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Silver Nanoparticles Containing Fucoidan Synthesized by Green Method Have Anti-Trypanosoma cruzi Activity

The brown seaweed Spatoglossum schröederi synthesizes three bioactive fucoidans, the most abundant of which is fucan A. This fucoidan was extracted and its identity was confirmed by chemical analysis, Fourier-transform infrared spectroscopy (FTIR), and agarose gel electrophoresis. Thereafter, silver...

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Autores principales: Souza, Adriana Oliveira, Oliveira, Johny Wysllas de Freitas, Moreno, Claudia Jéssica Gonsalves, de Medeiros, Mayra Jane Campos, Fernandes-Negreiros, Marília Medeiros, Souza, Flavia Roberta Monteiro, Pontes, Daniel Lima, Silva, Marcelo Sousa, Rocha, Hugo Alexandre Oliveira
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9231105/
https://www.ncbi.nlm.nih.gov/pubmed/35745396
http://dx.doi.org/10.3390/nano12122059
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author Souza, Adriana Oliveira
Oliveira, Johny Wysllas de Freitas
Moreno, Claudia Jéssica Gonsalves
de Medeiros, Mayra Jane Campos
Fernandes-Negreiros, Marília Medeiros
Souza, Flavia Roberta Monteiro
Pontes, Daniel Lima
Silva, Marcelo Sousa
Rocha, Hugo Alexandre Oliveira
author_facet Souza, Adriana Oliveira
Oliveira, Johny Wysllas de Freitas
Moreno, Claudia Jéssica Gonsalves
de Medeiros, Mayra Jane Campos
Fernandes-Negreiros, Marília Medeiros
Souza, Flavia Roberta Monteiro
Pontes, Daniel Lima
Silva, Marcelo Sousa
Rocha, Hugo Alexandre Oliveira
author_sort Souza, Adriana Oliveira
collection PubMed
description The brown seaweed Spatoglossum schröederi synthesizes three bioactive fucoidans, the most abundant of which is fucan A. This fucoidan was extracted and its identity was confirmed by chemical analysis, Fourier-transform infrared spectroscopy (FTIR), and agarose gel electrophoresis. Thereafter, silver nanoparticles containing fucan A (AgFuc) were produced using an environmentally friendly synthesis method. AgFuc synthesis was analyzed via UV–vis spectroscopy and FTIR, which confirmed the presence of both silver and fucan A in the AgFuc product. Dynamic light scattering (DLS), X-ray diffraction, scanning electron microscopy, and atomic force microscopy revealed that the AgFuc particles were ~180.0 nm in size and spherical in shape. DLS further demonstrated that AgFuc was stable for five months. Coupled plasma optical emission spectrometry showed that the AgFuc particles contained 5% silver and 95% sugar. AgFuc was shown to be more effective in inhibiting the ability of parasites to reduce MTT than fucan A or silver, regardless of treatment time. In addition, AgFuc induced the death of ~60% of parasites by necrosis and ~17% by apoptosis. Therefore, AgFuc induces damage to the parasites’ mitochondria, which suggests that it is an anti-Trypanosoma cruzi agent. This is the first study to analyze silver nanoparticles containing fucan as an anti-Trypanosoma cruzi agent. Our data indicate that AgFuc nanoparticles have potential therapeutic applications, which should be determined via preclinical in vitro and in vivo studies.
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spelling pubmed-92311052022-06-25 Silver Nanoparticles Containing Fucoidan Synthesized by Green Method Have Anti-Trypanosoma cruzi Activity Souza, Adriana Oliveira Oliveira, Johny Wysllas de Freitas Moreno, Claudia Jéssica Gonsalves de Medeiros, Mayra Jane Campos Fernandes-Negreiros, Marília Medeiros Souza, Flavia Roberta Monteiro Pontes, Daniel Lima Silva, Marcelo Sousa Rocha, Hugo Alexandre Oliveira Nanomaterials (Basel) Article The brown seaweed Spatoglossum schröederi synthesizes three bioactive fucoidans, the most abundant of which is fucan A. This fucoidan was extracted and its identity was confirmed by chemical analysis, Fourier-transform infrared spectroscopy (FTIR), and agarose gel electrophoresis. Thereafter, silver nanoparticles containing fucan A (AgFuc) were produced using an environmentally friendly synthesis method. AgFuc synthesis was analyzed via UV–vis spectroscopy and FTIR, which confirmed the presence of both silver and fucan A in the AgFuc product. Dynamic light scattering (DLS), X-ray diffraction, scanning electron microscopy, and atomic force microscopy revealed that the AgFuc particles were ~180.0 nm in size and spherical in shape. DLS further demonstrated that AgFuc was stable for five months. Coupled plasma optical emission spectrometry showed that the AgFuc particles contained 5% silver and 95% sugar. AgFuc was shown to be more effective in inhibiting the ability of parasites to reduce MTT than fucan A or silver, regardless of treatment time. In addition, AgFuc induced the death of ~60% of parasites by necrosis and ~17% by apoptosis. Therefore, AgFuc induces damage to the parasites’ mitochondria, which suggests that it is an anti-Trypanosoma cruzi agent. This is the first study to analyze silver nanoparticles containing fucan as an anti-Trypanosoma cruzi agent. Our data indicate that AgFuc nanoparticles have potential therapeutic applications, which should be determined via preclinical in vitro and in vivo studies. MDPI 2022-06-15 /pmc/articles/PMC9231105/ /pubmed/35745396 http://dx.doi.org/10.3390/nano12122059 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Souza, Adriana Oliveira
Oliveira, Johny Wysllas de Freitas
Moreno, Claudia Jéssica Gonsalves
de Medeiros, Mayra Jane Campos
Fernandes-Negreiros, Marília Medeiros
Souza, Flavia Roberta Monteiro
Pontes, Daniel Lima
Silva, Marcelo Sousa
Rocha, Hugo Alexandre Oliveira
Silver Nanoparticles Containing Fucoidan Synthesized by Green Method Have Anti-Trypanosoma cruzi Activity
title Silver Nanoparticles Containing Fucoidan Synthesized by Green Method Have Anti-Trypanosoma cruzi Activity
title_full Silver Nanoparticles Containing Fucoidan Synthesized by Green Method Have Anti-Trypanosoma cruzi Activity
title_fullStr Silver Nanoparticles Containing Fucoidan Synthesized by Green Method Have Anti-Trypanosoma cruzi Activity
title_full_unstemmed Silver Nanoparticles Containing Fucoidan Synthesized by Green Method Have Anti-Trypanosoma cruzi Activity
title_short Silver Nanoparticles Containing Fucoidan Synthesized by Green Method Have Anti-Trypanosoma cruzi Activity
title_sort silver nanoparticles containing fucoidan synthesized by green method have anti-trypanosoma cruzi activity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9231105/
https://www.ncbi.nlm.nih.gov/pubmed/35745396
http://dx.doi.org/10.3390/nano12122059
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