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Genomic Analysis of a Highly Virulent NDM-1-Producing Escherichia coli ST162 Infecting a Pygmy Sperm Whale (Kogia breviceps) in South America

Carbapenemase-producing Enterobacterales are rapidly spreading and adapting to different environments beyond hospital settings. During COVID-19 lockdown, a carbapenem-resistant NDM-1-positive Escherichia coli isolate (BA01 strain) was recovered from a pygmy sperm whale (Kogia breviceps), which was f...

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Autores principales: Sellera, Fábio P., Cardoso, Brenda, Fuentes-Castillo, Danny, Esposito, Fernanda, Sano, Elder, Fontana, Herrison, Fuga, Bruna, Goldberg, Daphne W., Seabra, Lourdes A. V., Antonelli, Marzia, Sandri, Sandro, Kolesnikovas, Cristiane K. M., Lincopan, Nilton
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9231830/
https://www.ncbi.nlm.nih.gov/pubmed/35755998
http://dx.doi.org/10.3389/fmicb.2022.915375
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author Sellera, Fábio P.
Cardoso, Brenda
Fuentes-Castillo, Danny
Esposito, Fernanda
Sano, Elder
Fontana, Herrison
Fuga, Bruna
Goldberg, Daphne W.
Seabra, Lourdes A. V.
Antonelli, Marzia
Sandri, Sandro
Kolesnikovas, Cristiane K. M.
Lincopan, Nilton
author_facet Sellera, Fábio P.
Cardoso, Brenda
Fuentes-Castillo, Danny
Esposito, Fernanda
Sano, Elder
Fontana, Herrison
Fuga, Bruna
Goldberg, Daphne W.
Seabra, Lourdes A. V.
Antonelli, Marzia
Sandri, Sandro
Kolesnikovas, Cristiane K. M.
Lincopan, Nilton
author_sort Sellera, Fábio P.
collection PubMed
description Carbapenemase-producing Enterobacterales are rapidly spreading and adapting to different environments beyond hospital settings. During COVID-19 lockdown, a carbapenem-resistant NDM-1-positive Escherichia coli isolate (BA01 strain) was recovered from a pygmy sperm whale (Kogia breviceps), which was found stranded on the southern coast of Brazil. BA01 strain belonged to the global sequence type (ST) 162 and carried the bla(NDM–1), besides other medically important antimicrobial resistance genes. Additionally, genes associated with resistance to heavy metals, biocides, and glyphosate were also detected. Halophilic behavior (tolerance to > 10% NaCl) of BA01 strain was confirmed by tolerance tests of NaCl minimal inhibitory concentration, whereas halotolerance associated genes katE and nhaA, which encodes for catalase and Na(+)/H(+) antiporter cytoplasmic membrane, respectively, were in silico confirmed. Phylogenomics clustered BA01 with poultry- and human-associated ST162 lineages circulating in European and Asian countries. Important virulence genes, including the astA (a gene encoding an enterotoxin associated with human and animal infections) were detected, whereas in vivo experiments using the Galleria mellonella infection model confirmed the virulent behavior of the BA01 strain. WHO critical priority carbapenemase-producing pathogens in coastal water are an emerging threat that deserves the urgent need to assess the role of the aquatic environment in its global epidemiology.
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spelling pubmed-92318302022-06-25 Genomic Analysis of a Highly Virulent NDM-1-Producing Escherichia coli ST162 Infecting a Pygmy Sperm Whale (Kogia breviceps) in South America Sellera, Fábio P. Cardoso, Brenda Fuentes-Castillo, Danny Esposito, Fernanda Sano, Elder Fontana, Herrison Fuga, Bruna Goldberg, Daphne W. Seabra, Lourdes A. V. Antonelli, Marzia Sandri, Sandro Kolesnikovas, Cristiane K. M. Lincopan, Nilton Front Microbiol Microbiology Carbapenemase-producing Enterobacterales are rapidly spreading and adapting to different environments beyond hospital settings. During COVID-19 lockdown, a carbapenem-resistant NDM-1-positive Escherichia coli isolate (BA01 strain) was recovered from a pygmy sperm whale (Kogia breviceps), which was found stranded on the southern coast of Brazil. BA01 strain belonged to the global sequence type (ST) 162 and carried the bla(NDM–1), besides other medically important antimicrobial resistance genes. Additionally, genes associated with resistance to heavy metals, biocides, and glyphosate were also detected. Halophilic behavior (tolerance to > 10% NaCl) of BA01 strain was confirmed by tolerance tests of NaCl minimal inhibitory concentration, whereas halotolerance associated genes katE and nhaA, which encodes for catalase and Na(+)/H(+) antiporter cytoplasmic membrane, respectively, were in silico confirmed. Phylogenomics clustered BA01 with poultry- and human-associated ST162 lineages circulating in European and Asian countries. Important virulence genes, including the astA (a gene encoding an enterotoxin associated with human and animal infections) were detected, whereas in vivo experiments using the Galleria mellonella infection model confirmed the virulent behavior of the BA01 strain. WHO critical priority carbapenemase-producing pathogens in coastal water are an emerging threat that deserves the urgent need to assess the role of the aquatic environment in its global epidemiology. Frontiers Media S.A. 2022-06-10 /pmc/articles/PMC9231830/ /pubmed/35755998 http://dx.doi.org/10.3389/fmicb.2022.915375 Text en Copyright © 2022 Sellera, Cardoso, Fuentes-Castillo, Esposito, Sano, Fontana, Fuga, Goldberg, Seabra, Antonelli, Sandri, Kolesnikovas and Lincopan. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Sellera, Fábio P.
Cardoso, Brenda
Fuentes-Castillo, Danny
Esposito, Fernanda
Sano, Elder
Fontana, Herrison
Fuga, Bruna
Goldberg, Daphne W.
Seabra, Lourdes A. V.
Antonelli, Marzia
Sandri, Sandro
Kolesnikovas, Cristiane K. M.
Lincopan, Nilton
Genomic Analysis of a Highly Virulent NDM-1-Producing Escherichia coli ST162 Infecting a Pygmy Sperm Whale (Kogia breviceps) in South America
title Genomic Analysis of a Highly Virulent NDM-1-Producing Escherichia coli ST162 Infecting a Pygmy Sperm Whale (Kogia breviceps) in South America
title_full Genomic Analysis of a Highly Virulent NDM-1-Producing Escherichia coli ST162 Infecting a Pygmy Sperm Whale (Kogia breviceps) in South America
title_fullStr Genomic Analysis of a Highly Virulent NDM-1-Producing Escherichia coli ST162 Infecting a Pygmy Sperm Whale (Kogia breviceps) in South America
title_full_unstemmed Genomic Analysis of a Highly Virulent NDM-1-Producing Escherichia coli ST162 Infecting a Pygmy Sperm Whale (Kogia breviceps) in South America
title_short Genomic Analysis of a Highly Virulent NDM-1-Producing Escherichia coli ST162 Infecting a Pygmy Sperm Whale (Kogia breviceps) in South America
title_sort genomic analysis of a highly virulent ndm-1-producing escherichia coli st162 infecting a pygmy sperm whale (kogia breviceps) in south america
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9231830/
https://www.ncbi.nlm.nih.gov/pubmed/35755998
http://dx.doi.org/10.3389/fmicb.2022.915375
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