Cargando…
Effects of Inulin-Based Prebiotics Alone or in Combination with Probiotics on Human Gut Microbiota and Markers of Immune System: A Randomized, Double-Blind, Placebo-Controlled Study in Healthy Subjects
The gut microbiota is implicated in diverse interactions affecting human health. The present study reports a randomized, double-blind, placebo-controlled clinical study conducted by administering a new synbiotic formulation composed of two Lactobacillus strains (L. plantarum and L. acidophilus) and...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9229734/ https://www.ncbi.nlm.nih.gov/pubmed/35744774 http://dx.doi.org/10.3390/microorganisms10061256 |
_version_ | 1784734825427501056 |
---|---|
author | De Giani, Alessandra Sandionigi, Anna Zampolli, Jessica Michelotti, Angela Tursi, Francesco Labra, Massimo Di Gennaro, Patrizia |
author_facet | De Giani, Alessandra Sandionigi, Anna Zampolli, Jessica Michelotti, Angela Tursi, Francesco Labra, Massimo Di Gennaro, Patrizia |
author_sort | De Giani, Alessandra |
collection | PubMed |
description | The gut microbiota is implicated in diverse interactions affecting human health. The present study reports a randomized, double-blind, placebo-controlled clinical study conducted by administering a new synbiotic formulation composed of two Lactobacillus strains (L. plantarum and L. acidophilus) and one Bifidobacterium strain (B. animalis subsp. lactis) and two types of fructans (fructo-oligosaccharides with a degree of polymerization of 3–5 and inulin-type fructans with 10 DP). The effects of this synbiotic were evaluated on healthy subjects for 28 days and the maintenance of its efficacy was evaluated at the end of a follow-up period of 28 days. The synbiotic treatment contributes to higher biodiversity of the gut microbiota, increasing the community richness with respect to the group with the prebiotics alone and the placebo group. Its positive effect is also reflected in the variation of microbial community structure favoring the beneficial short-chain fatty acids bacterial producers. The amelioration of the health status of the subjects was also established by the reduction of common infectious disease symptom incidence, the stimulation of the gut immune system showing a noteworthy variation of fecal β-defensin2 and calprotectin levels, and the modulation of the response of the respiratory tract’s immune system by salivary IgA as well as total antioxidant capacity biomarkers. |
format | Online Article Text |
id | pubmed-9229734 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-92297342022-06-25 Effects of Inulin-Based Prebiotics Alone or in Combination with Probiotics on Human Gut Microbiota and Markers of Immune System: A Randomized, Double-Blind, Placebo-Controlled Study in Healthy Subjects De Giani, Alessandra Sandionigi, Anna Zampolli, Jessica Michelotti, Angela Tursi, Francesco Labra, Massimo Di Gennaro, Patrizia Microorganisms Article The gut microbiota is implicated in diverse interactions affecting human health. The present study reports a randomized, double-blind, placebo-controlled clinical study conducted by administering a new synbiotic formulation composed of two Lactobacillus strains (L. plantarum and L. acidophilus) and one Bifidobacterium strain (B. animalis subsp. lactis) and two types of fructans (fructo-oligosaccharides with a degree of polymerization of 3–5 and inulin-type fructans with 10 DP). The effects of this synbiotic were evaluated on healthy subjects for 28 days and the maintenance of its efficacy was evaluated at the end of a follow-up period of 28 days. The synbiotic treatment contributes to higher biodiversity of the gut microbiota, increasing the community richness with respect to the group with the prebiotics alone and the placebo group. Its positive effect is also reflected in the variation of microbial community structure favoring the beneficial short-chain fatty acids bacterial producers. The amelioration of the health status of the subjects was also established by the reduction of common infectious disease symptom incidence, the stimulation of the gut immune system showing a noteworthy variation of fecal β-defensin2 and calprotectin levels, and the modulation of the response of the respiratory tract’s immune system by salivary IgA as well as total antioxidant capacity biomarkers. MDPI 2022-06-20 /pmc/articles/PMC9229734/ /pubmed/35744774 http://dx.doi.org/10.3390/microorganisms10061256 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article De Giani, Alessandra Sandionigi, Anna Zampolli, Jessica Michelotti, Angela Tursi, Francesco Labra, Massimo Di Gennaro, Patrizia Effects of Inulin-Based Prebiotics Alone or in Combination with Probiotics on Human Gut Microbiota and Markers of Immune System: A Randomized, Double-Blind, Placebo-Controlled Study in Healthy Subjects |
title | Effects of Inulin-Based Prebiotics Alone or in Combination with Probiotics on Human Gut Microbiota and Markers of Immune System: A Randomized, Double-Blind, Placebo-Controlled Study in Healthy Subjects |
title_full | Effects of Inulin-Based Prebiotics Alone or in Combination with Probiotics on Human Gut Microbiota and Markers of Immune System: A Randomized, Double-Blind, Placebo-Controlled Study in Healthy Subjects |
title_fullStr | Effects of Inulin-Based Prebiotics Alone or in Combination with Probiotics on Human Gut Microbiota and Markers of Immune System: A Randomized, Double-Blind, Placebo-Controlled Study in Healthy Subjects |
title_full_unstemmed | Effects of Inulin-Based Prebiotics Alone or in Combination with Probiotics on Human Gut Microbiota and Markers of Immune System: A Randomized, Double-Blind, Placebo-Controlled Study in Healthy Subjects |
title_short | Effects of Inulin-Based Prebiotics Alone or in Combination with Probiotics on Human Gut Microbiota and Markers of Immune System: A Randomized, Double-Blind, Placebo-Controlled Study in Healthy Subjects |
title_sort | effects of inulin-based prebiotics alone or in combination with probiotics on human gut microbiota and markers of immune system: a randomized, double-blind, placebo-controlled study in healthy subjects |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9229734/ https://www.ncbi.nlm.nih.gov/pubmed/35744774 http://dx.doi.org/10.3390/microorganisms10061256 |
work_keys_str_mv | AT degianialessandra effectsofinulinbasedprebioticsaloneorincombinationwithprobioticsonhumangutmicrobiotaandmarkersofimmunesystemarandomizeddoubleblindplacebocontrolledstudyinhealthysubjects AT sandionigianna effectsofinulinbasedprebioticsaloneorincombinationwithprobioticsonhumangutmicrobiotaandmarkersofimmunesystemarandomizeddoubleblindplacebocontrolledstudyinhealthysubjects AT zampollijessica effectsofinulinbasedprebioticsaloneorincombinationwithprobioticsonhumangutmicrobiotaandmarkersofimmunesystemarandomizeddoubleblindplacebocontrolledstudyinhealthysubjects AT michelottiangela effectsofinulinbasedprebioticsaloneorincombinationwithprobioticsonhumangutmicrobiotaandmarkersofimmunesystemarandomizeddoubleblindplacebocontrolledstudyinhealthysubjects AT tursifrancesco effectsofinulinbasedprebioticsaloneorincombinationwithprobioticsonhumangutmicrobiotaandmarkersofimmunesystemarandomizeddoubleblindplacebocontrolledstudyinhealthysubjects AT labramassimo effectsofinulinbasedprebioticsaloneorincombinationwithprobioticsonhumangutmicrobiotaandmarkersofimmunesystemarandomizeddoubleblindplacebocontrolledstudyinhealthysubjects AT digennaropatrizia effectsofinulinbasedprebioticsaloneorincombinationwithprobioticsonhumangutmicrobiotaandmarkersofimmunesystemarandomizeddoubleblindplacebocontrolledstudyinhealthysubjects |