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Eradication of avian leukosis virus subgroups J and K in broiler cross chickens by selection against infected birds using multilocus PCR

The avian leukosis virus (ALV) is a serious threat to sustainable and economically viable commercial poultry management world-wide. Active infections can result in more than 20% flock loss, resulting in significant economic damage. ALV detection and elimination from flocks and breeding programs is c...

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Autores principales: Borodin, Alexander M., Emanuilova, Zhanna V., Smolov, Sergei V., Ogneva, Olga A., Konovalova, Nina V., Terentyeva, Elena V., Serova, Natalia Y., Efimov, D. N., Fisinin, V. I., Greenberg, Anthony J., Alekseev, Yakov I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9231750/
https://www.ncbi.nlm.nih.gov/pubmed/35749432
http://dx.doi.org/10.1371/journal.pone.0269525
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author Borodin, Alexander M.
Emanuilova, Zhanna V.
Smolov, Sergei V.
Ogneva, Olga A.
Konovalova, Nina V.
Terentyeva, Elena V.
Serova, Natalia Y.
Efimov, D. N.
Fisinin, V. I.
Greenberg, Anthony J.
Alekseev, Yakov I.
author_facet Borodin, Alexander M.
Emanuilova, Zhanna V.
Smolov, Sergei V.
Ogneva, Olga A.
Konovalova, Nina V.
Terentyeva, Elena V.
Serova, Natalia Y.
Efimov, D. N.
Fisinin, V. I.
Greenberg, Anthony J.
Alekseev, Yakov I.
author_sort Borodin, Alexander M.
collection PubMed
description The avian leukosis virus (ALV) is a serious threat to sustainable and economically viable commercial poultry management world-wide. Active infections can result in more than 20% flock loss, resulting in significant economic damage. ALV detection and elimination from flocks and breeding programs is complicated by high sequence variability and the presence of endogenous virus copies which show up as false positives in assays. Previously-developed approaches to virus detection are either too labor-intensive to implement on an industrial scale or suffer from high false negative or positive rates. We developed a novel multi-locus multiplex quantitative real-time PCR system to detect viruses belonging to the J and K genetic subgroups that are particularly prevalent in our region. We used this system to eradicate ALV from our broiler breeding program comprising thousands of individuals. Our approach can be generalized to other ALV subgroups and other highly genetically diverse pathogens.
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spelling pubmed-92317502022-06-25 Eradication of avian leukosis virus subgroups J and K in broiler cross chickens by selection against infected birds using multilocus PCR Borodin, Alexander M. Emanuilova, Zhanna V. Smolov, Sergei V. Ogneva, Olga A. Konovalova, Nina V. Terentyeva, Elena V. Serova, Natalia Y. Efimov, D. N. Fisinin, V. I. Greenberg, Anthony J. Alekseev, Yakov I. PLoS One Research Article The avian leukosis virus (ALV) is a serious threat to sustainable and economically viable commercial poultry management world-wide. Active infections can result in more than 20% flock loss, resulting in significant economic damage. ALV detection and elimination from flocks and breeding programs is complicated by high sequence variability and the presence of endogenous virus copies which show up as false positives in assays. Previously-developed approaches to virus detection are either too labor-intensive to implement on an industrial scale or suffer from high false negative or positive rates. We developed a novel multi-locus multiplex quantitative real-time PCR system to detect viruses belonging to the J and K genetic subgroups that are particularly prevalent in our region. We used this system to eradicate ALV from our broiler breeding program comprising thousands of individuals. Our approach can be generalized to other ALV subgroups and other highly genetically diverse pathogens. Public Library of Science 2022-06-24 /pmc/articles/PMC9231750/ /pubmed/35749432 http://dx.doi.org/10.1371/journal.pone.0269525 Text en © 2022 Borodin et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Borodin, Alexander M.
Emanuilova, Zhanna V.
Smolov, Sergei V.
Ogneva, Olga A.
Konovalova, Nina V.
Terentyeva, Elena V.
Serova, Natalia Y.
Efimov, D. N.
Fisinin, V. I.
Greenberg, Anthony J.
Alekseev, Yakov I.
Eradication of avian leukosis virus subgroups J and K in broiler cross chickens by selection against infected birds using multilocus PCR
title Eradication of avian leukosis virus subgroups J and K in broiler cross chickens by selection against infected birds using multilocus PCR
title_full Eradication of avian leukosis virus subgroups J and K in broiler cross chickens by selection against infected birds using multilocus PCR
title_fullStr Eradication of avian leukosis virus subgroups J and K in broiler cross chickens by selection against infected birds using multilocus PCR
title_full_unstemmed Eradication of avian leukosis virus subgroups J and K in broiler cross chickens by selection against infected birds using multilocus PCR
title_short Eradication of avian leukosis virus subgroups J and K in broiler cross chickens by selection against infected birds using multilocus PCR
title_sort eradication of avian leukosis virus subgroups j and k in broiler cross chickens by selection against infected birds using multilocus pcr
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9231750/
https://www.ncbi.nlm.nih.gov/pubmed/35749432
http://dx.doi.org/10.1371/journal.pone.0269525
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