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Eradication of avian leukosis virus subgroups J and K in broiler cross chickens by selection against infected birds using multilocus PCR
The avian leukosis virus (ALV) is a serious threat to sustainable and economically viable commercial poultry management world-wide. Active infections can result in more than 20% flock loss, resulting in significant economic damage. ALV detection and elimination from flocks and breeding programs is c...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9231750/ https://www.ncbi.nlm.nih.gov/pubmed/35749432 http://dx.doi.org/10.1371/journal.pone.0269525 |
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author | Borodin, Alexander M. Emanuilova, Zhanna V. Smolov, Sergei V. Ogneva, Olga A. Konovalova, Nina V. Terentyeva, Elena V. Serova, Natalia Y. Efimov, D. N. Fisinin, V. I. Greenberg, Anthony J. Alekseev, Yakov I. |
author_facet | Borodin, Alexander M. Emanuilova, Zhanna V. Smolov, Sergei V. Ogneva, Olga A. Konovalova, Nina V. Terentyeva, Elena V. Serova, Natalia Y. Efimov, D. N. Fisinin, V. I. Greenberg, Anthony J. Alekseev, Yakov I. |
author_sort | Borodin, Alexander M. |
collection | PubMed |
description | The avian leukosis virus (ALV) is a serious threat to sustainable and economically viable commercial poultry management world-wide. Active infections can result in more than 20% flock loss, resulting in significant economic damage. ALV detection and elimination from flocks and breeding programs is complicated by high sequence variability and the presence of endogenous virus copies which show up as false positives in assays. Previously-developed approaches to virus detection are either too labor-intensive to implement on an industrial scale or suffer from high false negative or positive rates. We developed a novel multi-locus multiplex quantitative real-time PCR system to detect viruses belonging to the J and K genetic subgroups that are particularly prevalent in our region. We used this system to eradicate ALV from our broiler breeding program comprising thousands of individuals. Our approach can be generalized to other ALV subgroups and other highly genetically diverse pathogens. |
format | Online Article Text |
id | pubmed-9231750 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-92317502022-06-25 Eradication of avian leukosis virus subgroups J and K in broiler cross chickens by selection against infected birds using multilocus PCR Borodin, Alexander M. Emanuilova, Zhanna V. Smolov, Sergei V. Ogneva, Olga A. Konovalova, Nina V. Terentyeva, Elena V. Serova, Natalia Y. Efimov, D. N. Fisinin, V. I. Greenberg, Anthony J. Alekseev, Yakov I. PLoS One Research Article The avian leukosis virus (ALV) is a serious threat to sustainable and economically viable commercial poultry management world-wide. Active infections can result in more than 20% flock loss, resulting in significant economic damage. ALV detection and elimination from flocks and breeding programs is complicated by high sequence variability and the presence of endogenous virus copies which show up as false positives in assays. Previously-developed approaches to virus detection are either too labor-intensive to implement on an industrial scale or suffer from high false negative or positive rates. We developed a novel multi-locus multiplex quantitative real-time PCR system to detect viruses belonging to the J and K genetic subgroups that are particularly prevalent in our region. We used this system to eradicate ALV from our broiler breeding program comprising thousands of individuals. Our approach can be generalized to other ALV subgroups and other highly genetically diverse pathogens. Public Library of Science 2022-06-24 /pmc/articles/PMC9231750/ /pubmed/35749432 http://dx.doi.org/10.1371/journal.pone.0269525 Text en © 2022 Borodin et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Borodin, Alexander M. Emanuilova, Zhanna V. Smolov, Sergei V. Ogneva, Olga A. Konovalova, Nina V. Terentyeva, Elena V. Serova, Natalia Y. Efimov, D. N. Fisinin, V. I. Greenberg, Anthony J. Alekseev, Yakov I. Eradication of avian leukosis virus subgroups J and K in broiler cross chickens by selection against infected birds using multilocus PCR |
title | Eradication of avian leukosis virus subgroups J and K in broiler cross chickens by selection against infected birds using multilocus PCR |
title_full | Eradication of avian leukosis virus subgroups J and K in broiler cross chickens by selection against infected birds using multilocus PCR |
title_fullStr | Eradication of avian leukosis virus subgroups J and K in broiler cross chickens by selection against infected birds using multilocus PCR |
title_full_unstemmed | Eradication of avian leukosis virus subgroups J and K in broiler cross chickens by selection against infected birds using multilocus PCR |
title_short | Eradication of avian leukosis virus subgroups J and K in broiler cross chickens by selection against infected birds using multilocus PCR |
title_sort | eradication of avian leukosis virus subgroups j and k in broiler cross chickens by selection against infected birds using multilocus pcr |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9231750/ https://www.ncbi.nlm.nih.gov/pubmed/35749432 http://dx.doi.org/10.1371/journal.pone.0269525 |
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