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Identification of Cigarette Smoking-Related Novel Biomarkers in Lung Adenocarcinoma
OBJECTIVE: The aims of this study were to screen the gene mutations that are able to predict the risk of cigarette smoking-related lung adenocarcinoma (LUAD) and to evaluate its prognostic significance. METHODS: Clinical data and genetic information were retrieved from the TCGA database, and the pat...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9234045/ https://www.ncbi.nlm.nih.gov/pubmed/35769670 http://dx.doi.org/10.1155/2022/9170722 |
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author | Zhang, Yuan Wang, Qiong Zhu, Ting Chen, Hui |
author_facet | Zhang, Yuan Wang, Qiong Zhu, Ting Chen, Hui |
author_sort | Zhang, Yuan |
collection | PubMed |
description | OBJECTIVE: The aims of this study were to screen the gene mutations that are able to predict the risk of cigarette smoking-related lung adenocarcinoma (LUAD) and to evaluate its prognostic significance. METHODS: Clinical data and genetic information were retrieved from the TCGA database, and the patients with LUAD were divided into three groups including never smoking, light smoking, and heavy smoking according to cigarette smoking dose. Differentially mutated genes (DMGs) of each group were analyzed. At the same time, the function of DMGs in three smoking groups was evaluated by GO function and KEGG pathway analysis. The driver genes and protein variation effect of DMGs were performed to further screen key genes. The survival characteristics of the gene expression and mutation of those genes were analyzed and plotted to visualize by the Kaplan-Meier model. RESULT: The DMGs for different smoking doses were identified. The driver and deleterious mutation in the DMGs were screened and gene interaction network was constructed. The DMGs with driver mutations and deleterious mutations that were associated with the overall survival in the heavy smoking patients were considered as the candidate genes for novel markers of smoking-related LUAD. The final novel risk factor gene was identified as MYH7 and the high express of MYH7 in LUAD correlation with patients' gender, lymph node metastasis, T stage, and clinical stage. CONCLUSIONS: In summary, it can be concluded that MYH7 is a novel biomarker for heavy smoking-related LUAD and it is significantly correlated with the prognosis of lung cancer and is related to the clinical characteristics of lung cancer. |
format | Online Article Text |
id | pubmed-9234045 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-92340452022-06-28 Identification of Cigarette Smoking-Related Novel Biomarkers in Lung Adenocarcinoma Zhang, Yuan Wang, Qiong Zhu, Ting Chen, Hui Biomed Res Int Research Article OBJECTIVE: The aims of this study were to screen the gene mutations that are able to predict the risk of cigarette smoking-related lung adenocarcinoma (LUAD) and to evaluate its prognostic significance. METHODS: Clinical data and genetic information were retrieved from the TCGA database, and the patients with LUAD were divided into three groups including never smoking, light smoking, and heavy smoking according to cigarette smoking dose. Differentially mutated genes (DMGs) of each group were analyzed. At the same time, the function of DMGs in three smoking groups was evaluated by GO function and KEGG pathway analysis. The driver genes and protein variation effect of DMGs were performed to further screen key genes. The survival characteristics of the gene expression and mutation of those genes were analyzed and plotted to visualize by the Kaplan-Meier model. RESULT: The DMGs for different smoking doses were identified. The driver and deleterious mutation in the DMGs were screened and gene interaction network was constructed. The DMGs with driver mutations and deleterious mutations that were associated with the overall survival in the heavy smoking patients were considered as the candidate genes for novel markers of smoking-related LUAD. The final novel risk factor gene was identified as MYH7 and the high express of MYH7 in LUAD correlation with patients' gender, lymph node metastasis, T stage, and clinical stage. CONCLUSIONS: In summary, it can be concluded that MYH7 is a novel biomarker for heavy smoking-related LUAD and it is significantly correlated with the prognosis of lung cancer and is related to the clinical characteristics of lung cancer. Hindawi 2022-06-19 /pmc/articles/PMC9234045/ /pubmed/35769670 http://dx.doi.org/10.1155/2022/9170722 Text en Copyright © 2022 Yuan Zhang et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Zhang, Yuan Wang, Qiong Zhu, Ting Chen, Hui Identification of Cigarette Smoking-Related Novel Biomarkers in Lung Adenocarcinoma |
title | Identification of Cigarette Smoking-Related Novel Biomarkers in Lung Adenocarcinoma |
title_full | Identification of Cigarette Smoking-Related Novel Biomarkers in Lung Adenocarcinoma |
title_fullStr | Identification of Cigarette Smoking-Related Novel Biomarkers in Lung Adenocarcinoma |
title_full_unstemmed | Identification of Cigarette Smoking-Related Novel Biomarkers in Lung Adenocarcinoma |
title_short | Identification of Cigarette Smoking-Related Novel Biomarkers in Lung Adenocarcinoma |
title_sort | identification of cigarette smoking-related novel biomarkers in lung adenocarcinoma |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9234045/ https://www.ncbi.nlm.nih.gov/pubmed/35769670 http://dx.doi.org/10.1155/2022/9170722 |
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