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Determination of quality markers for quality control of Zanthoxylum nitidum using ultra-performance liquid chromatography coupled with near infrared spectroscopy

With the increasing demand for quality control in the traditional Chinese medicine industry, there is a need for the development of quality markers and a quick, non-destructive technique for the discrimination of related species. In our previous study, ultra-performance liquid chromatography (UPLC)...

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Autores principales: Wang, Xinhong, Wu, Qingwen, Li, Lulu, Wang, Peng, Wang, Yue, Wei, Weifeng, Ma, Xiaojun, Shu, Jing, Zhang, Kai, Ma, Dongming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9231700/
https://www.ncbi.nlm.nih.gov/pubmed/35749476
http://dx.doi.org/10.1371/journal.pone.0270315
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author Wang, Xinhong
Wu, Qingwen
Li, Lulu
Wang, Peng
Wang, Yue
Wei, Weifeng
Ma, Xiaojun
Shu, Jing
Zhang, Kai
Ma, Dongming
author_facet Wang, Xinhong
Wu, Qingwen
Li, Lulu
Wang, Peng
Wang, Yue
Wei, Weifeng
Ma, Xiaojun
Shu, Jing
Zhang, Kai
Ma, Dongming
author_sort Wang, Xinhong
collection PubMed
description With the increasing demand for quality control in the traditional Chinese medicine industry, there is a need for the development of quality markers and a quick, non-destructive technique for the discrimination of related species. In our previous study, ultra-performance liquid chromatography (UPLC) was used for the simultaneous determination of five compounds, including three alkaloids (nitidine chloride, chelerythrine, and magnoflorine), one flavonoid (aurantiamarin), and one lignan (sesamin). In this study, the simultaneous quantification of the above-mentioned compounds could be used to discriminate the powders of roots from those of stems. To further test the reliability of the five compounds, seventy-two batches of wild and seventy-five batches of cultivated Zanthoxylum nitidum samples collected from Guangdong, Guangxi, and Fujian provinces in China were analyzed by UPLC and near-infrared spectroscopy (NIRS). In general, the quantitative results of UPLC were consistent with those of NIRS, and cultivated Z. nitidum has similar major bioactive compounds as the wild one, as supported by principal component analysis. Consequently, these five major bioactive compounds are suggested as potential quality markers. In addition, the NIRS method with discriminant analysis successfully differentiated Z. nitidum from three related species (Z. avicennae, Z. scandens and Toddalia asiatica) of the Rutaceae family. In summary, this study provides a method for the rapid identification of Z. nitidum and discrimination of root and stem powders, and suggests five compounds as quality markers for the evaluation of Z. nitidum.
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spelling pubmed-92317002022-06-25 Determination of quality markers for quality control of Zanthoxylum nitidum using ultra-performance liquid chromatography coupled with near infrared spectroscopy Wang, Xinhong Wu, Qingwen Li, Lulu Wang, Peng Wang, Yue Wei, Weifeng Ma, Xiaojun Shu, Jing Zhang, Kai Ma, Dongming PLoS One Research Article With the increasing demand for quality control in the traditional Chinese medicine industry, there is a need for the development of quality markers and a quick, non-destructive technique for the discrimination of related species. In our previous study, ultra-performance liquid chromatography (UPLC) was used for the simultaneous determination of five compounds, including three alkaloids (nitidine chloride, chelerythrine, and magnoflorine), one flavonoid (aurantiamarin), and one lignan (sesamin). In this study, the simultaneous quantification of the above-mentioned compounds could be used to discriminate the powders of roots from those of stems. To further test the reliability of the five compounds, seventy-two batches of wild and seventy-five batches of cultivated Zanthoxylum nitidum samples collected from Guangdong, Guangxi, and Fujian provinces in China were analyzed by UPLC and near-infrared spectroscopy (NIRS). In general, the quantitative results of UPLC were consistent with those of NIRS, and cultivated Z. nitidum has similar major bioactive compounds as the wild one, as supported by principal component analysis. Consequently, these five major bioactive compounds are suggested as potential quality markers. In addition, the NIRS method with discriminant analysis successfully differentiated Z. nitidum from three related species (Z. avicennae, Z. scandens and Toddalia asiatica) of the Rutaceae family. In summary, this study provides a method for the rapid identification of Z. nitidum and discrimination of root and stem powders, and suggests five compounds as quality markers for the evaluation of Z. nitidum. Public Library of Science 2022-06-24 /pmc/articles/PMC9231700/ /pubmed/35749476 http://dx.doi.org/10.1371/journal.pone.0270315 Text en © 2022 Wang et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Wang, Xinhong
Wu, Qingwen
Li, Lulu
Wang, Peng
Wang, Yue
Wei, Weifeng
Ma, Xiaojun
Shu, Jing
Zhang, Kai
Ma, Dongming
Determination of quality markers for quality control of Zanthoxylum nitidum using ultra-performance liquid chromatography coupled with near infrared spectroscopy
title Determination of quality markers for quality control of Zanthoxylum nitidum using ultra-performance liquid chromatography coupled with near infrared spectroscopy
title_full Determination of quality markers for quality control of Zanthoxylum nitidum using ultra-performance liquid chromatography coupled with near infrared spectroscopy
title_fullStr Determination of quality markers for quality control of Zanthoxylum nitidum using ultra-performance liquid chromatography coupled with near infrared spectroscopy
title_full_unstemmed Determination of quality markers for quality control of Zanthoxylum nitidum using ultra-performance liquid chromatography coupled with near infrared spectroscopy
title_short Determination of quality markers for quality control of Zanthoxylum nitidum using ultra-performance liquid chromatography coupled with near infrared spectroscopy
title_sort determination of quality markers for quality control of zanthoxylum nitidum using ultra-performance liquid chromatography coupled with near infrared spectroscopy
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9231700/
https://www.ncbi.nlm.nih.gov/pubmed/35749476
http://dx.doi.org/10.1371/journal.pone.0270315
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