Cargando…

Lack of Direct Correlation between Biofilm Formation and Antimicrobial Resistance in Clinical Staphylococcus epidermidis Isolates from an Italian Hospital

Staphylococcus epidermidis is an opportunistic pathogen and a frequent cause of nosocomial infections. In this work, we show that, among 51 S. epidermidis isolates from an Italian hospital, only a minority displayed biofilm formation, regardless of their isolation source (peripheral blood, catheter,...

Descripción completa

Detalles Bibliográficos
Autores principales: Carcione, Davide, Leccese, Gabriella, Conte, Gianmarco, Rossi, Elio, Intra, Jari, Bonomi, Alice, Sabella, Simona, Moreo, Massimo, Landini, Paolo, Brilli, Matteo, Paroni, Moira
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9230108/
https://www.ncbi.nlm.nih.gov/pubmed/35744681
http://dx.doi.org/10.3390/microorganisms10061163
_version_ 1784734978857238528
author Carcione, Davide
Leccese, Gabriella
Conte, Gianmarco
Rossi, Elio
Intra, Jari
Bonomi, Alice
Sabella, Simona
Moreo, Massimo
Landini, Paolo
Brilli, Matteo
Paroni, Moira
author_facet Carcione, Davide
Leccese, Gabriella
Conte, Gianmarco
Rossi, Elio
Intra, Jari
Bonomi, Alice
Sabella, Simona
Moreo, Massimo
Landini, Paolo
Brilli, Matteo
Paroni, Moira
author_sort Carcione, Davide
collection PubMed
description Staphylococcus epidermidis is an opportunistic pathogen and a frequent cause of nosocomial infections. In this work, we show that, among 51 S. epidermidis isolates from an Italian hospital, only a minority displayed biofilm formation, regardless of their isolation source (peripheral blood, catheter, or skin wounds); however, among the biofilm-producing isolates, those from catheters were the most efficient in biofilm formation. Interestingly, most isolates including strong biofilm producers displayed production levels of PIA (polysaccharide intercellular adhesin), the main S. epidermidis extracellular polysaccharide, similar to reference S. epidermidis strains classified as non-biofilm formers, and much lower than those classified as intermediate or high biofilm formers, possibly suggesting that high levels of PIA production do not confer a particular advantage for clinical isolates. Finally, while for the reference S. epidermidis strains the biofilm production clearly correlated with the decreased sensitivity to antibiotics, in particular, protein synthesis inhibitors, in our clinical isolates, such positive correlation was limited to tetracycline. In contrast, we observed an inverse correlation between biofilm formation and the minimal inhibitory concentrations for levofloxacin and teicoplanin. In addition, in growth conditions favoring PIA production, the biofilm-forming isolates showed increased sensitivity to daptomycin, clindamycin, and erythromycin, with increased tolerance to the trimethoprim/sulfamethoxazole association. The lack of direct correlation between the biofilm production and increased tolerance to antibiotics in S. epidermidis isolates from a clinical setting would suggest, at least for some antimicrobials, the possible existence of a trade-off between the production of biofilm determinants and antibiotic resistance.
format Online
Article
Text
id pubmed-9230108
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-92301082022-06-25 Lack of Direct Correlation between Biofilm Formation and Antimicrobial Resistance in Clinical Staphylococcus epidermidis Isolates from an Italian Hospital Carcione, Davide Leccese, Gabriella Conte, Gianmarco Rossi, Elio Intra, Jari Bonomi, Alice Sabella, Simona Moreo, Massimo Landini, Paolo Brilli, Matteo Paroni, Moira Microorganisms Article Staphylococcus epidermidis is an opportunistic pathogen and a frequent cause of nosocomial infections. In this work, we show that, among 51 S. epidermidis isolates from an Italian hospital, only a minority displayed biofilm formation, regardless of their isolation source (peripheral blood, catheter, or skin wounds); however, among the biofilm-producing isolates, those from catheters were the most efficient in biofilm formation. Interestingly, most isolates including strong biofilm producers displayed production levels of PIA (polysaccharide intercellular adhesin), the main S. epidermidis extracellular polysaccharide, similar to reference S. epidermidis strains classified as non-biofilm formers, and much lower than those classified as intermediate or high biofilm formers, possibly suggesting that high levels of PIA production do not confer a particular advantage for clinical isolates. Finally, while for the reference S. epidermidis strains the biofilm production clearly correlated with the decreased sensitivity to antibiotics, in particular, protein synthesis inhibitors, in our clinical isolates, such positive correlation was limited to tetracycline. In contrast, we observed an inverse correlation between biofilm formation and the minimal inhibitory concentrations for levofloxacin and teicoplanin. In addition, in growth conditions favoring PIA production, the biofilm-forming isolates showed increased sensitivity to daptomycin, clindamycin, and erythromycin, with increased tolerance to the trimethoprim/sulfamethoxazole association. The lack of direct correlation between the biofilm production and increased tolerance to antibiotics in S. epidermidis isolates from a clinical setting would suggest, at least for some antimicrobials, the possible existence of a trade-off between the production of biofilm determinants and antibiotic resistance. MDPI 2022-06-06 /pmc/articles/PMC9230108/ /pubmed/35744681 http://dx.doi.org/10.3390/microorganisms10061163 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Carcione, Davide
Leccese, Gabriella
Conte, Gianmarco
Rossi, Elio
Intra, Jari
Bonomi, Alice
Sabella, Simona
Moreo, Massimo
Landini, Paolo
Brilli, Matteo
Paroni, Moira
Lack of Direct Correlation between Biofilm Formation and Antimicrobial Resistance in Clinical Staphylococcus epidermidis Isolates from an Italian Hospital
title Lack of Direct Correlation between Biofilm Formation and Antimicrobial Resistance in Clinical Staphylococcus epidermidis Isolates from an Italian Hospital
title_full Lack of Direct Correlation between Biofilm Formation and Antimicrobial Resistance in Clinical Staphylococcus epidermidis Isolates from an Italian Hospital
title_fullStr Lack of Direct Correlation between Biofilm Formation and Antimicrobial Resistance in Clinical Staphylococcus epidermidis Isolates from an Italian Hospital
title_full_unstemmed Lack of Direct Correlation between Biofilm Formation and Antimicrobial Resistance in Clinical Staphylococcus epidermidis Isolates from an Italian Hospital
title_short Lack of Direct Correlation between Biofilm Formation and Antimicrobial Resistance in Clinical Staphylococcus epidermidis Isolates from an Italian Hospital
title_sort lack of direct correlation between biofilm formation and antimicrobial resistance in clinical staphylococcus epidermidis isolates from an italian hospital
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9230108/
https://www.ncbi.nlm.nih.gov/pubmed/35744681
http://dx.doi.org/10.3390/microorganisms10061163
work_keys_str_mv AT carcionedavide lackofdirectcorrelationbetweenbiofilmformationandantimicrobialresistanceinclinicalstaphylococcusepidermidisisolatesfromanitalianhospital
AT leccesegabriella lackofdirectcorrelationbetweenbiofilmformationandantimicrobialresistanceinclinicalstaphylococcusepidermidisisolatesfromanitalianhospital
AT contegianmarco lackofdirectcorrelationbetweenbiofilmformationandantimicrobialresistanceinclinicalstaphylococcusepidermidisisolatesfromanitalianhospital
AT rossielio lackofdirectcorrelationbetweenbiofilmformationandantimicrobialresistanceinclinicalstaphylococcusepidermidisisolatesfromanitalianhospital
AT intrajari lackofdirectcorrelationbetweenbiofilmformationandantimicrobialresistanceinclinicalstaphylococcusepidermidisisolatesfromanitalianhospital
AT bonomialice lackofdirectcorrelationbetweenbiofilmformationandantimicrobialresistanceinclinicalstaphylococcusepidermidisisolatesfromanitalianhospital
AT sabellasimona lackofdirectcorrelationbetweenbiofilmformationandantimicrobialresistanceinclinicalstaphylococcusepidermidisisolatesfromanitalianhospital
AT moreomassimo lackofdirectcorrelationbetweenbiofilmformationandantimicrobialresistanceinclinicalstaphylococcusepidermidisisolatesfromanitalianhospital
AT landinipaolo lackofdirectcorrelationbetweenbiofilmformationandantimicrobialresistanceinclinicalstaphylococcusepidermidisisolatesfromanitalianhospital
AT brillimatteo lackofdirectcorrelationbetweenbiofilmformationandantimicrobialresistanceinclinicalstaphylococcusepidermidisisolatesfromanitalianhospital
AT paronimoira lackofdirectcorrelationbetweenbiofilmformationandantimicrobialresistanceinclinicalstaphylococcusepidermidisisolatesfromanitalianhospital