Cargando…
Toxicity Assessment of Resveratrol Liposomes-in-Hydrogel Delivery System by EpiVaginal(TM) Tissue Model
The natural polyphenol resveratrol (RES) has shown great potential as an antimicrobial, including against microbes associated with vaginal infections. To fully exploit the activities of RES, an all-natural ingredients formulation for RES delivery at vaginal site has been developed, namely liposomes...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9231258/ https://www.ncbi.nlm.nih.gov/pubmed/35745867 http://dx.doi.org/10.3390/pharmaceutics14061295 |
_version_ | 1784735288579325952 |
---|---|
author | Jøraholmen, May Wenche Damdimopoulou, Pauliina Acharya, Ganesh Škalko-Basnet, Nataša |
author_facet | Jøraholmen, May Wenche Damdimopoulou, Pauliina Acharya, Ganesh Škalko-Basnet, Nataša |
author_sort | Jøraholmen, May Wenche |
collection | PubMed |
description | The natural polyphenol resveratrol (RES) has shown great potential as an antimicrobial, including against microbes associated with vaginal infections. To fully exploit the activities of RES, an all-natural ingredients formulation for RES delivery at vaginal site has been developed, namely liposomes loaded with RES, incorporated into a chitosan hydrogel as secondary vehicle. Although considered non-toxic and safe on their own, the compatibility of the final formulation must be evaluated for its biocompatibility and non-irritancy to the vaginal mucosa. As a preclinical safety assessment, the impact of RES formulation on the tissue viability, the effect on barrier function and cell monolayer integrity, and cytotoxicity were evaluated using the cell-based vaginal tissue model, the EpiVaginal™ tissue. RES liposomes-in-hydrogel formulations neither affected the mitochondrial activity, nor the integrity of the cell monolayer in RES concentration up to 60 µg/mL. Moreover, the barrier function was maintained to a greater extent by RES in formulation, emphasizing the benefits of the delivery system. Additionally, none of the tested formulations expressed an increase in lactate dehydrogenase activity compared to the non-treated tissues. The evaluation of the RES delivery system suggests that it is non-irritant and biocompatible with vaginal tissue in vitro in the RES concentrations considered as therapeutic. |
format | Online Article Text |
id | pubmed-9231258 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-92312582022-06-25 Toxicity Assessment of Resveratrol Liposomes-in-Hydrogel Delivery System by EpiVaginal(TM) Tissue Model Jøraholmen, May Wenche Damdimopoulou, Pauliina Acharya, Ganesh Škalko-Basnet, Nataša Pharmaceutics Article The natural polyphenol resveratrol (RES) has shown great potential as an antimicrobial, including against microbes associated with vaginal infections. To fully exploit the activities of RES, an all-natural ingredients formulation for RES delivery at vaginal site has been developed, namely liposomes loaded with RES, incorporated into a chitosan hydrogel as secondary vehicle. Although considered non-toxic and safe on their own, the compatibility of the final formulation must be evaluated for its biocompatibility and non-irritancy to the vaginal mucosa. As a preclinical safety assessment, the impact of RES formulation on the tissue viability, the effect on barrier function and cell monolayer integrity, and cytotoxicity were evaluated using the cell-based vaginal tissue model, the EpiVaginal™ tissue. RES liposomes-in-hydrogel formulations neither affected the mitochondrial activity, nor the integrity of the cell monolayer in RES concentration up to 60 µg/mL. Moreover, the barrier function was maintained to a greater extent by RES in formulation, emphasizing the benefits of the delivery system. Additionally, none of the tested formulations expressed an increase in lactate dehydrogenase activity compared to the non-treated tissues. The evaluation of the RES delivery system suggests that it is non-irritant and biocompatible with vaginal tissue in vitro in the RES concentrations considered as therapeutic. MDPI 2022-06-17 /pmc/articles/PMC9231258/ /pubmed/35745867 http://dx.doi.org/10.3390/pharmaceutics14061295 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Jøraholmen, May Wenche Damdimopoulou, Pauliina Acharya, Ganesh Škalko-Basnet, Nataša Toxicity Assessment of Resveratrol Liposomes-in-Hydrogel Delivery System by EpiVaginal(TM) Tissue Model |
title | Toxicity Assessment of Resveratrol Liposomes-in-Hydrogel Delivery System by EpiVaginal(TM) Tissue Model |
title_full | Toxicity Assessment of Resveratrol Liposomes-in-Hydrogel Delivery System by EpiVaginal(TM) Tissue Model |
title_fullStr | Toxicity Assessment of Resveratrol Liposomes-in-Hydrogel Delivery System by EpiVaginal(TM) Tissue Model |
title_full_unstemmed | Toxicity Assessment of Resveratrol Liposomes-in-Hydrogel Delivery System by EpiVaginal(TM) Tissue Model |
title_short | Toxicity Assessment of Resveratrol Liposomes-in-Hydrogel Delivery System by EpiVaginal(TM) Tissue Model |
title_sort | toxicity assessment of resveratrol liposomes-in-hydrogel delivery system by epivaginal(tm) tissue model |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9231258/ https://www.ncbi.nlm.nih.gov/pubmed/35745867 http://dx.doi.org/10.3390/pharmaceutics14061295 |
work_keys_str_mv | AT jøraholmenmaywenche toxicityassessmentofresveratrolliposomesinhydrogeldeliverysystembyepivaginaltmtissuemodel AT damdimopouloupauliina toxicityassessmentofresveratrolliposomesinhydrogeldeliverysystembyepivaginaltmtissuemodel AT acharyaganesh toxicityassessmentofresveratrolliposomesinhydrogeldeliverysystembyepivaginaltmtissuemodel AT skalkobasnetnatasa toxicityassessmentofresveratrolliposomesinhydrogeldeliverysystembyepivaginaltmtissuemodel |