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Indoxyl Sulfate Induces Oxidative Changes in Plasma and Hemolysate

The deteriorating function of the kidneys in chronic kidney disease (CKD) is associated, among other things, with the retention of many unnecessary metabolic products in the body. Indoxyl sulfate (IS) belongs to the group of uremic toxins with a high protein binding affinity. Moreover, this compound...

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Autores principales: Pieniazek, Anna, Kopera, Michal, Gwozdzinski, Lukasz, Gwozdzinski, Krzysztof
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9231308/
https://www.ncbi.nlm.nih.gov/pubmed/35744968
http://dx.doi.org/10.3390/molecules27123848
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author Pieniazek, Anna
Kopera, Michal
Gwozdzinski, Lukasz
Gwozdzinski, Krzysztof
author_facet Pieniazek, Anna
Kopera, Michal
Gwozdzinski, Lukasz
Gwozdzinski, Krzysztof
author_sort Pieniazek, Anna
collection PubMed
description The deteriorating function of the kidneys in chronic kidney disease (CKD) is associated, among other things, with the retention of many unnecessary metabolic products in the body. Indoxyl sulfate (IS) belongs to the group of uremic toxins with a high protein binding affinity. Moreover, this compound can generate oxidative stress. We hypothesized that a high concentration of IS might induce oxidative changes in erythrocytes and plasma components, and could therefore contribute to CKD progression. In this study, we evaluated the influence of IS on the oxidative stress parameters in plasma and hemolysate. Moreover, as a result of the action of IS, we observed a decrease in the total antioxidant capacity and a change in the activity of catalase and superoxide dismutase in hemolysate and plasma. The obtained results indicate that IS induces oxidative damage to hemolysate and plasma components. Greater changes in the parameters of oxidative stress were observed in hemolysate than in plasma treated with indoxyl sulfate. The obtained results suggest that the increased concentration of IS in patients with chronic kidney disease may lead to a decrease in the lifespan of erythrocytes in their bloodstream.
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spelling pubmed-92313082022-06-25 Indoxyl Sulfate Induces Oxidative Changes in Plasma and Hemolysate Pieniazek, Anna Kopera, Michal Gwozdzinski, Lukasz Gwozdzinski, Krzysztof Molecules Article The deteriorating function of the kidneys in chronic kidney disease (CKD) is associated, among other things, with the retention of many unnecessary metabolic products in the body. Indoxyl sulfate (IS) belongs to the group of uremic toxins with a high protein binding affinity. Moreover, this compound can generate oxidative stress. We hypothesized that a high concentration of IS might induce oxidative changes in erythrocytes and plasma components, and could therefore contribute to CKD progression. In this study, we evaluated the influence of IS on the oxidative stress parameters in plasma and hemolysate. Moreover, as a result of the action of IS, we observed a decrease in the total antioxidant capacity and a change in the activity of catalase and superoxide dismutase in hemolysate and plasma. The obtained results indicate that IS induces oxidative damage to hemolysate and plasma components. Greater changes in the parameters of oxidative stress were observed in hemolysate than in plasma treated with indoxyl sulfate. The obtained results suggest that the increased concentration of IS in patients with chronic kidney disease may lead to a decrease in the lifespan of erythrocytes in their bloodstream. MDPI 2022-06-15 /pmc/articles/PMC9231308/ /pubmed/35744968 http://dx.doi.org/10.3390/molecules27123848 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pieniazek, Anna
Kopera, Michal
Gwozdzinski, Lukasz
Gwozdzinski, Krzysztof
Indoxyl Sulfate Induces Oxidative Changes in Plasma and Hemolysate
title Indoxyl Sulfate Induces Oxidative Changes in Plasma and Hemolysate
title_full Indoxyl Sulfate Induces Oxidative Changes in Plasma and Hemolysate
title_fullStr Indoxyl Sulfate Induces Oxidative Changes in Plasma and Hemolysate
title_full_unstemmed Indoxyl Sulfate Induces Oxidative Changes in Plasma and Hemolysate
title_short Indoxyl Sulfate Induces Oxidative Changes in Plasma and Hemolysate
title_sort indoxyl sulfate induces oxidative changes in plasma and hemolysate
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9231308/
https://www.ncbi.nlm.nih.gov/pubmed/35744968
http://dx.doi.org/10.3390/molecules27123848
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