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Evaluation of the Pathogenic Potential of Escherichia coli Strains Isolated from Eye Infections

While primarily Gram-positive bacteria cause bacterial eye infections, several Gram-negative species also pose eye health risks. Currently, few studies have tried to understand the pathogenic mechanisms involved in E. coli eye infections. Therefore, this study aimed to establish the pathogenic poten...

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Autores principales: Nunes, Pedro Henrique Soares, Valiatti, Tiago Barcelos, Santos, Ana Carolina de Mello, Nascimento, Júllia Assis da Silva, Santos-Neto, José Francisco, Rocchetti, Talita Trevizani, Yu, Maria Cecilia Zorat, Hofling-Lima, Ana Luisa, Gomes, Tânia Aparecida Tardelli
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9229993/
https://www.ncbi.nlm.nih.gov/pubmed/35744602
http://dx.doi.org/10.3390/microorganisms10061084
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author Nunes, Pedro Henrique Soares
Valiatti, Tiago Barcelos
Santos, Ana Carolina de Mello
Nascimento, Júllia Assis da Silva
Santos-Neto, José Francisco
Rocchetti, Talita Trevizani
Yu, Maria Cecilia Zorat
Hofling-Lima, Ana Luisa
Gomes, Tânia Aparecida Tardelli
author_facet Nunes, Pedro Henrique Soares
Valiatti, Tiago Barcelos
Santos, Ana Carolina de Mello
Nascimento, Júllia Assis da Silva
Santos-Neto, José Francisco
Rocchetti, Talita Trevizani
Yu, Maria Cecilia Zorat
Hofling-Lima, Ana Luisa
Gomes, Tânia Aparecida Tardelli
author_sort Nunes, Pedro Henrique Soares
collection PubMed
description While primarily Gram-positive bacteria cause bacterial eye infections, several Gram-negative species also pose eye health risks. Currently, few studies have tried to understand the pathogenic mechanisms involved in E. coli eye infections. Therefore, this study aimed to establish the pathogenic potential of E. coli strains isolated from eye infections. Twenty-two strains isolated between 2005 and 2019 from patients with keratitis or conjunctivitis were included and submitted to traditional polymerase chain reactions (PCR) to define their virulence profile, phylogeny, clonal relationship, and sequence type (ST). Phenotypic assays were employed to determine hemolytic activity, antimicrobial susceptibility, and adhesion to human primary corneal epithelial cells (PCS-700-010). The phylogenetic results indicated that groups B2 and ST131 were the most frequent. Twenty-five virulence genes were found among our strains, with ecp, sitA, fimA, and fyuA being the most prevalent. Two strains presented a hemolytic phenotype, and resistance to ciprofloxacin and ertapenem was found in six strains and one strain, respectively. Regarding adherence, all but one strains adhered in vitro to corneal cells. Our results indicate significant genetic and virulence variation among ocular strains and point to an ocular pathogenic potential related to multiple virulence mechanisms.
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spelling pubmed-92299932022-06-25 Evaluation of the Pathogenic Potential of Escherichia coli Strains Isolated from Eye Infections Nunes, Pedro Henrique Soares Valiatti, Tiago Barcelos Santos, Ana Carolina de Mello Nascimento, Júllia Assis da Silva Santos-Neto, José Francisco Rocchetti, Talita Trevizani Yu, Maria Cecilia Zorat Hofling-Lima, Ana Luisa Gomes, Tânia Aparecida Tardelli Microorganisms Article While primarily Gram-positive bacteria cause bacterial eye infections, several Gram-negative species also pose eye health risks. Currently, few studies have tried to understand the pathogenic mechanisms involved in E. coli eye infections. Therefore, this study aimed to establish the pathogenic potential of E. coli strains isolated from eye infections. Twenty-two strains isolated between 2005 and 2019 from patients with keratitis or conjunctivitis were included and submitted to traditional polymerase chain reactions (PCR) to define their virulence profile, phylogeny, clonal relationship, and sequence type (ST). Phenotypic assays were employed to determine hemolytic activity, antimicrobial susceptibility, and adhesion to human primary corneal epithelial cells (PCS-700-010). The phylogenetic results indicated that groups B2 and ST131 were the most frequent. Twenty-five virulence genes were found among our strains, with ecp, sitA, fimA, and fyuA being the most prevalent. Two strains presented a hemolytic phenotype, and resistance to ciprofloxacin and ertapenem was found in six strains and one strain, respectively. Regarding adherence, all but one strains adhered in vitro to corneal cells. Our results indicate significant genetic and virulence variation among ocular strains and point to an ocular pathogenic potential related to multiple virulence mechanisms. MDPI 2022-05-25 /pmc/articles/PMC9229993/ /pubmed/35744602 http://dx.doi.org/10.3390/microorganisms10061084 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Nunes, Pedro Henrique Soares
Valiatti, Tiago Barcelos
Santos, Ana Carolina de Mello
Nascimento, Júllia Assis da Silva
Santos-Neto, José Francisco
Rocchetti, Talita Trevizani
Yu, Maria Cecilia Zorat
Hofling-Lima, Ana Luisa
Gomes, Tânia Aparecida Tardelli
Evaluation of the Pathogenic Potential of Escherichia coli Strains Isolated from Eye Infections
title Evaluation of the Pathogenic Potential of Escherichia coli Strains Isolated from Eye Infections
title_full Evaluation of the Pathogenic Potential of Escherichia coli Strains Isolated from Eye Infections
title_fullStr Evaluation of the Pathogenic Potential of Escherichia coli Strains Isolated from Eye Infections
title_full_unstemmed Evaluation of the Pathogenic Potential of Escherichia coli Strains Isolated from Eye Infections
title_short Evaluation of the Pathogenic Potential of Escherichia coli Strains Isolated from Eye Infections
title_sort evaluation of the pathogenic potential of escherichia coli strains isolated from eye infections
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9229993/
https://www.ncbi.nlm.nih.gov/pubmed/35744602
http://dx.doi.org/10.3390/microorganisms10061084
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