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Viromes of Hungarian Peach Trees Identified by High-Throughput Sequencing of Small RNAs

Peach trees can be infected with viruses and viroids. As we do not have efficient plant protection methods against these pathogens, the prevention of infection is crucial. Fruit trees are maintained by vegetative propagation. Planting material such as certified mother trees and rootstocks should be...

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Autores principales: Barath, Daniel, Jaksa-Czotter, Nikoletta, Varga, Tunde, Varallyay, Eva
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9230589/
https://www.ncbi.nlm.nih.gov/pubmed/35736743
http://dx.doi.org/10.3390/plants11121591
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author Barath, Daniel
Jaksa-Czotter, Nikoletta
Varga, Tunde
Varallyay, Eva
author_facet Barath, Daniel
Jaksa-Czotter, Nikoletta
Varga, Tunde
Varallyay, Eva
author_sort Barath, Daniel
collection PubMed
description Peach trees can be infected with viruses and viroids. As we do not have efficient plant protection methods against these pathogens, the prevention of infection is crucial. Fruit trees are maintained by vegetative propagation. Planting material such as certified mother trees and rootstocks should be free from viruses and viroids, and this status has to be regularly checked to prevent infections. We surveyed certified peach trees for the presence of viruses and viroids using small RNA high-throughput sequencing (HTS), an unbiased virus diagnostic method. The results of the bioinformatic analysis of HTS were validated by other molecular methods including RT-PCR, Northern blot hybridization and loop-mediated isothermal amplification (LAMP). We found the presence of plum pox virus and peach latent mosaic viroid (PLMVd) in the vector-free isolator houses, whose presence should be regularly tested. Moreover, we detected frequent infection with recently described viruses such as nectarine stem pitting-associated virus and peach-associated luteovirus (PaLV). During the survey, PLMVd and PaLV were detected for the first time in Hungary. The analysis of the presenting virus variants and possible sources of infection suggests that the source of the viral infection could be the infected propagating material. Our study emphasizes the importance of using sensitive and trustworthy diagnostic techniques to be able to detect viral infections and successfully prevent their spread by propagation material.
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spelling pubmed-92305892022-06-25 Viromes of Hungarian Peach Trees Identified by High-Throughput Sequencing of Small RNAs Barath, Daniel Jaksa-Czotter, Nikoletta Varga, Tunde Varallyay, Eva Plants (Basel) Article Peach trees can be infected with viruses and viroids. As we do not have efficient plant protection methods against these pathogens, the prevention of infection is crucial. Fruit trees are maintained by vegetative propagation. Planting material such as certified mother trees and rootstocks should be free from viruses and viroids, and this status has to be regularly checked to prevent infections. We surveyed certified peach trees for the presence of viruses and viroids using small RNA high-throughput sequencing (HTS), an unbiased virus diagnostic method. The results of the bioinformatic analysis of HTS were validated by other molecular methods including RT-PCR, Northern blot hybridization and loop-mediated isothermal amplification (LAMP). We found the presence of plum pox virus and peach latent mosaic viroid (PLMVd) in the vector-free isolator houses, whose presence should be regularly tested. Moreover, we detected frequent infection with recently described viruses such as nectarine stem pitting-associated virus and peach-associated luteovirus (PaLV). During the survey, PLMVd and PaLV were detected for the first time in Hungary. The analysis of the presenting virus variants and possible sources of infection suggests that the source of the viral infection could be the infected propagating material. Our study emphasizes the importance of using sensitive and trustworthy diagnostic techniques to be able to detect viral infections and successfully prevent their spread by propagation material. MDPI 2022-06-16 /pmc/articles/PMC9230589/ /pubmed/35736743 http://dx.doi.org/10.3390/plants11121591 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Barath, Daniel
Jaksa-Czotter, Nikoletta
Varga, Tunde
Varallyay, Eva
Viromes of Hungarian Peach Trees Identified by High-Throughput Sequencing of Small RNAs
title Viromes of Hungarian Peach Trees Identified by High-Throughput Sequencing of Small RNAs
title_full Viromes of Hungarian Peach Trees Identified by High-Throughput Sequencing of Small RNAs
title_fullStr Viromes of Hungarian Peach Trees Identified by High-Throughput Sequencing of Small RNAs
title_full_unstemmed Viromes of Hungarian Peach Trees Identified by High-Throughput Sequencing of Small RNAs
title_short Viromes of Hungarian Peach Trees Identified by High-Throughput Sequencing of Small RNAs
title_sort viromes of hungarian peach trees identified by high-throughput sequencing of small rnas
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9230589/
https://www.ncbi.nlm.nih.gov/pubmed/35736743
http://dx.doi.org/10.3390/plants11121591
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