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Unusual free oligosaccharides in human bovine and caprine milk

Free oligosaccharides are abundant macronutrients in milk and involved in prebiotic functions and antiadhesive binding of viruses and pathogenic bacteria to colonocytes. Despite the importance of these oligosaccharides, structural determination of oligosaccharides is challenging, and milk oligosacch...

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Autores principales: Weng, Wei-Chien, Liao, Hung-En, Huang, Shih-Pei, Tsai, Shang-Ting, Hsu, Hsu-Chen, Liew, Chia Yen, Gannedi, Veeranjaneyulu, Hung, Shang-Cheng, Ni, Chi-Kung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9232581/
https://www.ncbi.nlm.nih.gov/pubmed/35750794
http://dx.doi.org/10.1038/s41598-022-15140-7
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author Weng, Wei-Chien
Liao, Hung-En
Huang, Shih-Pei
Tsai, Shang-Ting
Hsu, Hsu-Chen
Liew, Chia Yen
Gannedi, Veeranjaneyulu
Hung, Shang-Cheng
Ni, Chi-Kung
author_facet Weng, Wei-Chien
Liao, Hung-En
Huang, Shih-Pei
Tsai, Shang-Ting
Hsu, Hsu-Chen
Liew, Chia Yen
Gannedi, Veeranjaneyulu
Hung, Shang-Cheng
Ni, Chi-Kung
author_sort Weng, Wei-Chien
collection PubMed
description Free oligosaccharides are abundant macronutrients in milk and involved in prebiotic functions and antiadhesive binding of viruses and pathogenic bacteria to colonocytes. Despite the importance of these oligosaccharides, structural determination of oligosaccharides is challenging, and milk oligosaccharide biosynthetic pathways remain unclear. Oligosaccharide structures are conventionally determined using a combination of chemical reactions, exoglycosidase digestion, nuclear magnetic resonance spectroscopy, and mass spectrometry. Most reported free oligosaccharides are highly abundant and have lactose at the reducing end, and current oligosaccharide biosynthetic pathways in human milk are proposed based on these oligosaccharides. In this study, a new mass spectrometry technique, which can identify linkages, anomericities, and stereoisomers, was applied to determine the structures of free oligosaccharides in human, bovine, and caprine milk. Oligosaccharides that do not follow the current biosynthetic pathways and are not synthesized by any discovered enzymes were found, indicating the existence of undiscovered biosynthetic pathways and enzymes.
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spelling pubmed-92325812022-06-26 Unusual free oligosaccharides in human bovine and caprine milk Weng, Wei-Chien Liao, Hung-En Huang, Shih-Pei Tsai, Shang-Ting Hsu, Hsu-Chen Liew, Chia Yen Gannedi, Veeranjaneyulu Hung, Shang-Cheng Ni, Chi-Kung Sci Rep Article Free oligosaccharides are abundant macronutrients in milk and involved in prebiotic functions and antiadhesive binding of viruses and pathogenic bacteria to colonocytes. Despite the importance of these oligosaccharides, structural determination of oligosaccharides is challenging, and milk oligosaccharide biosynthetic pathways remain unclear. Oligosaccharide structures are conventionally determined using a combination of chemical reactions, exoglycosidase digestion, nuclear magnetic resonance spectroscopy, and mass spectrometry. Most reported free oligosaccharides are highly abundant and have lactose at the reducing end, and current oligosaccharide biosynthetic pathways in human milk are proposed based on these oligosaccharides. In this study, a new mass spectrometry technique, which can identify linkages, anomericities, and stereoisomers, was applied to determine the structures of free oligosaccharides in human, bovine, and caprine milk. Oligosaccharides that do not follow the current biosynthetic pathways and are not synthesized by any discovered enzymes were found, indicating the existence of undiscovered biosynthetic pathways and enzymes. Nature Publishing Group UK 2022-06-24 /pmc/articles/PMC9232581/ /pubmed/35750794 http://dx.doi.org/10.1038/s41598-022-15140-7 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Weng, Wei-Chien
Liao, Hung-En
Huang, Shih-Pei
Tsai, Shang-Ting
Hsu, Hsu-Chen
Liew, Chia Yen
Gannedi, Veeranjaneyulu
Hung, Shang-Cheng
Ni, Chi-Kung
Unusual free oligosaccharides in human bovine and caprine milk
title Unusual free oligosaccharides in human bovine and caprine milk
title_full Unusual free oligosaccharides in human bovine and caprine milk
title_fullStr Unusual free oligosaccharides in human bovine and caprine milk
title_full_unstemmed Unusual free oligosaccharides in human bovine and caprine milk
title_short Unusual free oligosaccharides in human bovine and caprine milk
title_sort unusual free oligosaccharides in human bovine and caprine milk
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9232581/
https://www.ncbi.nlm.nih.gov/pubmed/35750794
http://dx.doi.org/10.1038/s41598-022-15140-7
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