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Strategies That Facilitate Extraction-Free SARS-CoV-2 Nucleic Acid Amplification Tests
The COVID-19 pandemic has resulted in an unprecedented global demand for in vitro diagnostic reagents. Supply shortages and hoarding have impacted testing capacity which has led to inefficient COVID-19 case identification and transmission control, predominantly in developing countries. Traditionally...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9230587/ https://www.ncbi.nlm.nih.gov/pubmed/35746782 http://dx.doi.org/10.3390/v14061311 |
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author | Delgado-Diaz, David J. Sakthivel, Dhanasekaran Nguyen, Hanh H. T. Farrokzhad, Khashayar Hopper, William Narh, Charles A. Richards, Jack S. |
author_facet | Delgado-Diaz, David J. Sakthivel, Dhanasekaran Nguyen, Hanh H. T. Farrokzhad, Khashayar Hopper, William Narh, Charles A. Richards, Jack S. |
author_sort | Delgado-Diaz, David J. |
collection | PubMed |
description | The COVID-19 pandemic has resulted in an unprecedented global demand for in vitro diagnostic reagents. Supply shortages and hoarding have impacted testing capacity which has led to inefficient COVID-19 case identification and transmission control, predominantly in developing countries. Traditionally, RNA extraction is a prerequisite for conducting SARS-CoV-2 nucleic acid amplification tests (NAAT); however, simplified methods of sample processing have been successful at bypassing typical nucleic acid extraction steps, enabling extraction-free SARS-CoV-2 NAAT workflows. These methods involve chemical and physical approaches that are inexpensive and easily accessible alternatives to overcome extraction kit supply shortages, while offering acceptable test performance. Here we provide an overview of three main sample preparation strategies that have been shown to facilitate extraction-free SARS-CoV-2 NAATs. |
format | Online Article Text |
id | pubmed-9230587 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-92305872022-06-25 Strategies That Facilitate Extraction-Free SARS-CoV-2 Nucleic Acid Amplification Tests Delgado-Diaz, David J. Sakthivel, Dhanasekaran Nguyen, Hanh H. T. Farrokzhad, Khashayar Hopper, William Narh, Charles A. Richards, Jack S. Viruses Review The COVID-19 pandemic has resulted in an unprecedented global demand for in vitro diagnostic reagents. Supply shortages and hoarding have impacted testing capacity which has led to inefficient COVID-19 case identification and transmission control, predominantly in developing countries. Traditionally, RNA extraction is a prerequisite for conducting SARS-CoV-2 nucleic acid amplification tests (NAAT); however, simplified methods of sample processing have been successful at bypassing typical nucleic acid extraction steps, enabling extraction-free SARS-CoV-2 NAAT workflows. These methods involve chemical and physical approaches that are inexpensive and easily accessible alternatives to overcome extraction kit supply shortages, while offering acceptable test performance. Here we provide an overview of three main sample preparation strategies that have been shown to facilitate extraction-free SARS-CoV-2 NAATs. MDPI 2022-06-15 /pmc/articles/PMC9230587/ /pubmed/35746782 http://dx.doi.org/10.3390/v14061311 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Delgado-Diaz, David J. Sakthivel, Dhanasekaran Nguyen, Hanh H. T. Farrokzhad, Khashayar Hopper, William Narh, Charles A. Richards, Jack S. Strategies That Facilitate Extraction-Free SARS-CoV-2 Nucleic Acid Amplification Tests |
title | Strategies That Facilitate Extraction-Free SARS-CoV-2 Nucleic Acid Amplification Tests |
title_full | Strategies That Facilitate Extraction-Free SARS-CoV-2 Nucleic Acid Amplification Tests |
title_fullStr | Strategies That Facilitate Extraction-Free SARS-CoV-2 Nucleic Acid Amplification Tests |
title_full_unstemmed | Strategies That Facilitate Extraction-Free SARS-CoV-2 Nucleic Acid Amplification Tests |
title_short | Strategies That Facilitate Extraction-Free SARS-CoV-2 Nucleic Acid Amplification Tests |
title_sort | strategies that facilitate extraction-free sars-cov-2 nucleic acid amplification tests |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9230587/ https://www.ncbi.nlm.nih.gov/pubmed/35746782 http://dx.doi.org/10.3390/v14061311 |
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