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Determination of total phenolic content and antioxidant activity of Commiphora mollis (Oliv.) Engl. resin

In this study, total phenolic contents (TPC) and antioxidant activity of Commiphora mollis (Oliv.) Engl. (Burseraceae) resin were investigated. The resin was extracted using petroleum ether, chloroform, and methanol to give 27.46 ± 0.48, 46.56 ± 0.42, and 53.00 ± 1.39% extractable solids, respective...

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Autores principales: Molole, Guyo Jilo, Gure, Abera, Abdissa, Negera
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9233799/
https://www.ncbi.nlm.nih.gov/pubmed/35752844
http://dx.doi.org/10.1186/s13065-022-00841-x
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author Molole, Guyo Jilo
Gure, Abera
Abdissa, Negera
author_facet Molole, Guyo Jilo
Gure, Abera
Abdissa, Negera
author_sort Molole, Guyo Jilo
collection PubMed
description In this study, total phenolic contents (TPC) and antioxidant activity of Commiphora mollis (Oliv.) Engl. (Burseraceae) resin were investigated. The resin was extracted using petroleum ether, chloroform, and methanol to give 27.46 ± 0.48, 46.56 ± 0.42, and 53.00 ± 1.39% extractable solids, respectively. The Folin–Ciocalteu (F–C) redox assay was optimized considering relevant parameters such as reaction time, maximum wavelength, and sample dilution effect before the determination of TPC. The concentration of antioxidants necessary to decrease by 50% the initial concentration of DPPH (EC(50)) was determined at 60 min. The reaction kinetics was analyzed using the pseudo-first-order kinetics model. For the F–C assay, the optimum conditions for the maximum absorbance and analysis time were 760 nm and 30 min, respectively. Under these conditions, the method exhibited good sensitivity and linear instrumental responses over wide ranges of concentrations. The highest TPC;168.27 ± 3.44, 137.43 ± 1.32, and 136.16 ± 0.42 mgGAE/g were recorded in the diluted samples (500 µg/mL) of methanol, chloroform, and petroleum ether extracts, respectively. By using different concentrations of the test sample, exhaustive reduction of phenolics and/or antioxidant substrates was achieved. Regarding the DPPH radical scavenging capacity, the EC(50) values for methanol, chloroform, and petroleum ether extracts were 295.03 ± 3.55, 342.75 ± 9.72, and 353.69 ± 7.30 µg/mL, respectively. The standard (l-ascorbic acid), however, exhibited much lower EC(50) value (44.72 ± 0.48 µg/mL). The methanol extracts showed kinetic behavior (k(2) values,115.08 to 53.28 M(−1) s(−1); steady-state time, < 29 min) closer to that of l-ascorbic acid (k(2) values, 190 to 109 M(−1) s(−1); steady-state time, < 16 min), than other two extracts (k(2) values,14 to 28 M(−1) s(−1); steady-state time, 63 to 130 min). For all tested samples, the rate of the DPPH radical scavenging increases with concentration from 50 to 250 µg/mL. The current study demonstrated that the polar solvent (methanol) extract has a better F–C reducing capacity and DPPH radical scavenging activity than the nonpolar solvents extracts. This could be due to phenolics and other oxidation substrates extracted by methanol from the C. mollis resin. For a better understanding of the antioxidant constituents of the resin, a further study including isolation of its compounds is recommended.
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spelling pubmed-92337992022-06-27 Determination of total phenolic content and antioxidant activity of Commiphora mollis (Oliv.) Engl. resin Molole, Guyo Jilo Gure, Abera Abdissa, Negera BMC Chem Research In this study, total phenolic contents (TPC) and antioxidant activity of Commiphora mollis (Oliv.) Engl. (Burseraceae) resin were investigated. The resin was extracted using petroleum ether, chloroform, and methanol to give 27.46 ± 0.48, 46.56 ± 0.42, and 53.00 ± 1.39% extractable solids, respectively. The Folin–Ciocalteu (F–C) redox assay was optimized considering relevant parameters such as reaction time, maximum wavelength, and sample dilution effect before the determination of TPC. The concentration of antioxidants necessary to decrease by 50% the initial concentration of DPPH (EC(50)) was determined at 60 min. The reaction kinetics was analyzed using the pseudo-first-order kinetics model. For the F–C assay, the optimum conditions for the maximum absorbance and analysis time were 760 nm and 30 min, respectively. Under these conditions, the method exhibited good sensitivity and linear instrumental responses over wide ranges of concentrations. The highest TPC;168.27 ± 3.44, 137.43 ± 1.32, and 136.16 ± 0.42 mgGAE/g were recorded in the diluted samples (500 µg/mL) of methanol, chloroform, and petroleum ether extracts, respectively. By using different concentrations of the test sample, exhaustive reduction of phenolics and/or antioxidant substrates was achieved. Regarding the DPPH radical scavenging capacity, the EC(50) values for methanol, chloroform, and petroleum ether extracts were 295.03 ± 3.55, 342.75 ± 9.72, and 353.69 ± 7.30 µg/mL, respectively. The standard (l-ascorbic acid), however, exhibited much lower EC(50) value (44.72 ± 0.48 µg/mL). The methanol extracts showed kinetic behavior (k(2) values,115.08 to 53.28 M(−1) s(−1); steady-state time, < 29 min) closer to that of l-ascorbic acid (k(2) values, 190 to 109 M(−1) s(−1); steady-state time, < 16 min), than other two extracts (k(2) values,14 to 28 M(−1) s(−1); steady-state time, 63 to 130 min). For all tested samples, the rate of the DPPH radical scavenging increases with concentration from 50 to 250 µg/mL. The current study demonstrated that the polar solvent (methanol) extract has a better F–C reducing capacity and DPPH radical scavenging activity than the nonpolar solvents extracts. This could be due to phenolics and other oxidation substrates extracted by methanol from the C. mollis resin. For a better understanding of the antioxidant constituents of the resin, a further study including isolation of its compounds is recommended. Springer International Publishing 2022-06-25 /pmc/articles/PMC9233799/ /pubmed/35752844 http://dx.doi.org/10.1186/s13065-022-00841-x Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Molole, Guyo Jilo
Gure, Abera
Abdissa, Negera
Determination of total phenolic content and antioxidant activity of Commiphora mollis (Oliv.) Engl. resin
title Determination of total phenolic content and antioxidant activity of Commiphora mollis (Oliv.) Engl. resin
title_full Determination of total phenolic content and antioxidant activity of Commiphora mollis (Oliv.) Engl. resin
title_fullStr Determination of total phenolic content and antioxidant activity of Commiphora mollis (Oliv.) Engl. resin
title_full_unstemmed Determination of total phenolic content and antioxidant activity of Commiphora mollis (Oliv.) Engl. resin
title_short Determination of total phenolic content and antioxidant activity of Commiphora mollis (Oliv.) Engl. resin
title_sort determination of total phenolic content and antioxidant activity of commiphora mollis (oliv.) engl. resin
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9233799/
https://www.ncbi.nlm.nih.gov/pubmed/35752844
http://dx.doi.org/10.1186/s13065-022-00841-x
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