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The Effectiveness of Sperm Preparation Using Density Mini-Gradient and Single-Layer Centrifugation for Oligospermia Samples
BACKGROUND: Sperm preparation is an important step during assisted reproduction, and different assisted reproductive techniques have different sperm quality requirements. For intrauterine insemination (IUI), the total motile sperm count is a predictor of a patient’s fertility. OBJECTIVE: The aim of...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Academy of Medical sciences
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9233458/ https://www.ncbi.nlm.nih.gov/pubmed/35774841 http://dx.doi.org/10.5455/aim.2022.30.100-104 |
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author | Hoa, Nguyen Thanh Son, Nguyen Khang Hoa, Tran-Thi Phuong Giang, Mai-Thi Dung, Le Ngoc Duc, Nguyen Minh Ha, Nguyen Manh |
author_facet | Hoa, Nguyen Thanh Son, Nguyen Khang Hoa, Tran-Thi Phuong Giang, Mai-Thi Dung, Le Ngoc Duc, Nguyen Minh Ha, Nguyen Manh |
author_sort | Hoa, Nguyen Thanh |
collection | PubMed |
description | BACKGROUND: Sperm preparation is an important step during assisted reproduction, and different assisted reproductive techniques have different sperm quality requirements. For intrauterine insemination (IUI), the total motile sperm count is a predictor of a patient’s fertility. OBJECTIVE: The aim of this study was to compare the sperm recovery rate and DNA fragmentation index (DFI) outcomes following density mini-gradient and single-layer centrifugation in preparation for intrauterine insemination (IUI). METHODS: A total of 30 semen samples with concentrations under 15 million cells/ml were obtained, and each sample was divided into 3 aliquots, with each aliquot subjected to 1 of 3 separation methods: mini-gradient, single-layer using a 90% density layer (single 90-layer), and single-layer using a 45% density layer (single 45-layer). Total sperm motility and sperm recovery rates were compared before and after preparation using each method. RESULTS: The sperm concentration obtained using single 45-layer was higher than the other groups (p<0.05), but sperm motility was higher using the mini-gradient and single 90-layer methods higher than the single 45-layer method (p<0.05). The recovered sperm motility rates for the mini-gradient, single 90-layer, and single 45-layer methods were 57.6% ± 20.6%, 62.8% ± 18.5%, and 78.7% ± 12.4%, respectively, indicating a better outcome for the single 45-layer method than for the other methods. CONCLUSION: All of these methods can be applied to sperm preparation for IUI, and the optimal method can be selected based on initial sperm quality to collect sperm with good motility and DNA integrity to achieve a satisfactory pregnancy rate. |
format | Online Article Text |
id | pubmed-9233458 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Academy of Medical sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-92334582022-06-29 The Effectiveness of Sperm Preparation Using Density Mini-Gradient and Single-Layer Centrifugation for Oligospermia Samples Hoa, Nguyen Thanh Son, Nguyen Khang Hoa, Tran-Thi Phuong Giang, Mai-Thi Dung, Le Ngoc Duc, Nguyen Minh Ha, Nguyen Manh Acta Inform Med Original Paper BACKGROUND: Sperm preparation is an important step during assisted reproduction, and different assisted reproductive techniques have different sperm quality requirements. For intrauterine insemination (IUI), the total motile sperm count is a predictor of a patient’s fertility. OBJECTIVE: The aim of this study was to compare the sperm recovery rate and DNA fragmentation index (DFI) outcomes following density mini-gradient and single-layer centrifugation in preparation for intrauterine insemination (IUI). METHODS: A total of 30 semen samples with concentrations under 15 million cells/ml were obtained, and each sample was divided into 3 aliquots, with each aliquot subjected to 1 of 3 separation methods: mini-gradient, single-layer using a 90% density layer (single 90-layer), and single-layer using a 45% density layer (single 45-layer). Total sperm motility and sperm recovery rates were compared before and after preparation using each method. RESULTS: The sperm concentration obtained using single 45-layer was higher than the other groups (p<0.05), but sperm motility was higher using the mini-gradient and single 90-layer methods higher than the single 45-layer method (p<0.05). The recovered sperm motility rates for the mini-gradient, single 90-layer, and single 45-layer methods were 57.6% ± 20.6%, 62.8% ± 18.5%, and 78.7% ± 12.4%, respectively, indicating a better outcome for the single 45-layer method than for the other methods. CONCLUSION: All of these methods can be applied to sperm preparation for IUI, and the optimal method can be selected based on initial sperm quality to collect sperm with good motility and DNA integrity to achieve a satisfactory pregnancy rate. Academy of Medical sciences 2022-06 /pmc/articles/PMC9233458/ /pubmed/35774841 http://dx.doi.org/10.5455/aim.2022.30.100-104 Text en © 2022 Nguyen Thanh Hoa, Nguyen Khang Son, Tran-Thi Phuong Hoa, Mai-Thi Giang, Le Ngoc Dung, Nguyen Minh Duc, Nguyen Manh Ha https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) ) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Paper Hoa, Nguyen Thanh Son, Nguyen Khang Hoa, Tran-Thi Phuong Giang, Mai-Thi Dung, Le Ngoc Duc, Nguyen Minh Ha, Nguyen Manh The Effectiveness of Sperm Preparation Using Density Mini-Gradient and Single-Layer Centrifugation for Oligospermia Samples |
title | The Effectiveness of Sperm Preparation Using Density Mini-Gradient and Single-Layer Centrifugation for Oligospermia Samples |
title_full | The Effectiveness of Sperm Preparation Using Density Mini-Gradient and Single-Layer Centrifugation for Oligospermia Samples |
title_fullStr | The Effectiveness of Sperm Preparation Using Density Mini-Gradient and Single-Layer Centrifugation for Oligospermia Samples |
title_full_unstemmed | The Effectiveness of Sperm Preparation Using Density Mini-Gradient and Single-Layer Centrifugation for Oligospermia Samples |
title_short | The Effectiveness of Sperm Preparation Using Density Mini-Gradient and Single-Layer Centrifugation for Oligospermia Samples |
title_sort | effectiveness of sperm preparation using density mini-gradient and single-layer centrifugation for oligospermia samples |
topic | Original Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9233458/ https://www.ncbi.nlm.nih.gov/pubmed/35774841 http://dx.doi.org/10.5455/aim.2022.30.100-104 |
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