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Gut transcriptome of two bark beetle species stimulated with the same kairomones reveals molecular differences in detoxification pathways

Dendroctonus bark beetles are the most destructive agents in coniferous forests. These beetles come into contact with the toxic compounds of their host's chemical defenses throughout their life cycle, some of which are also used by the insects as kairomones to select their host trees during the...

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Autores principales: Torres-Banda, Verónica, Obregón-Molina, Gabriel, Viridiana Soto-Robles, L., Albores-Medina, Arnulfo, Fernanda López, María, Zúñiga, Gerardo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Research Network of Computational and Structural Biotechnology 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9233182/
https://www.ncbi.nlm.nih.gov/pubmed/35782727
http://dx.doi.org/10.1016/j.csbj.2022.06.029
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author Torres-Banda, Verónica
Obregón-Molina, Gabriel
Viridiana Soto-Robles, L.
Albores-Medina, Arnulfo
Fernanda López, María
Zúñiga, Gerardo
author_facet Torres-Banda, Verónica
Obregón-Molina, Gabriel
Viridiana Soto-Robles, L.
Albores-Medina, Arnulfo
Fernanda López, María
Zúñiga, Gerardo
author_sort Torres-Banda, Verónica
collection PubMed
description Dendroctonus bark beetles are the most destructive agents in coniferous forests. These beetles come into contact with the toxic compounds of their host's chemical defenses throughout their life cycle, some of which are also used by the insects as kairomones to select their host trees during the colonization process. However, little is known about the molecular mechanisms by which the insects counteract the toxicity of these compounds. Here, two sibling species of bark beetles, D. valens and D. rhizophagus, were stimulated with vapors of a blend of their main kairomones (α-pinene, β-pinene and 3-carene), in order to compare the transcriptional response of their gut. A total of 48 180 unigenes were identified in D. valens and 43 704 in D. rhizophagus, in response to kairomones blend. The analysis of differential gene expression showed a transcriptional response in D. valens (739 unigenes, 0.58–10.36 Log2FC) related to digestive process and in D. rhizophagus (322 unigenes 0.87–13.08 Log2FC) related to xenobiotics metabolism. The expression profiles of detoxification genes mainly evidenced the up-regulation of COEs and GSTs in D. valens, and the up-regulation of P450s in D. rhizophagus. Results suggest that terpenes metabolism comes accompanied by an integral hormetic response, result of compensatory mechanisms, including the activation of other metabolic pathways, to ensure the supply of energy and the survival of organisms which is specific for each species, according to its life history and ecological strategy.
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spelling pubmed-92331822022-07-01 Gut transcriptome of two bark beetle species stimulated with the same kairomones reveals molecular differences in detoxification pathways Torres-Banda, Verónica Obregón-Molina, Gabriel Viridiana Soto-Robles, L. Albores-Medina, Arnulfo Fernanda López, María Zúñiga, Gerardo Comput Struct Biotechnol J Research Article Dendroctonus bark beetles are the most destructive agents in coniferous forests. These beetles come into contact with the toxic compounds of their host's chemical defenses throughout their life cycle, some of which are also used by the insects as kairomones to select their host trees during the colonization process. However, little is known about the molecular mechanisms by which the insects counteract the toxicity of these compounds. Here, two sibling species of bark beetles, D. valens and D. rhizophagus, were stimulated with vapors of a blend of their main kairomones (α-pinene, β-pinene and 3-carene), in order to compare the transcriptional response of their gut. A total of 48 180 unigenes were identified in D. valens and 43 704 in D. rhizophagus, in response to kairomones blend. The analysis of differential gene expression showed a transcriptional response in D. valens (739 unigenes, 0.58–10.36 Log2FC) related to digestive process and in D. rhizophagus (322 unigenes 0.87–13.08 Log2FC) related to xenobiotics metabolism. The expression profiles of detoxification genes mainly evidenced the up-regulation of COEs and GSTs in D. valens, and the up-regulation of P450s in D. rhizophagus. Results suggest that terpenes metabolism comes accompanied by an integral hormetic response, result of compensatory mechanisms, including the activation of other metabolic pathways, to ensure the supply of energy and the survival of organisms which is specific for each species, according to its life history and ecological strategy. Research Network of Computational and Structural Biotechnology 2022-06-16 /pmc/articles/PMC9233182/ /pubmed/35782727 http://dx.doi.org/10.1016/j.csbj.2022.06.029 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Torres-Banda, Verónica
Obregón-Molina, Gabriel
Viridiana Soto-Robles, L.
Albores-Medina, Arnulfo
Fernanda López, María
Zúñiga, Gerardo
Gut transcriptome of two bark beetle species stimulated with the same kairomones reveals molecular differences in detoxification pathways
title Gut transcriptome of two bark beetle species stimulated with the same kairomones reveals molecular differences in detoxification pathways
title_full Gut transcriptome of two bark beetle species stimulated with the same kairomones reveals molecular differences in detoxification pathways
title_fullStr Gut transcriptome of two bark beetle species stimulated with the same kairomones reveals molecular differences in detoxification pathways
title_full_unstemmed Gut transcriptome of two bark beetle species stimulated with the same kairomones reveals molecular differences in detoxification pathways
title_short Gut transcriptome of two bark beetle species stimulated with the same kairomones reveals molecular differences in detoxification pathways
title_sort gut transcriptome of two bark beetle species stimulated with the same kairomones reveals molecular differences in detoxification pathways
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9233182/
https://www.ncbi.nlm.nih.gov/pubmed/35782727
http://dx.doi.org/10.1016/j.csbj.2022.06.029
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