Cargando…

Diagnostic Accuracy of LDBIO-Toxo II IgG and IgM Western Blot in Suspected Seroconversion in Pregnancy: A Multicentre Study

The high sensitivity of the automated tests used for Toxoplasma gondii serology can yield false-positive IgM results due to aspecific reactions. On the other hand, specific therapy can delay IgG production and, therefore, the diagnosis of seroconversion. There is a need for confirmation tests to ear...

Descripción completa

Detalles Bibliográficos
Autores principales: Meroni, Valeria, Genco, Francesca, Scudeller, Luigia, Brenier-Pinchart, Marie-Pierre, Fricker-Hidalgo, Hélène, L’Ollivier, Coralie, Paris, Luc, Pelloux, Hervé
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9231380/
https://www.ncbi.nlm.nih.gov/pubmed/35745519
http://dx.doi.org/10.3390/pathogens11060665
_version_ 1784735324816015360
author Meroni, Valeria
Genco, Francesca
Scudeller, Luigia
Brenier-Pinchart, Marie-Pierre
Fricker-Hidalgo, Hélène
L’Ollivier, Coralie
Paris, Luc
Pelloux, Hervé
author_facet Meroni, Valeria
Genco, Francesca
Scudeller, Luigia
Brenier-Pinchart, Marie-Pierre
Fricker-Hidalgo, Hélène
L’Ollivier, Coralie
Paris, Luc
Pelloux, Hervé
author_sort Meroni, Valeria
collection PubMed
description The high sensitivity of the automated tests used for Toxoplasma gondii serology can yield false-positive IgM results due to aspecific reactions. On the other hand, specific therapy can delay IgG production and, therefore, the diagnosis of seroconversion. There is a need for confirmation tests to early detect seroconversions during pregnancy. We conducted a multicentre study to evaluate the diagnostic accuracy of the Toxo II IgG and a new, not yet commercialised Toxo II IgM western blot (WB) (LDBio diagnostics Lyon France) on 229 sera corresponding to 93 patients with seroconversions and 158 sera corresponding to 68 patients with nonspecific IgM. Sensitivity was 97.8% for IgM WB and 98.9% for IgG WB. Specificity was 89.7% and 100%, respectively. The concordance between IgM and IgG Toxo WB with the final diagnosis was very good, K = 0.89 and K = 0.99, respectively. In 5 cases (5.4%), the appearance of IgM, and in 55 cases (59.1%), the appearance of IgG was recorded by WB earlier than by traditional tests. In 10 cases (10.8%), IgM was detected after the traditional tests and in 2 cases (2.2%) for IgG. The association of IgG and IgM WB on the same sample not only detected all seroconversions but also correctly identified most of the false-positive results.
format Online
Article
Text
id pubmed-9231380
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-92313802022-06-25 Diagnostic Accuracy of LDBIO-Toxo II IgG and IgM Western Blot in Suspected Seroconversion in Pregnancy: A Multicentre Study Meroni, Valeria Genco, Francesca Scudeller, Luigia Brenier-Pinchart, Marie-Pierre Fricker-Hidalgo, Hélène L’Ollivier, Coralie Paris, Luc Pelloux, Hervé Pathogens Article The high sensitivity of the automated tests used for Toxoplasma gondii serology can yield false-positive IgM results due to aspecific reactions. On the other hand, specific therapy can delay IgG production and, therefore, the diagnosis of seroconversion. There is a need for confirmation tests to early detect seroconversions during pregnancy. We conducted a multicentre study to evaluate the diagnostic accuracy of the Toxo II IgG and a new, not yet commercialised Toxo II IgM western blot (WB) (LDBio diagnostics Lyon France) on 229 sera corresponding to 93 patients with seroconversions and 158 sera corresponding to 68 patients with nonspecific IgM. Sensitivity was 97.8% for IgM WB and 98.9% for IgG WB. Specificity was 89.7% and 100%, respectively. The concordance between IgM and IgG Toxo WB with the final diagnosis was very good, K = 0.89 and K = 0.99, respectively. In 5 cases (5.4%), the appearance of IgM, and in 55 cases (59.1%), the appearance of IgG was recorded by WB earlier than by traditional tests. In 10 cases (10.8%), IgM was detected after the traditional tests and in 2 cases (2.2%) for IgG. The association of IgG and IgM WB on the same sample not only detected all seroconversions but also correctly identified most of the false-positive results. MDPI 2022-06-08 /pmc/articles/PMC9231380/ /pubmed/35745519 http://dx.doi.org/10.3390/pathogens11060665 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Meroni, Valeria
Genco, Francesca
Scudeller, Luigia
Brenier-Pinchart, Marie-Pierre
Fricker-Hidalgo, Hélène
L’Ollivier, Coralie
Paris, Luc
Pelloux, Hervé
Diagnostic Accuracy of LDBIO-Toxo II IgG and IgM Western Blot in Suspected Seroconversion in Pregnancy: A Multicentre Study
title Diagnostic Accuracy of LDBIO-Toxo II IgG and IgM Western Blot in Suspected Seroconversion in Pregnancy: A Multicentre Study
title_full Diagnostic Accuracy of LDBIO-Toxo II IgG and IgM Western Blot in Suspected Seroconversion in Pregnancy: A Multicentre Study
title_fullStr Diagnostic Accuracy of LDBIO-Toxo II IgG and IgM Western Blot in Suspected Seroconversion in Pregnancy: A Multicentre Study
title_full_unstemmed Diagnostic Accuracy of LDBIO-Toxo II IgG and IgM Western Blot in Suspected Seroconversion in Pregnancy: A Multicentre Study
title_short Diagnostic Accuracy of LDBIO-Toxo II IgG and IgM Western Blot in Suspected Seroconversion in Pregnancy: A Multicentre Study
title_sort diagnostic accuracy of ldbio-toxo ii igg and igm western blot in suspected seroconversion in pregnancy: a multicentre study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9231380/
https://www.ncbi.nlm.nih.gov/pubmed/35745519
http://dx.doi.org/10.3390/pathogens11060665
work_keys_str_mv AT meronivaleria diagnosticaccuracyofldbiotoxoiiiggandigmwesternblotinsuspectedseroconversioninpregnancyamulticentrestudy
AT gencofrancesca diagnosticaccuracyofldbiotoxoiiiggandigmwesternblotinsuspectedseroconversioninpregnancyamulticentrestudy
AT scudellerluigia diagnosticaccuracyofldbiotoxoiiiggandigmwesternblotinsuspectedseroconversioninpregnancyamulticentrestudy
AT brenierpinchartmariepierre diagnosticaccuracyofldbiotoxoiiiggandigmwesternblotinsuspectedseroconversioninpregnancyamulticentrestudy
AT frickerhidalgohelene diagnosticaccuracyofldbiotoxoiiiggandigmwesternblotinsuspectedseroconversioninpregnancyamulticentrestudy
AT lolliviercoralie diagnosticaccuracyofldbiotoxoiiiggandigmwesternblotinsuspectedseroconversioninpregnancyamulticentrestudy
AT parisluc diagnosticaccuracyofldbiotoxoiiiggandigmwesternblotinsuspectedseroconversioninpregnancyamulticentrestudy
AT pellouxherve diagnosticaccuracyofldbiotoxoiiiggandigmwesternblotinsuspectedseroconversioninpregnancyamulticentrestudy