Cargando…

Cell Immobilization Using Alginate-Based Beads as a Protective Technique against Stressful Conditions of Hydrolysates for 2G Ethanol Production

The development of biorefineries brings the necessity of an efficient consumption of all sugars released from biomasses, including xylose. In addition, the presence of inhibitors in biomass hydrolysates is one of the main challenges in bioprocess feasibility. In this study, the application of Ca-alg...

Descripción completa

Detalles Bibliográficos
Autores principales: Soares, Raiane C., Zangirolami, Teresa C., Giordano, Raquel L. C., Demeke, Mekonnen M., Thevelein, Johan M., Milessi, Thais S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9230679/
https://www.ncbi.nlm.nih.gov/pubmed/35745976
http://dx.doi.org/10.3390/polym14122400
_version_ 1784735125426143232
author Soares, Raiane C.
Zangirolami, Teresa C.
Giordano, Raquel L. C.
Demeke, Mekonnen M.
Thevelein, Johan M.
Milessi, Thais S.
author_facet Soares, Raiane C.
Zangirolami, Teresa C.
Giordano, Raquel L. C.
Demeke, Mekonnen M.
Thevelein, Johan M.
Milessi, Thais S.
author_sort Soares, Raiane C.
collection PubMed
description The development of biorefineries brings the necessity of an efficient consumption of all sugars released from biomasses, including xylose. In addition, the presence of inhibitors in biomass hydrolysates is one of the main challenges in bioprocess feasibility. In this study, the application of Ca-alginate hybrid gels in the immobilization of xylose-consuming recombinant yeast was explored with the aim of improving the tolerance of inhibitors. The recombinant yeast Saccharomyces cerevisiae GSE16-T18SI.1 (T18) was immobilized in Ca-alginate and Ca-alginate–chitosan hybrid beads, and its performance on xylose fermentation was evaluated in terms of tolerance to different acetic acid concentrations (0–12 g/L) and repeated batches of crude sugarcane bagasse hemicellulose hydrolysate. The use of the hybrid gel improved yeast performance in the presence of 12 g/L of acetic acid, achieving 1.13 g/L/h of productivity and reaching 75% of the theoretical ethanol yield, with an improvement of 32% in the xylose consumption rate (1:1 V(beads)/V(medium), 35 °C, 150 rpm and pH 5.2). The use of hybrid alginate–chitosan gel also led to better yeast performance at crude hydrolysate, yielding one more batch than the pure-alginate beads. These results demonstrate the potential of a hybrid gel as an approach that could increase 2G ethanol productivity and allow cell recycling for a longer period.
format Online
Article
Text
id pubmed-9230679
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-92306792022-06-25 Cell Immobilization Using Alginate-Based Beads as a Protective Technique against Stressful Conditions of Hydrolysates for 2G Ethanol Production Soares, Raiane C. Zangirolami, Teresa C. Giordano, Raquel L. C. Demeke, Mekonnen M. Thevelein, Johan M. Milessi, Thais S. Polymers (Basel) Article The development of biorefineries brings the necessity of an efficient consumption of all sugars released from biomasses, including xylose. In addition, the presence of inhibitors in biomass hydrolysates is one of the main challenges in bioprocess feasibility. In this study, the application of Ca-alginate hybrid gels in the immobilization of xylose-consuming recombinant yeast was explored with the aim of improving the tolerance of inhibitors. The recombinant yeast Saccharomyces cerevisiae GSE16-T18SI.1 (T18) was immobilized in Ca-alginate and Ca-alginate–chitosan hybrid beads, and its performance on xylose fermentation was evaluated in terms of tolerance to different acetic acid concentrations (0–12 g/L) and repeated batches of crude sugarcane bagasse hemicellulose hydrolysate. The use of the hybrid gel improved yeast performance in the presence of 12 g/L of acetic acid, achieving 1.13 g/L/h of productivity and reaching 75% of the theoretical ethanol yield, with an improvement of 32% in the xylose consumption rate (1:1 V(beads)/V(medium), 35 °C, 150 rpm and pH 5.2). The use of hybrid alginate–chitosan gel also led to better yeast performance at crude hydrolysate, yielding one more batch than the pure-alginate beads. These results demonstrate the potential of a hybrid gel as an approach that could increase 2G ethanol productivity and allow cell recycling for a longer period. MDPI 2022-06-14 /pmc/articles/PMC9230679/ /pubmed/35745976 http://dx.doi.org/10.3390/polym14122400 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Soares, Raiane C.
Zangirolami, Teresa C.
Giordano, Raquel L. C.
Demeke, Mekonnen M.
Thevelein, Johan M.
Milessi, Thais S.
Cell Immobilization Using Alginate-Based Beads as a Protective Technique against Stressful Conditions of Hydrolysates for 2G Ethanol Production
title Cell Immobilization Using Alginate-Based Beads as a Protective Technique against Stressful Conditions of Hydrolysates for 2G Ethanol Production
title_full Cell Immobilization Using Alginate-Based Beads as a Protective Technique against Stressful Conditions of Hydrolysates for 2G Ethanol Production
title_fullStr Cell Immobilization Using Alginate-Based Beads as a Protective Technique against Stressful Conditions of Hydrolysates for 2G Ethanol Production
title_full_unstemmed Cell Immobilization Using Alginate-Based Beads as a Protective Technique against Stressful Conditions of Hydrolysates for 2G Ethanol Production
title_short Cell Immobilization Using Alginate-Based Beads as a Protective Technique against Stressful Conditions of Hydrolysates for 2G Ethanol Production
title_sort cell immobilization using alginate-based beads as a protective technique against stressful conditions of hydrolysates for 2g ethanol production
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9230679/
https://www.ncbi.nlm.nih.gov/pubmed/35745976
http://dx.doi.org/10.3390/polym14122400
work_keys_str_mv AT soaresraianec cellimmobilizationusingalginatebasedbeadsasaprotectivetechniqueagainststressfulconditionsofhydrolysatesfor2gethanolproduction
AT zangirolamiteresac cellimmobilizationusingalginatebasedbeadsasaprotectivetechniqueagainststressfulconditionsofhydrolysatesfor2gethanolproduction
AT giordanoraquellc cellimmobilizationusingalginatebasedbeadsasaprotectivetechniqueagainststressfulconditionsofhydrolysatesfor2gethanolproduction
AT demekemekonnenm cellimmobilizationusingalginatebasedbeadsasaprotectivetechniqueagainststressfulconditionsofhydrolysatesfor2gethanolproduction
AT theveleinjohanm cellimmobilizationusingalginatebasedbeadsasaprotectivetechniqueagainststressfulconditionsofhydrolysatesfor2gethanolproduction
AT milessithaiss cellimmobilizationusingalginatebasedbeadsasaprotectivetechniqueagainststressfulconditionsofhydrolysatesfor2gethanolproduction