Base editing sensor libraries for high-throughput engineering and functional analysis of cancer-associated single nucleotide variants

Base editing (BE) can be applied to characterize single nucleotide variants (SNVs) of unknown function, yet defining effective combinations of single guide RNAs (sgRNAs) and base editors remains challenging. Here, we describe modular BE-activity ‘sensors’ that link sgRNAs and cognate target sites in...

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Detalles Bibliográficos
Autores principales: Sánchez-Rivera, Francisco J., Diaz, Bianca J., Kastenhuber, Edward R., Schmidt, Henri, Katti, Alyna, Kennedy, Margaret, Tem, Vincent, Ho, Yu-Jui, Leibold, Josef, Paffenholz, Stella V., Barriga, Francisco M., Chu, Kevan, Goswami, Sukanya, Wuest, Alexandra N., Simon, Janelle M., Tsanov, Kaloyan M., Chakravarty, Debyani, Zhang, Hongxin, Leslie, Christina S., Lowe, Scott W., Dow, Lukas E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9232935/
https://www.ncbi.nlm.nih.gov/pubmed/35165384
http://dx.doi.org/10.1038/s41587-021-01172-3

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