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Optimization of Cas9 RNA sequence to reduce its unexpected effects as a microRNA sponge
Cas9 RNA functions as a miRNA sponge. Let-7 is the dominant regulated miRNA by Cas9 RNA. RNA sequence optimization of Cas9 by synonymous mutation improves its safety. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12943-022-01604-x.
| Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9229757/ https://www.ncbi.nlm.nih.gov/pubmed/35751058 http://dx.doi.org/10.1186/s12943-022-01604-x |
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| author | Jiang, Junfeng Zeng, Tao Zhang, Li Fan, Xingfei Jin, Qishu Ni, Haitao Ye, Yusheng Cheng, Lipeng Li, Li Wang, Liujun Xu, Sha Yang, Yu Gu, Juan Guo, Bing Wang, Lei Li, Xin Qin, Yingyi Li, Jiaxi Wang, Jinjiang Chen, Xi Wu, Minjuan Ying, Qi-long Qin, Xingjun Wang, Yefei Wang, Yue |
| author_facet | Jiang, Junfeng Zeng, Tao Zhang, Li Fan, Xingfei Jin, Qishu Ni, Haitao Ye, Yusheng Cheng, Lipeng Li, Li Wang, Liujun Xu, Sha Yang, Yu Gu, Juan Guo, Bing Wang, Lei Li, Xin Qin, Yingyi Li, Jiaxi Wang, Jinjiang Chen, Xi Wu, Minjuan Ying, Qi-long Qin, Xingjun Wang, Yefei Wang, Yue |
| author_sort | Jiang, Junfeng |
| collection | PubMed |
| description | Cas9 RNA functions as a miRNA sponge. Let-7 is the dominant regulated miRNA by Cas9 RNA. RNA sequence optimization of Cas9 by synonymous mutation improves its safety. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12943-022-01604-x. |
| format | Online Article Text |
| id | pubmed-9229757 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2022 |
| publisher | BioMed Central |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-92297572022-06-25 Optimization of Cas9 RNA sequence to reduce its unexpected effects as a microRNA sponge Jiang, Junfeng Zeng, Tao Zhang, Li Fan, Xingfei Jin, Qishu Ni, Haitao Ye, Yusheng Cheng, Lipeng Li, Li Wang, Liujun Xu, Sha Yang, Yu Gu, Juan Guo, Bing Wang, Lei Li, Xin Qin, Yingyi Li, Jiaxi Wang, Jinjiang Chen, Xi Wu, Minjuan Ying, Qi-long Qin, Xingjun Wang, Yefei Wang, Yue Mol Cancer Letter to the Editor Cas9 RNA functions as a miRNA sponge. Let-7 is the dominant regulated miRNA by Cas9 RNA. RNA sequence optimization of Cas9 by synonymous mutation improves its safety. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12943-022-01604-x. BioMed Central 2022-06-24 /pmc/articles/PMC9229757/ /pubmed/35751058 http://dx.doi.org/10.1186/s12943-022-01604-x Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
| spellingShingle | Letter to the Editor Jiang, Junfeng Zeng, Tao Zhang, Li Fan, Xingfei Jin, Qishu Ni, Haitao Ye, Yusheng Cheng, Lipeng Li, Li Wang, Liujun Xu, Sha Yang, Yu Gu, Juan Guo, Bing Wang, Lei Li, Xin Qin, Yingyi Li, Jiaxi Wang, Jinjiang Chen, Xi Wu, Minjuan Ying, Qi-long Qin, Xingjun Wang, Yefei Wang, Yue Optimization of Cas9 RNA sequence to reduce its unexpected effects as a microRNA sponge |
| title | Optimization of Cas9 RNA sequence to reduce its unexpected effects as a microRNA sponge |
| title_full | Optimization of Cas9 RNA sequence to reduce its unexpected effects as a microRNA sponge |
| title_fullStr | Optimization of Cas9 RNA sequence to reduce its unexpected effects as a microRNA sponge |
| title_full_unstemmed | Optimization of Cas9 RNA sequence to reduce its unexpected effects as a microRNA sponge |
| title_short | Optimization of Cas9 RNA sequence to reduce its unexpected effects as a microRNA sponge |
| title_sort | optimization of cas9 rna sequence to reduce its unexpected effects as a microrna sponge |
| topic | Letter to the Editor |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9229757/ https://www.ncbi.nlm.nih.gov/pubmed/35751058 http://dx.doi.org/10.1186/s12943-022-01604-x |
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