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Oscillatory movement of a dynein-microtubule complex crosslinked with DNA origami
Bending of cilia and flagella occurs when axonemal dynein molecules on one side of the axoneme produce force and move toward the microtubule (MT) minus end. These dyneins are then pulled back when the axoneme bends in the other direction, meaning oscillatory back and forth movement of dynein during...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9232216/ https://www.ncbi.nlm.nih.gov/pubmed/35749159 http://dx.doi.org/10.7554/eLife.76357 |
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author | Abdellatef, Shimaa A Tadakuma, Hisashi Yan, Kangmin Fujiwara, Takashi Fukumoto, Kodai Kondo, Yuichi Takazaki, Hiroko Boudria, Rofia Yasunaga, Takuo Higuchi, Hideo Hirose, Keiko |
author_facet | Abdellatef, Shimaa A Tadakuma, Hisashi Yan, Kangmin Fujiwara, Takashi Fukumoto, Kodai Kondo, Yuichi Takazaki, Hiroko Boudria, Rofia Yasunaga, Takuo Higuchi, Hideo Hirose, Keiko |
author_sort | Abdellatef, Shimaa A |
collection | PubMed |
description | Bending of cilia and flagella occurs when axonemal dynein molecules on one side of the axoneme produce force and move toward the microtubule (MT) minus end. These dyneins are then pulled back when the axoneme bends in the other direction, meaning oscillatory back and forth movement of dynein during repetitive bending of cilia/flagella. There are various factors that may regulate the dynein activity, e.g. the nexin-dynein regulatory complex, radial spokes, and central apparatus. In order to understand the basic mechanism of dynein’s oscillatory movement, we constructed a simple model system composed of MTs, outer-arm dyneins, and crosslinks between the MTs made of DNA origami. Electron microscopy (EM) showed pairs of parallel MTs crossbridged by patches of regularly arranged dynein molecules bound in two different orientations, depending on which of the MTs their tails bind to. The oppositely oriented dyneins are expected to produce opposing forces when the pair of MTs have the same polarity. Optical trapping experiments showed that the dynein-MT-DNA-origami complex actually oscillates back and forth after photolysis of caged ATP. Intriguingly, the complex, when held at one end, showed repetitive bending motions. The results show that a simple system composed of ensembles of oppositely oriented dyneins, MTs, and inter-MT crosslinkers, without any additional regulatory structures, has an intrinsic ability to cause oscillation and repetitive bending motions. |
format | Online Article Text |
id | pubmed-9232216 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-92322162022-06-25 Oscillatory movement of a dynein-microtubule complex crosslinked with DNA origami Abdellatef, Shimaa A Tadakuma, Hisashi Yan, Kangmin Fujiwara, Takashi Fukumoto, Kodai Kondo, Yuichi Takazaki, Hiroko Boudria, Rofia Yasunaga, Takuo Higuchi, Hideo Hirose, Keiko eLife Cell Biology Bending of cilia and flagella occurs when axonemal dynein molecules on one side of the axoneme produce force and move toward the microtubule (MT) minus end. These dyneins are then pulled back when the axoneme bends in the other direction, meaning oscillatory back and forth movement of dynein during repetitive bending of cilia/flagella. There are various factors that may regulate the dynein activity, e.g. the nexin-dynein regulatory complex, radial spokes, and central apparatus. In order to understand the basic mechanism of dynein’s oscillatory movement, we constructed a simple model system composed of MTs, outer-arm dyneins, and crosslinks between the MTs made of DNA origami. Electron microscopy (EM) showed pairs of parallel MTs crossbridged by patches of regularly arranged dynein molecules bound in two different orientations, depending on which of the MTs their tails bind to. The oppositely oriented dyneins are expected to produce opposing forces when the pair of MTs have the same polarity. Optical trapping experiments showed that the dynein-MT-DNA-origami complex actually oscillates back and forth after photolysis of caged ATP. Intriguingly, the complex, when held at one end, showed repetitive bending motions. The results show that a simple system composed of ensembles of oppositely oriented dyneins, MTs, and inter-MT crosslinkers, without any additional regulatory structures, has an intrinsic ability to cause oscillation and repetitive bending motions. eLife Sciences Publications, Ltd 2022-06-24 /pmc/articles/PMC9232216/ /pubmed/35749159 http://dx.doi.org/10.7554/eLife.76357 Text en © 2022, Abdellatef et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Cell Biology Abdellatef, Shimaa A Tadakuma, Hisashi Yan, Kangmin Fujiwara, Takashi Fukumoto, Kodai Kondo, Yuichi Takazaki, Hiroko Boudria, Rofia Yasunaga, Takuo Higuchi, Hideo Hirose, Keiko Oscillatory movement of a dynein-microtubule complex crosslinked with DNA origami |
title | Oscillatory movement of a dynein-microtubule complex crosslinked with DNA origami |
title_full | Oscillatory movement of a dynein-microtubule complex crosslinked with DNA origami |
title_fullStr | Oscillatory movement of a dynein-microtubule complex crosslinked with DNA origami |
title_full_unstemmed | Oscillatory movement of a dynein-microtubule complex crosslinked with DNA origami |
title_short | Oscillatory movement of a dynein-microtubule complex crosslinked with DNA origami |
title_sort | oscillatory movement of a dynein-microtubule complex crosslinked with dna origami |
topic | Cell Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9232216/ https://www.ncbi.nlm.nih.gov/pubmed/35749159 http://dx.doi.org/10.7554/eLife.76357 |
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