-2TC7 cells as measured by the MTT test. In addition, the chyme of the lignocellulose showed a high content of polyphenols (210 mg mL(−1) catechin equivalent) and good antioxidant activity. The properties of the individual constituents were maintained in the final composite, and were unaffected by their combination. When tested with a pool of seven mycotoxins at 1 µg mL(−1) each and pH 5, the composite (5 mg mL(−1)) simultaneously sequestered AFB(1) (95%), FB(1) (99%), ZEA (93%), OTA (80%), T-2 (63%), and DON (22%). HT-2 adsorption did not occur. Mycotoxin adsorption increased exponentially as dosage increased, and occurred at physiological pH values. AFB(1), ZEA and T-2 adsorption was not affected by pH in the range 3–9, whereas OTA and FB(1) were adsorbed at pH values of 3–5. The adsorbed amount of AFB(1), ZEA and T-2 was not released when pH rose from 3 to 7. FB(1) and OTA desorption was less than 38%. Langmuir adsorption isotherms revealed high capacity and affinity for adsorption of the target mycotoxins. Results of this study are promising and show the potential of the new composite to remove mycotoxins in practical scenarios where several mycotoxins can co-occur. MDPI 2022-06-08 /pmc/articles/PMC9229468/ /pubmed/35737054 http://dx.doi.org/10.3390/toxins14060393 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Greco, Donato
D’Ascanio, Vito
Abbasciano, Mariagrazia
Santovito, Elisa
Garbetta, Antonella
Logrieco, Antonio F.
Avantaggiato, Giuseppina
Simultaneous Removal of Mycotoxins by a New Feed Additive Containing a Tri-Octahedral Smectite Mixed with Lignocellulose
title Simultaneous Removal of Mycotoxins by a New Feed Additive Containing a Tri-Octahedral Smectite Mixed with Lignocellulose
title_full Simultaneous Removal of Mycotoxins by a New Feed Additive Containing a Tri-Octahedral Smectite Mixed with Lignocellulose
title_fullStr Simultaneous Removal of Mycotoxins by a New Feed Additive Containing a Tri-Octahedral Smectite Mixed with Lignocellulose
title_full_unstemmed Simultaneous Removal of Mycotoxins by a New Feed Additive Containing a Tri-Octahedral Smectite Mixed with Lignocellulose
title_short Simultaneous Removal of Mycotoxins by a New Feed Additive Containing a Tri-Octahedral Smectite Mixed with Lignocellulose
title_sort simultaneous removal of mycotoxins by a new feed additive containing a tri-octahedral smectite mixed with lignocellulose
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9229468/
https://www.ncbi.nlm.nih.gov/pubmed/35737054
http://dx.doi.org/10.3390/toxins14060393
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