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A Glycolipid α-GalCer Derivative, 7DW8-5 as a Novel Mucosal Adjuvant for the Split Inactivated Influenza Vaccine

Influenza virus infects the host and transmits through the respiratory tract (i.e., the mouth and nose); therefore, the development of intranasal influenza vaccines that mimic the natural infection, coupled with an efficient mucosal adjuvant, is an attractive alternative to current parenteral vaccin...

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Autores principales: Feng, Huapeng, Sun, Ruolin, Song, Guanru, Zhu, Shunfan, Nie, Zhenyu, Lin, Liming, Yi, Ruonan, Wu, Shixiang, Wang, Genzhu, He, Yulong, Wang, Siquan, Wang, Pei, Wu, Li, Shu, Jianhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9230830/
https://www.ncbi.nlm.nih.gov/pubmed/35746644
http://dx.doi.org/10.3390/v14061174
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author Feng, Huapeng
Sun, Ruolin
Song, Guanru
Zhu, Shunfan
Nie, Zhenyu
Lin, Liming
Yi, Ruonan
Wu, Shixiang
Wang, Genzhu
He, Yulong
Wang, Siquan
Wang, Pei
Wu, Li
Shu, Jianhong
author_facet Feng, Huapeng
Sun, Ruolin
Song, Guanru
Zhu, Shunfan
Nie, Zhenyu
Lin, Liming
Yi, Ruonan
Wu, Shixiang
Wang, Genzhu
He, Yulong
Wang, Siquan
Wang, Pei
Wu, Li
Shu, Jianhong
author_sort Feng, Huapeng
collection PubMed
description Influenza virus infects the host and transmits through the respiratory tract (i.e., the mouth and nose); therefore, the development of intranasal influenza vaccines that mimic the natural infection, coupled with an efficient mucosal adjuvant, is an attractive alternative to current parenteral vaccines. However, with the withdrawal of cholera toxin and Escherichia coli heat-labile endotoxin from clinical use due to side effects, there are no approved adjuvants for intranasal vaccines. Therefore, safe and effective mucosal adjuvants are urgently needed. Previously, we reported that one derivative of α-Galactosylceramide (α-GalCer), 7DW8-5, could enhance the protective efficacy of split influenza vaccine by injection administration. However, the mucosal adjuvanticity of 7DW8-5 is still unclear. In this study, we found that 7DW8-5 promotes the production of secret IgA antibodies and IgG antibodies and enhances the protective efficacy of the split influenza vaccine by intranasal administration. Furthermore, co-administration of 7DW8-5 with the split influenza vaccine significantly reduces the virus shedding in the upper and lower respiratory tract after lethal challenge. Our results demonstrate that 7DW8-5 is a novel mucosal adjuvant for the split influenza vaccine.
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spelling pubmed-92308302022-06-25 A Glycolipid α-GalCer Derivative, 7DW8-5 as a Novel Mucosal Adjuvant for the Split Inactivated Influenza Vaccine Feng, Huapeng Sun, Ruolin Song, Guanru Zhu, Shunfan Nie, Zhenyu Lin, Liming Yi, Ruonan Wu, Shixiang Wang, Genzhu He, Yulong Wang, Siquan Wang, Pei Wu, Li Shu, Jianhong Viruses Article Influenza virus infects the host and transmits through the respiratory tract (i.e., the mouth and nose); therefore, the development of intranasal influenza vaccines that mimic the natural infection, coupled with an efficient mucosal adjuvant, is an attractive alternative to current parenteral vaccines. However, with the withdrawal of cholera toxin and Escherichia coli heat-labile endotoxin from clinical use due to side effects, there are no approved adjuvants for intranasal vaccines. Therefore, safe and effective mucosal adjuvants are urgently needed. Previously, we reported that one derivative of α-Galactosylceramide (α-GalCer), 7DW8-5, could enhance the protective efficacy of split influenza vaccine by injection administration. However, the mucosal adjuvanticity of 7DW8-5 is still unclear. In this study, we found that 7DW8-5 promotes the production of secret IgA antibodies and IgG antibodies and enhances the protective efficacy of the split influenza vaccine by intranasal administration. Furthermore, co-administration of 7DW8-5 with the split influenza vaccine significantly reduces the virus shedding in the upper and lower respiratory tract after lethal challenge. Our results demonstrate that 7DW8-5 is a novel mucosal adjuvant for the split influenza vaccine. MDPI 2022-05-28 /pmc/articles/PMC9230830/ /pubmed/35746644 http://dx.doi.org/10.3390/v14061174 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Feng, Huapeng
Sun, Ruolin
Song, Guanru
Zhu, Shunfan
Nie, Zhenyu
Lin, Liming
Yi, Ruonan
Wu, Shixiang
Wang, Genzhu
He, Yulong
Wang, Siquan
Wang, Pei
Wu, Li
Shu, Jianhong
A Glycolipid α-GalCer Derivative, 7DW8-5 as a Novel Mucosal Adjuvant for the Split Inactivated Influenza Vaccine
title A Glycolipid α-GalCer Derivative, 7DW8-5 as a Novel Mucosal Adjuvant for the Split Inactivated Influenza Vaccine
title_full A Glycolipid α-GalCer Derivative, 7DW8-5 as a Novel Mucosal Adjuvant for the Split Inactivated Influenza Vaccine
title_fullStr A Glycolipid α-GalCer Derivative, 7DW8-5 as a Novel Mucosal Adjuvant for the Split Inactivated Influenza Vaccine
title_full_unstemmed A Glycolipid α-GalCer Derivative, 7DW8-5 as a Novel Mucosal Adjuvant for the Split Inactivated Influenza Vaccine
title_short A Glycolipid α-GalCer Derivative, 7DW8-5 as a Novel Mucosal Adjuvant for the Split Inactivated Influenza Vaccine
title_sort glycolipid α-galcer derivative, 7dw8-5 as a novel mucosal adjuvant for the split inactivated influenza vaccine
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9230830/
https://www.ncbi.nlm.nih.gov/pubmed/35746644
http://dx.doi.org/10.3390/v14061174
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