Optimization of Cas9 RNA sequence to reduce its unexpected effects as a microRNA sponge

Cas9 RNA functions as a miRNA sponge. Let-7 is the dominant regulated miRNA by Cas9 RNA. RNA sequence optimization of Cas9 by synonymous mutation improves its safety. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12943-022-01604-x.

Detalles Bibliográficos
Autores principales: Jiang, Junfeng, Zeng, Tao, Zhang, Li, Fan, Xingfei, Jin, Qishu, Ni, Haitao, Ye, Yusheng, Cheng, Lipeng, Li, Li, Wang, Liujun, Xu, Sha, Yang, Yu, Gu, Juan, Guo, Bing, Wang, Lei, Li, Xin, Qin, Yingyi, Li, Jiaxi, Wang, Jinjiang, Chen, Xi, Wu, Minjuan, Ying, Qi-long, Qin, Xingjun, Wang, Yefei, Wang, Yue
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Letter to the Editor
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9229757/
https://www.ncbi.nlm.nih.gov/pubmed/35751058
http://dx.doi.org/10.1186/s12943-022-01604-x
Descripción
Sumario:Cas9 RNA functions as a miRNA sponge. Let-7 is the dominant regulated miRNA by Cas9 RNA. RNA sequence optimization of Cas9 by synonymous mutation improves its safety. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12943-022-01604-x.

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