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Effects of soft rock on soil properties and bacterial community in Mu Us Sandy Land, China

Soft rock is a new material that could be used for the improvement of Mu Us Sandy Land, China. It can be utilized for wind prevention and sand fixation, both of which are of great importance to ecological restoration aims and cultivated land replenishment in desert areas. Four treatments with differ...

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Autores principales: Guo, Zhen, Hui, Wei, Li, Juan, Yang, Chenxi, Zhang, Haiou, Wang, Huanyuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9231347/
https://www.ncbi.nlm.nih.gov/pubmed/35757169
http://dx.doi.org/10.7717/peerj.13561
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author Guo, Zhen
Hui, Wei
Li, Juan
Yang, Chenxi
Zhang, Haiou
Wang, Huanyuan
author_facet Guo, Zhen
Hui, Wei
Li, Juan
Yang, Chenxi
Zhang, Haiou
Wang, Huanyuan
author_sort Guo, Zhen
collection PubMed
description Soft rock is a new material that could be used for the improvement of Mu Us Sandy Land, China. It can be utilized for wind prevention and sand fixation, both of which are of great importance to ecological restoration aims and cultivated land replenishment in desert areas. Four treatments with different compound ratios of soft rock and sand—0:1 (CK), 1:5 (P1), 1:2 (P2), and 1:1 (P3)—were studied. Fluorescence quantitative PCR (qPCR) and high-throughput sequencing were used to analyze the structure and diversity of the bacterial community in the compound soil and its relationship with physical and chemical parameters in the soil. The results showed that in comparison to CK treatment, soil organic carbon (SOC), total nitrogen (TN), and NH(4)(+)-N increased significantly in the P1 treatment; available phosphorus (AP), available potassium (AK), and NO(3)(−)-N increased significantly in the P3 treatment. The bacterial gene copy number with P3 treatment was highest, showing a significant increase of 182.05% compared with the CK treatment. The three bacterial groups with the highest relative abundance at the phylum level were Actinobacteria, Proteobacteria, and Chloroflexi, accounting for more than 70% of the total population. The bacterial α diversity showed the same trend, the diversity and abundance indices of the P1 and P3 treatments were the highest, and the β diversity showed that the community structure of the two groups in these treatments were similar. norank_f__Roseiflexaceae and Gaiella (belonging to Actinobacteria) significantly differed with differing compound ratios in each treatment. NO(3)(−)-N, NH(4)(+)-N and SOC were the main factors affecting bacterial community structure, and had a significant positive correlation with Gaiella abundance. These species are known to play an important role in stabilizing the soil structure of sandy land. Overall, 1:5 and 1:1 compound soil mixtures were beneficial towards the microbial community of sandy land, which plays an important role in biological sand fixation. This study provides an important theoretical basis for the supplementation of arable land area and the improvement of sandy land productivity, and has a good promotion prospect.
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spelling pubmed-92313472022-06-25 Effects of soft rock on soil properties and bacterial community in Mu Us Sandy Land, China Guo, Zhen Hui, Wei Li, Juan Yang, Chenxi Zhang, Haiou Wang, Huanyuan PeerJ Agricultural Science Soft rock is a new material that could be used for the improvement of Mu Us Sandy Land, China. It can be utilized for wind prevention and sand fixation, both of which are of great importance to ecological restoration aims and cultivated land replenishment in desert areas. Four treatments with different compound ratios of soft rock and sand—0:1 (CK), 1:5 (P1), 1:2 (P2), and 1:1 (P3)—were studied. Fluorescence quantitative PCR (qPCR) and high-throughput sequencing were used to analyze the structure and diversity of the bacterial community in the compound soil and its relationship with physical and chemical parameters in the soil. The results showed that in comparison to CK treatment, soil organic carbon (SOC), total nitrogen (TN), and NH(4)(+)-N increased significantly in the P1 treatment; available phosphorus (AP), available potassium (AK), and NO(3)(−)-N increased significantly in the P3 treatment. The bacterial gene copy number with P3 treatment was highest, showing a significant increase of 182.05% compared with the CK treatment. The three bacterial groups with the highest relative abundance at the phylum level were Actinobacteria, Proteobacteria, and Chloroflexi, accounting for more than 70% of the total population. The bacterial α diversity showed the same trend, the diversity and abundance indices of the P1 and P3 treatments were the highest, and the β diversity showed that the community structure of the two groups in these treatments were similar. norank_f__Roseiflexaceae and Gaiella (belonging to Actinobacteria) significantly differed with differing compound ratios in each treatment. NO(3)(−)-N, NH(4)(+)-N and SOC were the main factors affecting bacterial community structure, and had a significant positive correlation with Gaiella abundance. These species are known to play an important role in stabilizing the soil structure of sandy land. Overall, 1:5 and 1:1 compound soil mixtures were beneficial towards the microbial community of sandy land, which plays an important role in biological sand fixation. This study provides an important theoretical basis for the supplementation of arable land area and the improvement of sandy land productivity, and has a good promotion prospect. PeerJ Inc. 2022-06-21 /pmc/articles/PMC9231347/ /pubmed/35757169 http://dx.doi.org/10.7717/peerj.13561 Text en © 2022 Guo et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Agricultural Science
Guo, Zhen
Hui, Wei
Li, Juan
Yang, Chenxi
Zhang, Haiou
Wang, Huanyuan
Effects of soft rock on soil properties and bacterial community in Mu Us Sandy Land, China
title Effects of soft rock on soil properties and bacterial community in Mu Us Sandy Land, China
title_full Effects of soft rock on soil properties and bacterial community in Mu Us Sandy Land, China
title_fullStr Effects of soft rock on soil properties and bacterial community in Mu Us Sandy Land, China
title_full_unstemmed Effects of soft rock on soil properties and bacterial community in Mu Us Sandy Land, China
title_short Effects of soft rock on soil properties and bacterial community in Mu Us Sandy Land, China
title_sort effects of soft rock on soil properties and bacterial community in mu us sandy land, china
topic Agricultural Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9231347/
https://www.ncbi.nlm.nih.gov/pubmed/35757169
http://dx.doi.org/10.7717/peerj.13561
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